Azure BiosystemsHigh-quality and affordable imaging systems, qPCR systems, and reagents.
Azure Biosystems
THE REACTION
– Image blots up to 420 DPI
THE IMAGER
– Features auto-focus, auto-light control, auto-image capture, and dynamic flat fielding
– Integrated touchscreen for ease of use and easy configuration with an external PC
– Ideal for fluorescent, chemiluminescent, ultraviolet, and visible imaging with a 4.8 OD range
– Equipped with high-resolution CCD cameras with fast lens options, adjustable optical and lens settings, adjustable height tray with auto-detection, and pre-calibrated focus
THE DATA
“Total protein staining (TPS) represents the actual loading amount more accurately than HKPs due to minor technical and biological variation. Further, the broad dynamic range of TPS solves the issue of HKPs that commonly fail to show loading differences above small loading amounts of 0.5-10 μg.”
Moritz CP. Tubulin or Not Tubulin: Heading Toward Total Protein Staining as Loading Control in Western Blots. Proteomics. 2017 Oct;17(20). doi: 10.1002/pmic.201600189. PMID: 28941183
| ECL WB | ECL WB | FLUORESCENCE WB | |
|---|---|---|---|
| Detection Method | Film | CCD Imager | Fluorescence Imager |
| # of Target Proteins | 2 Targets | 2 Targets | 2 Targets |
| Protein Markers (0.5-1uL for IR; 10uL for Chemi) | $2.84 | $2.84 | $0.28 |
| Secondary Antibody (1:10,000 for FL ; 1:20,000 for ECL) | $0.20 | $0.20 | $0.68 |
| ECL Substrate (0.1 ml/cm2) | $32.80 | $32.80 | $0.00 |
| Photographic Film (2-4 sheets/ membrane) | $9.96 | $0.00 | $0.00 |
| Stripping Buffer (20 ml/reaction) | $5.06 | $5.06 | $0.00 |
| Total Experiment Cost | $50.86 | $40.90 | $0.96 |
| Labor hours | 8.3 Hours per WB | 7.4 Hours per WB | 4.3 hours per WB |
Western Blot expenses based on the use of consumables. Calculations assume a 10x10cm Western Blot. Online prices, February 2021.
Unique near-infrared (NIR) lasers help keep signal high and background low. Our high-performance multiplex NIR lasers and filters deliver robust excitation energy, which maximizes emission strength for maximum sensitivity.
Harness the power to do multiple applications with one imager!
The Azure Imaging Systems’ laser technology offers two NIR detection channels along with three visible fluorescence (RGB) fluorescence channels. This allows you to study multiple proteins in a single assay, even if those targets overlap in molecular weight. You can also easily resolve and quantify co-migrating bands, such as phosphorylated versus pan-protein forms.
| AZURE SAPPHIRE ™ | LI-COR ODYSSEY® CLX® | AMERSHAM® TYPHOON® | ||
|---|---|---|---|---|
| Lens-Sample Distance | Shortest | Long (reflecting mirrors) |  | |
| Visible Fluorescence | | | ||
| Chemiluminescence | | |||
| Phosphor Imaging | | |||
| Densitometry | | | ||
| Resolution | 10µm | 21µm | 10µm | |
| Price | $ | $ | $$ |
Quantitative Western blots require the normalization of signal to an internal loading control. Guidelines from leading journals state that total protein normalization is preferred over normalization to housekeeping proteins.
| Document Type | Description | |
|---|---|---|
| Brochure | Sapphire Biomolecular Imager | DOWNLOAD |
| Brochure | How Do You View-Infographic | DOWNLOAD |
| Brochure | Sapphire Applications Overview | DOWNLOAD |
| Application Note | Three-Color Western Blots with AzureSpectra Antibodies | DOWNLOAD |
| Application Note | Imaging Three-color Western Blots with the Azure c600 | DOWNLOAD |
| Application Note | Accurate Western Blot Normalization with AzureRed Fluorescent Protein Stain | DOWNLOAD |
| Application Note | Phosphorylated Protein Detection is More Efficient by Fluorescent Western Blot | DOWNLOAD |
| Application Note | Increasing Assay Efficiency with Four Color Detection | DOWNLOAD |
Azure Sapphire Video
pERK Detection via ICW Video
