Western Blotting made simple and easy

Only imager on the market to use lasers. Narrow, specific laser excitation leads to higher signal and increases the overall sensitivity of your Western blots.

Western Blotting Done Right

THE REACTION

– Image blots up to 420 DPI

THE IMAGER

– Features auto-focus, auto-light control, auto-image capture, and dynamic flat fielding

– Integrated touchscreen for ease of use and easy configuration with an external PC

– Ideal for fluorescent, chemiluminescent, ultraviolet, and visible imaging with a 4.8 OD range

– Equipped with high-resolution CCD cameras with fast lens options, adjustable optical and lens settings, adjustable height tray with auto-detection, and pre-calibrated focus

THE DATA

Journal of Proteomics Tubulin or Not Tubulin: Heading Toward Total Protein Staining as Loading Control in Western Blots

“Total protein staining (TPS) represents the actual loading amount more accurately than HKPs due to minor technical and biological variation. Further, the broad dynamic range of TPS solves the issue of HKPs that commonly fail to show loading differences above small loading amounts of 0.5-10 μg.”

Moritz CP. Tubulin or Not Tubulin: Heading Toward Total Protein Staining as Loading Control in Western Blots. Proteomics. 2017 Oct;17(20). doi: 10.1002/pmic.201600189. PMID: 28941183

Time and Cost Savings with Fluorescent Western Blotting*

ECL WBECL WBFLUORESCENCE WB
Detection MethodFilmCCD ImagerFluorescence Imager
# of Target Proteins2 Targets2 Targets2 Targets
Protein Markers (0.5-1uL for IR; 10uL for Chemi)$2.84$2.84$0.28
Secondary Antibody (1:10,000 for FL ; 1:20,000 for ECL)$0.20$0.20$0.68
ECL Substrate (0.1 ml/cm2)$32.80$32.80$0.00
Photographic Film (2-4 sheets/ membrane)$9.96$0.00$0.00
Stripping Buffer (20 ml/reaction)$5.06$5.06$0.00
Total Experiment Cost$50.86$40.90$0.96
Labor hours8.3 Hours per WB7.4 Hours per WB4.3 hours per WB

Western Blot expenses based on the use of consumables. Calculations assume a 10x10cm Western Blot. Online prices, February 2021.

Laser-based NIR Imaging

Unique near-infrared (NIR) lasers help keep signal high and background low. Our high-performance multiplex NIR lasers and filters deliver robust excitation energy, which maximizes emission strength for maximum sensitivity.

Fluorescent Western Blot of STAT1 and phospho-STAT1

Multi-Channel Detection Capabilities

Harness the power to do multiple applications with one imager!

The Azure Imaging Systems’ laser technology offers two NIR detection channels along with three visible fluorescence (RGB) fluorescence channels. This allows you to study multiple proteins in a single assay, even if those targets overlap in molecular weight. You can also easily resolve and quantify co-migrating bands, such as phosphorylated versus pan-protein forms.

Product Specifications:
  • Focus-free imaging, no-positioning necessary
  • One-touch workflows—select your application and the instrument chooses the appropriate light source and filters
  • AzureSpot Analysis Software for powerful and streamlined quantitative analysis
  • Optional: Two NIR lasers (660 nm and 785 nm) that keep signal high and background low
  • Optional: Efficient multiplex detection with three visible fluorescence channels and/or two NIR fluorescence channels
  • Optional: Q-module that adds the capability to perform total protein normalization using AzureRed Total Protein Stain

Azure IMAGING SYSTEMS

Innovative Design Translates to Better Performance

AZURE SAPPHIRE ™LI-COR ODYSSEY® CLX®AMERSHAM® TYPHOON®
Lens-Sample DistanceShortestLong (reflecting mirrors)
Visible Fluorescence
Chemiluminescence
Phosphor Imaging
Densitometry
Resolution10µm21µm10µm
Price$$$$

Quantitative Confidence with Fluorescent Western Blotting

Quantitative Western blots require the normalization of signal to an internal loading control. Guidelines from leading journals state that total protein normalization is preferred over normalization to housekeeping proteins.

  • Utilize up to three channels for proteins of interest and the fourth for an internal loading control
  • Experience no crosstalk between fluorescent channels
  • Easily identify proteins with small shifts in molecular weight (ie. post-translational modifications), without stripping and reprobing
Publications

Documents

Document TypeDescription
BrochureSapphire Biomolecular ImagerDOWNLOAD
BrochureHow Do You View-InfographicDOWNLOAD
BrochureSapphire Applications OverviewDOWNLOAD
Application NoteThree-Color Western Blots with AzureSpectra AntibodiesDOWNLOAD
Application NoteImaging Three-color Western Blots with the Azure c600DOWNLOAD
Application NoteAccurate Western Blot Normalization with AzureRed Fluorescent Protein StainDOWNLOAD
Application NotePhosphorylated Protein Detection is More Efficient by Fluorescent Western BlotDOWNLOAD
Application NoteIncreasing Assay Efficiency with Four Color DetectionDOWNLOAD

Videos

Azure Sapphire Video

pERK Detection via ICW Video

Shopping cart
There are no products in the cart!
Continue shopping