Azure Biosystems
Reagent Samples

TotalStain Q Samples

Which Total Protein Stain is right for you?

Total Protein StainFeatures
TotalStain Q PVDF
  • For PVDF membranes
  • Linear at high protein loads (1-50μg per lane)
  • Compatible with both fluorescent and chemiluminescent WB
  • Versatile: can be imaged with CCD-based imagers and scanners with green channel or Safe-dyes channel
  • Convenient: 20-minute protocol. No destaining required
TotalStain Q NC
  • For Nitrocellulose membranes
  • Linear at high protein loads (1-50μg per lane)
  • Compatible with both fluorescent and chemiluminescent WB
  • Versatile: can be imaged with CCD-based imagers and scanners with green channel or Safe-dyes channel
  • Convenient: 24-minute protocol. No destaining required

Which substrate is right for you?

SubstrateFeatures
Radiance ECL™ Substrate
  • Economical
  • linear signal for Quantitative Westerns
Radiance Q™ Substrate
  • Higher linear dynamic range for more reliable quantitation
  • Increased sensitivity
  • Extended signal longevity
Radiance™ Plus Substrate
  • Ultra-high sensitivity for low abundance proteins

Which blocking buffer is right for your assay?

Blocking BufferFeatures
Chemi Blot Blocking Buffer
  • lowers background and enhances signal
  • formulated specifically for chemiluminescence
Fluorescent Blot Blocking Buffer
  • stabilizes antibody interaction
  • lowers background and enhances signal
  • formulated specifically for fluorescence
Protein-Free Blot Blocking Buffer
  • stabilizes antibody interaction
  • great for antibodies with a high level of cross reactivity with protein-based blockers
  • compatible with both chemiluminescence and fluorescence

TRANSFER BUFFER SAMPLES

Enhanced protein transfer for improved sensitivity

Azure’s proprietary transfer buffer provides improved detection of low-abundance and post-translationally modified proteins. High-efficiency protein transfer and increased protein retention on the membrane add up to more sensitive Western blots.


Provided as a 50x concentrated solution

New to fluorescent Western blotting?

Azure’s Fluorescent Demo Kits contain everything you need to perform a single color fluorescent Western using your sample and primary antibodies.

Email info@azurebiosystems.com to request a demo kit.

Single use demo kit for the Azure Flash Western. 

Email info@azurebiosystems.com to request a demo kit.

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