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NEW! Azure Sapphire FL Biomolecular Imager

Designed for flexible choice in detection chemistry and samples, the Sapphire FL brings precise quantitation of nucleic acids and proteins

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Unmatched Application Versatility and Performance

laser-based imaging

24-bit Dynamic Range

 

Extended dynamic range allows imaging of both bright and weak bands without experiencing saturation.

5 micron resolution icon

5 Micron Resolution

 

From Western blots and gels, to microscope slides and live animal imaging, the adjustable 5-1000 µm resolution ensures unparalleled sample and application flexibility.

quantity accuracy

Custom Design Lasers and Filters

 

No limits – easily swap lasers and filters to achieve the perfect system to image your sample

Adjustable focus Sapphire FL

Adjustable focus for sample thickness

 

Whether imaging Western blots, gels, 96-well plates, or tissue slides, the adjustable Z-plane ensures optimal focus for your sample.

Key Features

Flexibility with uncompromising performance – From in vitro molecular assays to in vivo imaging

The Sapphire FL is the ultimate biomolecular imager for FLEXIBILITY. With customizable and user-changeable laser and filter modules, the Sapphire FL easily adapts to a lab’s changing needs and advancing research. The Sapphire FL offers customizable and user-changeable optical modules, 5–1000 μm resolution scans, a Z-plane range from -1.0 to +6 mm, 5 anesthesia ports for imaging living animals, chemiluminescence detection through the Chemiluminescence Module, and much more. The new Azure Sapphire FL is the second generation of the ground breaking Azure Sapphire Imager.

Unlimited Laser and Filter Wavelength combinations

The Sapphire FL features a unique, patent-pending design of interchangeable and customizable laser and filter modules, enabling a virtually infinite number of spectral combinations. A broad range of excitation and emission wavelengths, as well as phosphor imaging, are supported.

Scientist changing optical modules on the new Azure Sapphire FL
Pick the modules that support your research. Changing the optical modules on the new Sapphire FL is simple and easy. The unique mechanism makes selecting lasers to match your dyes finally possible.
Removing optical module from Sapphire FL
1. The new Sapphire FL gives you the freedom to choose the best laser combination for your studies. Begin by removing the optical module from Sapphire FL.
2. Remove and replace filter.
2. Remove and replace filter.
How to load new laser and filter combination into the Sapphire FL.
3. Load new laser and filter combination into the Sapphire FL.
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Sapphire FL Applications

Application Flexibility

The new Azure Sapphire FL supports imaging of Western blots, gels, phosphor screens, tissues, slides, arrays, and much more.

Sensitive Fluorescent Detection

High sensitivity allows femtogram detection of proteins labeled with common fluorescent dyes.

Bovine serum albumin (BSA) conjugated to AzureSpectra dyes, separated by SDS-PAGE, and transferred to membranes.
Bovine serum albumin (BSA) conjugated to AzureSpectra dyes, separated by SDS-PAGE, and transferred to membranes. Western blots were imaged at 50 μm on the Sapphire FL. Loaded amounts of dye-conjugated-BSA are given.
Scanning with EDR demonstrates quantifiable linearity over the entire range of sample concentrations.
A dot blot was scanned with (top image) and without (bottom image) EDR. Without EDR, the top four dots saturate and cannot be quantified. Scanning with EDR demonstrates quantifiable linearity over the entire range of sample concentrations.

Distinguish between Subtle Differences in Expression with Extended Dynamic Range (EDR)

Extended dynamic range, when selected, allows imaging of both bright and weak bands without experiencing saturation. This is ideal for samples that feature strong and weak expressing proteins.

EDR extends dynamic range to 24 bits of data.

5 Micron Resolution

Screen slides before microscopic analysis by imaging multiple slides at a resolution of 5 microns. The adjustable focal plane enables scanning of thick samples.

Mouse lung tissue imaged on the Azure Sapphire FL
Mouse lung tissue slide probed for vascular endothelial (VE)-cadherin (AzureSpectra 550 nm secondary antibodies) and smooth muscle actin (SMA) (AzureSpectra 650 nm secondary antibodies). Imaged on the Sapphire FL using the 532 and 638 standard optical modules (red and green, respectively) at 5 um.
Mouse lung tissue slide imaged on the Sapphire FL with 638 nm laser modules at 5 μm.
Mouse lung tissue slide stained with 650-Smooth Muscle Actin (SMA). Imaged on the Sapphire FL with 638 nm laser modules at 5 μm.
A 50-micron scan of a flower was taken using an Azure Sapphire FL from 0 to 6 mm with 1 mm adjustments.
A. 50-micron scan of a flower was taken from 0 to 6 mm with 1 mm adjustments.
50-micron scan of the same flower was merged in the Sapphire FL Capture Software to view all areas of focus at once.
B. 50-micron scan of the same flower was merged in the Sapphire FL Capture Software to view all areas of focus at once.

Adjustable Focus

Some samples are on the glass (Western blots) while some samples are raised above the glass surface (96-well plates). Some samples have multiple focal points within them, such as tissue slides and organs. With a 7mm software controlled adjustable focal plane, you can scan your sample at multiple depths to ensure you can see all that your data has to offer. 

Chemiluminescent Imaging When You Need It

Upgrade to the Sapphire FL Chemiluminescence Module to add quantitative, reproducible chemiluminescent imaging to your studies.

chemiluminescent FLASH Western Blot HeLa serial each in duplicate antitubulin antiactin with marker imaged with Azure Biosystems chemiSOLO
Chemiluminescent FLASH Western Blot HeLa serial each in duplicate antitubulin antiactin with marker imaged with the Sapphire FL Chemiluminescent Module.

Applications

The new Sapphire FL provides you with the versatility to do more applications

Documents

Document TypeDescription
BrochureAzure Sapphire FL Biomolecular Imager BrochureDOWNLOAD

Sapphire FL Pricing

Contact us today for a quote and to learn more about how Azure is changing how researchers image blots, arrays, small organisms, and more.

Part NumberProduct Name
IS4000Sapphire FL Biomolecular ImagerGET PRICING

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