Azure BiosystemsHigh-quality and affordable imaging systems, qPCR systems, and reagents.
Azure Biosystems
Easily connect to your Cielo via Wi-Fi, ethernet, USB or LAN. Connectivity to Wi-Fi, USB, e-mail, and external PCs allow you to easily export your data. Get notified after each completed run and even remotely access protocols and files.
Novel fiber optic detection system allows for enhanced sensitivity and speed. The option of 3 or 6 standard channels allows you to analyze a variety of commercially available dyes.
Unlike systems that use whole plate imaging, Cielo uses in-well excitation and detection so no light falls outside of the well, reducing background noise.
Because the excitation light path length is the same well-to-well, Cielo does not require reference dyes, freeing one additional channel for another target.
Results from the Cielo are easily repeatable across runs. 1.3-fold differences in gene expression can be detected.
Available in either 3 or 6 optical channel models to give you options for multiplex analysis
Ability to set up a temperature gradient with 12 zones
CHANNEL 1
EX: 475 ± 14
EM: 524 ± 12
DYES: SYBR™ Green, EvaGreen™, FAM™
CHANNEL 2
EX: 527 ± 10
EM: 565 ± 12
DYES: VIC®, HEX™, JOE™, CAL Fluor® 540, CAL Fluor®, Orange 560
CHANNEL 3
NOT AVAILABLE
CHANNEL 4
NOT AVAILABLE
CHANNEL 5
EX: 623 ± 12
EM: 676 ± 18.5
DYES: CY®5, Quasar®670, Liz®, Mustang Purple®
CHANNEL 6
NOT AVAILABLE
CHANNEL 1
EX: 475 ± 14
EM: 524 ± 12
DYES: SYBR™ Green, EvaGreen™, FAM™
CHANNEL 2
EX: 527 ± 10
EM: 565 ± 12
DYES: VIC®, HEX™, JOE™, CAL Fluor® 540, CAL Fluor®, Orange 560
CHANNEL 3
EX: 572 ± 7.5
EM: 623 ± 12
DYES: TAMRA™, Cy3
CHANNEL 4
EX: 537 ± 13
EM: 583 ± 11
DYES: ROX™, TEX®615, CAL Fluor® Red 610
CHANNEL 5
EX: 623 ± 12
EM: 676 ± 18.5
DYES: CY®5, Quasar®670, Liz®, Mustang Purple®
CHANNEL 6
EX: 655 ± 7.5
EM: 711 ± 12.5
DYES: CY® 5.5, Quasar 705
Other dyes/probes are possible depending on the system configuration
| Document Type | Description | |
|---|---|---|
| Brochure | View the Cielo Brochure | DOWNLOAD |
| Application Note | Optical Design Eliminates the Need for ROX Passive Reference Dye | DOWNLOAD |
| Application Note | Sensitive and Accurate Single-Copy Amplicon Detection | DOWNLOAD |
| Application Note | Data for 6-Channel Multiplex qPCR | DOWNLOAD |
| Application Note | Azure Cielo 6 Compatibility with GenDx KMRtype and KMRtrack Workflows | DOWNLOAD |
| Application Note | Detection of SARS-CoV-2 in Municipal Wastewater | DOWNLOAD |
| Application Note | Adding Dyes and Color Compensation | DOWNLOAD |
