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Western Blotting
Reagent Samples

Looking for the right Western blotting reagents? You've come to the right spot!

Azure Biosystems has a full range of transfer buffers, substrates, and blocking buffers to support your Western blot workflow. You can request a free sample using the form this page. Visit our online store to browse full size options.

Which Total Protein Stain is right for you?

TotalStain Q PVDF

• For PVDF membranes

• Linear at high protein loads (1-50μg per lane)

• Compatible with both fluorescent and chemiluminescent WB

• Versatile: can be imaged with CCD-based imagers and scanners with green channel or Safe-dyes channel

• Convenient: 20-minute protocol. No destaining required

TotalStain Q NC

• For Nitrocellulose membranes

• Linear at high protein loads (1-50μg per lane)

• Compatible with both fluorescent and chemiluminescent Western blots

• Versatile: can be imaged with CCD-based imagers and scanners with green channel or Safe-dyes channel

• Convenient: 24-minute protocol. No destaining required

choose a Total Stain

Which substrate is right for you?

Radiance ECL™ Substrate

• Economical

• Linear signal for Quantitative Westerns

Radiance Q™ Substrate

• Higher linear dynamic range for more reliable quantitation

• Increased sensitivity

• Extended signal longevity

Radiance™ Plus Substrate

• Ultra-high sensitivity for low abundance proteins

Choose a Substrate

Which blocking buffer is right for your assay?

Chemi Blot Blocking Buffer

• Lowers background and enhances signal

• Formulated specifically for chemiluminescent detection

Fluorescent Blot Blocking Buffer

• Stabilizes antibody interaction

• Lowers background and enhances signal

• Formulated specifically for fluorescent detection

Protein-Free Blot Blocking Buffer

• Stabilizes antibody interaction

• Great for antibodies with a high level of cross reactivity with protein-based blockers

• Compatible with both chemiluminescence and fluorescent detection>

Choose a Blocking Buffer

TRANSFER BUFFER

Enhanced protein transfer for improved sensitivity

Azure’s proprietary transfer buffer provides improved detection of low-abundance and post-translationally modified proteins. High-efficiency protein transfer and increased protein retention on the membrane add up to more sensitive Western blots.


Provided as a 50x concentrated solution

New to fluorescent Western blotting?

Azure’s Fluorescent Demo Kits contain everything you need to perform a single color fluorescent Western using your sample and primary antibodies.

Email info@azurebiosystems.com to request a free demo kit.

Flash Western Chemiluminescence Kit

Single use demo kit for the Azure Flash Western. 

Email info@azurebiosystems.com to request a free demo kit.


Azure Biosystems
6747 Sierra Court, Suite A-B
Dublin, CA 94568
United States

Tel: (925) 307 7127
Fax: (925) 905-1816
Info@azurebiosystems.com

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