Need help?

 |

SIGN IN

Call (925) 307-7127
At this time our store is only available to customers within the United States.  Find distributors in your country.

Why Cielo?

For qPCR unparalleled in:
Flexibility Sensitivity Reproducibility Speed Simplicity

Request a Quote
Azure-Cielo_curves_lores-600x825

The Azure Biosystems™ Cielo™ 3 and 6 real-time PCR Systems are designed to offer smart protocols, high quality data, robust durability and an intuitive user experience.​

Our novel fiber optic detection system allows for enhanced sensitivity and speed. 3 or 6 standard channels let you analyze a variety of commercially available dyes, already in your workflow. Onboard software operated by a 10.2″ touchscreen minimizes footprint and saves you bench space.​

Easily connect to your Cielo via Wi-Fi, ethernet, USB or LAN. Get notified after each completed run and even remotely access protocols and files.

Key Benefits of the Azure Cielo Real-Time PCR

Flexibility

Up to 6 fluorescent channels allow for flexible dye usage and broad application flexibility. Additionally, Rox is not required, so you can use it as a probe.​

 
Sensitivity

Individual illumination and scanning of each well allows for specific and precise detection of qPCR signals. Unlike systems that use whole plate imaging, no excitation light falls outside of the well, reducing excitation of non-well regions. This reduces potential background​.

Reproducibility

The Cielo’s optics and thermal block are designed to deliver the same uniformity and reproducibility over at least 1000 qPCR experiments, giving you confidence in the reliability of the instrument.​​

Simplicity

Packaged into a compact system with a built in touch screen and intuitive software. Connectivity to Wi-Fi, USB, e-mail, and external PCs allow you to easily export your data.

Highlighted Product Features

  • Unique fiber optics based qPCR detection allowing a complete 96-well scan in 9 seconds​
  • Average Sample ramp rate of 4C/sec​
  • Available in either 3 or 6 optical channel options give you options for multiplex analysis​
  • Ability to setup 12 independent temperature gradient zones​
  • Detection sensitivity of up to 1 copy of amplified DNA​
  • Open platform compatible with a vast variety of dye/probe based chemistries​
  • Durable qPCR system tested for up to 1000 continuous experiments​
  • Easily export data via Wi-Fi, ethernet, USB or LAN. Get notified after each run completion​
REQUEST A QUOTE

Azure Cielo Dye Compatibility

Azure Cielo 3

CHANNEL 1

EX: 475 ± 14
EM: 524 ± 12
DYES: SYBR™ Green, EvaGreen™, FAM™

CHANNEL 2

EX: 527 ± 10
EM: 565 ± 12
DYES: VIC®, HEX™, JOE™, CAL Fluor® 540, CAL Fluor®, Orange 560

CHANNEL 3

NOT AVAILABLE

CHANNEL 4

NOT AVAILABLE

CHANNEL 5

EX: 623 ± 12
EM: 676 ± 18.5
DYES: CY®5, Quasar®670, Liz®, Mustang Purple®

CHANNEL 6

NOT AVAILABLE

Azure Cielo 6

CHANNEL 1

EX: 475 ± 14
EM: 524 ± 12
DYES: SYBR™ Green, EvaGreen™, FAM™

CHANNEL 2

EX: 527 ± 10
EM: 565 ± 12
DYES: VIC®, HEX™, JOE™, CAL Fluor® 540, CAL Fluor®, Orange 560

CHANNEL 3

EX: 572 ± 7.5
EM: 623 ± 12
DYES: TAMRA™, Cy3

CHANNEL 4

EX: 537 ± 13
EM: 583 ± 11
DYES: ROX™, TEX®615, CAL Fluor® Red 610

CHANNEL 5

EX: 623 ± 12
EM: 676 ± 18.5
DYES: CY®5, Quasar®670, Liz®, Mustang Purple®

CHANNEL 6

EX: 655 ± 7.5
EM: 711 ± 12.5
DYES: CY® 5.5, Quasar 705

Other dyes/probes are possible depending on the system configuration

REQUEST A QUOTE

Product Applications

  • Quantitative and qualitative gene expression analysis​
  • miRNA analysis​
  • Genetic mapping​
  • Genetic fingerprinting​
  • NGS library quantification​
  • 3 or 6 channel multiplex ability​
  • Pathogen quantification​
  • And more!

Literature

Document TypeDescription
BrochureView the Cielo BrochureDOWNLOAD
Application NoteOptical Design Eliminates the Need for ROX Passive Reference DyeDOWNLOAD
Application NoteSensitive and Accurate Single-Copy Amplicon DetectionDOWNLOAD
Application NoteData for 6-Channel Multiplex qPCRDOWNLOAD

Azure Cielo 6 Real Time qPCR System

Peter Cheung

Application Area: Radioisotpe RNA gel scanning

"The scanner is definitly the top model available on the market. It starts up fast, and doesnt need to warm up. The scanning is very sharp and time saving. It also gives a wide range of intensity dynamics. This machine gives us excellent publishing grade results. We recently relocated the machine, and our sales and service care manager Nicole gave us a lot of instruction on transporting and hand on hand calibration. She is very kind and responsible. We are all looking forward to cooperating with her in our coming new lab next year."

John Streicher

Application Area:Imaging western blots, in-cell westerns, tissue samples

"This is a high quality imager that has a strong value for the usability vs. price. The imager is highly programmable, supporting multiple applications, and returns sensitive and high quality data. The imager has also been reliable, with no errors, breakdowns, or problems. Adding to this, the sales staff, particularly David Grothen, are fantastic. They have worked with us, got us a great deal, and have been very supportive post-purchase. Buy it, you won't be sorry!"

Ajakaiye Tomilade

Application Area:Analyze amino acids and other metabolites

"This product is highly effective for analyzing metabolites and it yields a good result in due time. It's easy to use and great value for your money. "

Alana Vans

Application Area:Laboratory imaging laser scanning system.

"A last generation instrument of the highest quality. Thanks to its laser scanning technology, it allows us to clearly capture images in our laboratory, thus achieving a high efficiency index for all our biomolecular investigations. The range of images is can produce is broad, which allows us to generate images and results from even the smallest samples. In addition, it has a friendly, updated and intuitive interface that allows a simple experience for the operator. I can conclude that the Sapphire Biomolecular Imager is a piece of equipment that any laboratory should have, I have not really found any negative feature so I recommend it."

Sharyn Rossi

Application Area:Neuroscience

"We purchased this imager to replace our obsolete typhoon scanner and could not be happier with the performance and ease of use. Equipped with multiple lasers, it is possible to probe for many proteins at high resolution and with great sensitivity. We primarily use the system for western blotting and fluorescent slide scanning and have had clear, publishable results. The software is easy to use and has many practical features. Customer service is amazing – they are always available, informative, and as scientists, they assist with troubleshooting experimental design and optimizing results. This product is highly recommended for anyone who routinely uses electrophoresis, cell-based, or slide-based analyses with fluorescent or chemiluminescent detection."

Minhua Dou

Application Area:Biological product quality control related products and services

"Sapphire RGBIR is a powerful and quality laser scanner in our laboratory. Its high-performance laser light sources, high-sensitivity detector and 10μm highest resolution gives us high-quality data; Its multiplex fluorescence channels and CCD-based function makes us can develop several methods to verify and get an accurate result. For us, the most important thing of the large precision instrument besides good quality, performance is service. Azure's good service is better than most of other manufacturers because of the professional regular service about twice a year and the technical support without delay when asked."

Read Full Reviews
Write a Review

Azure Biosystems
6747 Sierra Court, Suite A-B
Dublin, CA 94568
United States

Tel: (925) 307 7127
Fax: (925) 905-1816
Info@azurebiosystems.com
Facebook Twitter Linkedin Instagram

Newsletter

Products

Company

Support

Shopping cart
There are no products in the cart!
Continue shopping