Gel Documentation

Imaging DNA and Protein Gels

UV Imaging using Ethidium Bromide

Nucleic acid and protein gels are a fundamental part of lab life. DNA or RNA is usually stained by ethidium bromide, which intercalates into the nucleic acids and can be visualized with UV light. Safer dyes for staining DNA (SYBR® Safe, SYBR® Gold, SYBR® Green) are also growing in popularity, and can imaged with blue light.
Protein purification procedures often rely on verifying purity by SDS-PAGE and subsequent gel staining (Coomassie blue or silver stain) to check for contaminating protein bands.
The Azure 200 imager has the imaging capabilities for all of these applications. With a dual-wavelength 302 nm and 365 nm UV transilluminator, ethidium bromide-stained DNA gels can be imaged in a fraction of a second. For excising bands from a gel, the UV transilluminator platform can be pulled out.

The Azure 400 and Azure 600 imagers, as well as the Azure Sapphire Biomolecular Imager, open up the ability to document protein gels stained with a wide variety of fluorescent protein stains that fluoresce in the visible spectrum. 

Many stains, including Azure TotalStain Q, AzureRed, Coomassie Fluor™, Deep Purple™, SYPRO® Ruby, SYPRO® Red, SYPRO® Tangerine, and more can be imaged with advanced imagers, such as the Azure Imaging Systems.

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