Multiplexing fluorescent Western blots, also referred to as multiplex fluorescence, is the ability to detect multiple proteins simultaneously on the same blot. This allows you to get all the information you need at once. Fluorescent Western blotting uses secondary antibodies directly conjugated to fluorescent dyes.
Fluorophores for fluorescent Western blotting can be chosen based on their specific excitation and emission spectra, enabling multiplexing (Figure 1) for faster, more efficient studies. One of the biggest advantages of fluorescent detection versus chemiluminescence is the ability to use more than one antibody per assay (see the Table).