Azure BiosystemsHigh-quality and affordable imaging systems, qPCR systems, and reagents.
Azure Biosystems
Easily connect to your Cielo via Wi-Fi, ethernet, USB or LAN. Connectivity to Wi-Fi, USB, e-mail, and external PCs allow you to easily export your data. Get notified after each completed run and even remotely access protocols and files.
Novel fiber optic detection system allows for enhanced sensitivity and speed. The option of 3 or 6 standard channels allows you to analyze a variety of commercially available dyes.
Rox is not required, so you can use it as a probe.
Unlike systems that use whole plate imaging, no excitation light falls outside of the well, reducing excitation of non-well regions. This reduces potential background.
Results from the Cielo are easily repeatable across runs. Two-fold differences in gene expression can be detected.
CHANNEL 1
EX: 475 ± 14
EM: 524 ± 12
DYES: SYBR™ Green, EvaGreen™, FAM™
CHANNEL 2
EX: 527 ± 10
EM: 565 ± 12
DYES: VIC®, HEX™, JOE™, CAL Fluor® 540, CAL Fluor®, Orange 560
CHANNEL 3
NOT AVAILABLE
CHANNEL 4
NOT AVAILABLE
CHANNEL 5
EX: 623 ± 12
EM: 676 ± 18.5
DYES: CY®5, Quasar®670, Liz®, Mustang Purple®
CHANNEL 6
NOT AVAILABLE
CHANNEL 1
EX: 475 ± 14
EM: 524 ± 12
DYES: SYBR™ Green, EvaGreen™, FAM™
CHANNEL 2
EX: 527 ± 10
EM: 565 ± 12
DYES: VIC®, HEX™, JOE™, CAL Fluor® 540, CAL Fluor®, Orange 560
CHANNEL 3
EX: 572 ± 7.5
EM: 623 ± 12
DYES: TAMRA™, Cy3
CHANNEL 4
EX: 537 ± 13
EM: 583 ± 11
DYES: ROX™, TEX®615, CAL Fluor® Red 610
CHANNEL 5
EX: 623 ± 12
EM: 676 ± 18.5
DYES: CY®5, Quasar®670, Liz®, Mustang Purple®
CHANNEL 6
EX: 655 ± 7.5
EM: 711 ± 12.5
DYES: CY® 5.5, Quasar 705
Other dyes/probes are possible depending on the system configuration
| Document Type | Description | |
|---|---|---|
| Brochure | View the Cielo Brochure | DOWNLOAD |
| Application Note | Optical Design Eliminates the Need for ROX Passive Reference Dye | DOWNLOAD |
| Application Note | Sensitive and Accurate Single-Copy Amplicon Detection | DOWNLOAD |
| Application Note | Data for 6-Channel Multiplex qPCR | DOWNLOAD |
| Application Note | Azure Cielo 6 Compatibility with GenDx KMRtype and KMRtrack Workflows | DOWNLOAD |
