qPCR
Joy DeTorres March 25, 2026
Azure BiosystemsHigh-quality and affordable imaging systems, qPCR systems, and reagents.
Azure BiosystemsReal-time PCR detection measures DNA or RNA amplification as it happens, allowing researchers to quantify target sequences with high sensitivity, accuracy, and reproducibility. The Azure Cielo™ system supports streamlined qPCR workflows for applications ranging from gene expression to pathogen detection.
Fluorescent probe-based detection
Intercalating dye detection
Multiplex qPCR assays
Gene expression analysis
Genotyping studies
Pathogen detection
Library quantification
Multiplex target detection
The Azure Cielo™ Real-Time PCR system is designed to simplify qPCR setup, improve run-to-run consistency, and support sensitive multiplex detection. With intuitive software, flexible connectivity, robust thermal control, and high-performance optical detection, Cielo helps labs generate reliable qPCR data with less workflow friction.
Six-target multiplexing
Fast thermal cycling
High reproducibility
Smart protocol setup
Flexible data transfer
Wide dynamic range
Compare key features across imaging systems to find the best fit for your applications
| Parameter | Cielo 6 (AIQ060) | Cielo 3 (AIQ030) |
|---|---|---|
| Sample capacity (Wells) | 96 wells | 96 wells |
| Compatible Plates/Tubes | 96-well plates and tubes, 8- or 12-well strips and single tubes | 96-well plates and tubes, 8- or 12-well strips and single tubes |
| Reaction Volume | 1-50 uL (10-50 uL recommended) | 1-50 uL (10-50 uL recommended) |
| Dynamic Range | 10 Logs | 10 Logs |
| Excitation Source | LED Excitation range: 450-730 nm | LED Excitation range: 450-730 nm, 610-700 nm |
| Detection Channels | 6 | 3 |
| Multiplexing | Up to 6 targets | Up to 3 targets |
| Reaction Temperature | 4°C to 99.9°C | 4°C to 99.9°C |
| Max Block Ramp Rate | 6°C/sec | 6°C/sec |
| Average Sample Ramp Rate | 4°C/Sec | 4°C/Sec |
| Temperature Uniformity | ±0.2°C | ±0.2°C |
| Temperature Accuracy | ±0.1°C | ±0.1°C |
| Custom Dye/Chemistry | ✓ | ✓ |
| Onboard Memory | 32 GB Equivalent to 20,000 experiments | 32 GB Equivalent to 20,000 experiments |
| Unit dimensions (W x H x D) | 30.5 cm x 50.8 cm x 43.2 cm / 12” x 20” x 17" | 30.5 cm x 50.8 cm x 43.2 cm / 12” x 20” x 17" |
| Weight | 22.7kg / 50 lbs | 22.7kg / 50 lbs |
| Power requirements | 100-240 VAC, 8A; Consumption: 0.8kW (800 Watts) | 100-240 VAC, 8A; Consumption: 0.8kW (800 Watts) |
| Connectivity | USB, Wifi, Ethernet | USB, Wifi, Ethernet |
| Certifications | CE Certified, TUV Certified | CE Certified, TUV Certified |
| Dyes/Probes | Cielo 6 (AIQ060) | Cielo 3 (AIQ030) |
|---|---|---|
| • SYBR® Green • EvaGreen™ • FAM™ | ✓ | ✓ |
| • VIC® • HEX™ • JOE™ • CAL Fluor® 540 • CAL Fluor® Orange 560 | ✓ | ✓ |
| • TAMRA™ • Cy3 | ✓ | |
| • ROX™ • TEX®615, • CAL Fluor® Red 610 • CXR | ✓ | |
| • Cy®5 • Quasar®670, • Liz® • Mustang Purple® | ✓ | ✓ |
| • Cy®5.5 • Quasar 705 | ✓ |
Western blot detection is a common laboratory technique used to identify specific protein molecules within a complex mixture, such as tissue or cell lysate, based on their size and antibody binding.
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