
The evolution of the ELISA and other immunoassays
If you’re reading this blog there’s a high chance that you use antibodies in your work, in some form of immunoassay. However, many of us
The Azure Imaging Systems has laser technology that offers two near-infrared (NIR) detection channels making it possible to study more than one protein in an assay, even if those targets overlap in molecular weight.
Easily resolve and quantify co-migrating bands, such as phosphorylated and nonphosphorylated isoforms of a protein, or isoforms containing other post-translational modifications. Fluorescent detection provides a larger linear dynamic range than chemiluminescent detection for more accurate quantitation. Imaging with NIR dyes also offers signal stability. An advantage of NIR fluorescent detection over visible fluorescent detection is minimal background because materials including tissue culture plastics demonstrate less autofluorescence in the NIR spectrum.
Fluorescent detection provides a larger linear dynamic range than chemiluminescent detection for more accurate quantitation. An advantage of NIR fluorescent detection over visible fluorescent detection is minimal background because materials including tissue culture plastics demonstrate less autofluorescence in the NIR spectrum. Imaging with NIR dyes also offers signal stability.
NIR detection is available on the Azure 500 Imager, as well as the Azure Sapphire Biomolecular Imager.
Next generation laser-scanning system with unmatched flexibility and performance.
The imagers that are revolutionizing Western blot imaging.
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If you’re reading this blog there’s a high chance that you use antibodies in your work, in some form of immunoassay. However, many of us
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