Azure BiosystemsHigh-quality and affordable imaging systems, qPCR systems, and reagents.
Azure Biosystems
Designed for flexible choice in detection chemistry and samples, the Sapphire FL brings precise quantitation of nucleic acids and proteins
Flexibility with uncompromising performance – From in vitro molecular assays to in vivo imaging
The Sapphire FL is the ultimate biomolecular imager for FLEXIBILITY. With customizable and user-changeable laser and filter modules, the Sapphire FL easily adapts to a lab’s changing needs and advancing research. The Sapphire FL offers customizable and user-changeable optical modules, 5–1000 μm resolution scans, a Z-plane range from -1.0 to +6 mm, 5 anesthesia ports for imaging living animals, chemiluminescence detection through the Chemiluminescence Module, and much more. The new Azure Sapphire FL is the second generation of the ground breaking Azure Sapphire Imager.
The Sapphire FL features a unique, patent-pending design of interchangeable and customizable laser and filter modules, enabling a virtually infinite number of spectral combinations. A broad range of excitation and emission wavelengths, as well as phosphor imaging, are supported.
This versatility permits research-driven, rather than instrument-dictated, experimental design. With the Sapphire FL, the user-exchangeable optical modules (consisting of one laser and one emission filter) ensure there is no need to compromise.
The flexibility of the Sapphire FL guarantees researchers the freedom to confidently choose the best dyes and chemistry for any experiment. Custom optical modules can excite any dye at its peak, resulting in the highest possible signal for any fluorophore.
In addition to fluorescence, the Sapphire FL also excels at imaging phosphor screens and chemiluminescence using the optional Chemiluminescence Module.
No other system on the market matches the Sapphire FL’s coverage across the fluorescent spectrum. Extend your reach into the UV spectrum with our 375 custom optical module. High sensitivity allows femtogram detection of proteins labeled with common fluorescent dyes.
Extended dynamic range, when selected, allows imaging of both bright and weak bands without experiencing saturation. This is ideal for samples that feature strong and weak expressing proteins.
EDR extends dynamic range to 24 bits of data.
The Sapphire FL Z-plane ranges from -1 mm below to +6 mm above the glass imaging surface, allowing crystal clear imaging of samples with depth. The adjustable focal plane enables scanning of thick samples. Screen slides before microscopic analysis by imaging multiple slides at a resolution of 5 microns.
Optimize the focal plane for your sample. Easily switch between sample types with confidence that each image will be sharply in focus. Create Z-stacks and GIFs to visualize multiple focal planes at once.
We understand some samples are on the glass (Western blots), while some samples are raised above the glass surface (96-well plates). Some samples also have multiple focal points within them, such as tissue slides and organs. Sapphire FL can image those too.
The Sapphire FL offers a 7mm software-controlled adjustable focal plane, so you can scan your sample at multiple depths to ensure you see all your data has to offer.
The Chemiluminescence Module adds to the power of the Sapphire FL by providing high-resolution, quantitative chemiluminescence and visible imaging.
The new Sapphire FL provides you with the versatility to do more applications with one system.
| Parameter | Azure Sapphire FL |
|---|---|
| Detection modes | Fluorescence, phosphor imaging, densitometry, and chemiluminescence* |
| Detectors | Confocal scanning detected with PMT and APD lasers |
| Pixel size | 5, 10, 25, 50, 100, 200, 500, 1000 μm |
| Supported wavelength range | 375 – 900 nm |
| Excitation range | 375 – 850 nm |
| Emission range | 380 – 900 nm |
| Maximum field of view (FOV): | 25 cm x 25 cm |
| Z-plane focal range | 7 mm; minimum delta 0.01mm, from -1 mm below the glass to 6 mm above the glass |
| Standard optical modules | Solid-state laser diodes emitting at 488, 532, 638, 685, 784 |
| Custom optical modules** | Solid-state laser diodes emitting at 375, 450, 488, 532 |
| Wide dynamic range | 24-bit |
| Clearance from platen to lid | 4 cm |
| Animal imaging | Yes, with 5 built-in anesthesia ports compatible with standard small animal anesthesia tubing |
| Unit dimensions (W x H x D) | 63.0 cm x 39.9 cm x 59.3 cm / 24.8” x 15.7” x 23.3" |
| Weight | 43.5 kg / 95.9 lbs |
| Power requirements | 100 – 240 VAC ± 10%, 50/60 Hz |
| Connection to computer | Fast LAN (Ethernet) cable |
| Certifications | CE, cTUVus, CB scheme |
*Optional
**Custom lasers and filters are available upon request to provide exceptional research flexibility
Azure Imagers have been published in over 2,000 publications worldwide. Our high-resolution CCD cameras are the reason behind reproducible data and publication-ready images.
