Membrane Arrays

Membrane arrays offer a multiplex approach to assaying protein expression. They are membrane-based assays that allow simultaneous screening of multiple proteins in a sample. Membrane antibody arrays are commercially available from many suppliers and can be constructed to assess less than 10 to hundreds of proteins in a pathway or biological system of interest. The arrays are convenient for assaying expression of proteins involved in inflammation, angiogenesis, apoptosis, cell signaling, and other cellular processes.

A membrane antibody array is a sandwich-style immunoassay, similar to an ELISA. The difference between the two is that the capture antibody is spotted on a membrane, instead of the bottom of the well of a dish for ELISA. The sample is applied to the membrane and any proteins that become bound to the capture antibodies are detected with a second set of labeled antibodies. The arrays are usually imaged using chemiluminescent signal.