Why Cielo?

Azure Cielo qPCR system

For faster qPCR that's unparalleled in:

➡ Speed
➡ Flexibility
➡ Sensitivity
➡ Reproducibility
➡ Simplicity

EXPERIENCE FASTER qPCR

Fill out this form to receive special 2024 pricing today

HOW CIELO IS DOING BETTER qPCR

Simplicity

With Cielo, you can start your run at the bench and view results from anywhere.

Easily connect to your Cielo via Wi-Fi, ethernet, USB or LAN. Connectivity to Wi-Fi, USB, e-mail, and external PCs allow you to easily export your data. Get notified after each completed run and even remotely access protocols and files.

Flexibility

Up to 6 fluorescent channels allow for flexible dye usage and broad application flexibility.

Novel fiber optic detection system allows for enhanced sensitivity and speed. The option of 3 or 6 standard channels allows you to analyze a variety of commercially available dyes.

Sensitivity

Individual illumination and scanning of each well allows for specific and precise detection of qPCR signals

Unlike systems that use whole plate imaging, Cielo uses in-well excitation and detection so no light falls outside of the well, reducing background noise.

Because the excitation light path length is the same well-to-well, Cielo does not require reference dyes, freeing one additional channel for another target.

Reproducibility​

Optics and thermal block designed to deliver the same uniformity and reproducibility over and over again

Results from the Cielo are easily repeatable across runs. 1.3-fold differences in gene expression can be detected.

COMPARE qPCR SYSTEMS

FeaturesCielo 6 qPCR SystemCielo 3 qPCR System
Sample capacity (Wells)96 wells96 wells
Reaction Volume1-50 uL (1-65 recommended)1-50 uL (1-65 recommended)
Dynamic Range10 Logs10 Logs
Excitation SourceLED Excitation range: 450-730 nmLED Excitation range: 450-730 nm, 610-700 nm
Detection Channels63
MultiplexingUp to 3 targetsUp to 6 targets
Custom Dye/Chemistry
Onboard Memory32 GB Equivalent to 20,000 experiments32 GB Equivalent to 20,000 experiments
TouchscreenColorColor
ConnectivityUSB, Wifi, EthernetUSB, Wifi, Ethernet
IVDYesYes

Other dyes/probes are possible depending on the system configuration

MORE Reasons to Choose Cielo

Fast Results

  • Unique fiber optics based qPCR detection allowing a complete 96-well scan in 1.5 seconds per channel
  • Maximum sample ramp rate of 6°C/sec

Results Emailed to You

  • Get notified immediately after each completion of each run through email
  • Easily export data via Wi-Fi, ethernet, USB or LAN

Open Platform

  • Open platform compatible with a vast variety of dye/probe based chemistries
  • Third-party dye compatible

High Sensitivity

  • Detection sensitivity of up to 1 copy of amplified DNA

qPCR Your Way

  • Available in either 3 or 6 optical channel models to give you options for multiplex analysis

  • Ability to set up a temperature gradient with 12 zones

Durable

  • Tested for >1000 continuous experiments

Cielo Customers Include

Compatible with Commercially available Dyes

ParameterCielo 6Cielo 3
Sample capacity (Wells)96 wells
Compatible Plates/Tubes96-well plates and tubes, 8- or 12-well strips and single tubes
Reaction Volume1-50 uL (1-65 recommended)
Dynamic Range10 Logs
Excitation SourceLED Excitation range: 450-730 nmLED Excitation range: 450-730 nm, 610-700 nm
Detection Channels63
MultiplexingUp to 3 targetsUp to 6 targets
Reaction Temperature4°C to 99.9°C
Max Block Ramp Rate6°C/sec
Average Sample Ramp Rate4°C/Sec
Temperature Uniformity±0.2°C
Temperature Accuracy±0.1°C
Custom Dye/Chemistry
Onboard Memory32 GB Equivalent to 20,000 experiments
Unit dimensions (W x H x D)​30.5 cm x 50.8 cm x 43.2 cm / 12” x 20” x 17"
Weight​22.7kg / 50 lbs
Power requirements100-240 VAC, 8A; Consumption: 0.8kW (800 Watts)
ConnectivityUSB, Wifi, Ethernet
CertificationsCE Certified, TUV Certified

Other dyes/probes are possible depending on the system configuration

Supported Applications

  • Quantitative and qualitative gene expression analysis​
  • miRNA analysis​
  • Genetic mapping​
  • Genetic fingerprinting​
  • NGS library quantification​
  • 3 or 6 channel multiplex ability​
  • Pathogen quantification​
  • And more!

Literature

Document TypeDescription
BrochureView the Cielo BrochureDOWNLOAD
Application NoteOptical Design Eliminates the Need for ROX Passive Reference DyeDOWNLOAD
Application NoteSensitive and Accurate Single-Copy Amplicon DetectionDOWNLOAD
Application NoteData for 6-Channel Multiplex qPCRDOWNLOAD
Application NoteAzure Cielo 6 Compatibility with GenDx KMRtype and KMRtrack WorkflowsDOWNLOAD
Application NoteDetection of SARS-CoV-2 in Municipal WastewaterDOWNLOAD
Application NoteAdding Dyes and Color CompensationDOWNLOAD

SPECIAL PRICING AVAILABLE

Take advantage of a special qPCR promotional package (includes Cielo 6 and Cielo Manager Software, with an optional, additional laptop), now until the end of the year.

Shopping cart0
There are no products in the cart!
Continue shopping