Azure Biosystems
Near-infrared Western blots (NIR) allow you to easily resolve and quantify co-migrating bands, such as phosphorylated and nonphosphorylated isoforms of a protein, or isoforms containing other post-translational modifications. Fluorescent detection provides a larger linear dynamic range than chemiluminescent detection for more accurate quantitation. Imaging with NIR dyes also offers signal stability.
One advantage of NIR fluorescent detection over visible fluorescent detection is minimal background because materials including tissue culture plastics demonstrate less autofluorescence in the NIR spectrum.
Fluorescent detection provides a larger linear dynamic range than chemiluminescent detection for more accurate quantitation.
An advantage of near-infrared (NIR) fluorescent detection over visible fluorescent detection is minimal background. This is because various materials, including tissue culture plastics, demonstrate less autofluorescence in the NIR spectrum. Imaging with NIR dyes also offers signal stability.
The Azure Imaging Systems have laser technology that offers two near-infrared (NIR) detection channels, which make it possible to study more than one protein in an assay, even if those targets overlap in molecular weight.
NIR detection is available on the Azure 500 Imager, as well as the new Azure Sapphire FL Biomolecular Imager.
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