Get a quick overview of the steps you can take to ensure your Western blots are quantitative. This free guide also includes a troubleshooting section and tear-out quantitative western blotting checklist.
Getting quantitative data from your western blot takes more than just a fluorescent secondary and digital imager. You need to consider your sample prep, your normalization method, and the linearity of your signal. To help keep your quantitative western blot workflows on track, we’ve created this checklist of steps that can be taken to ensure your western blot quantitation is as accurate as it can be.
Consistency is key!
The linearity of your signal can be affected by multiple factors. Here are steps you can take to ensure linearity and improve the accuracy of your quantitation:
With products that offer sensitive detection, wide dynamic range, easy-to-use features, and lot-to-lot consistency Azure Biosystems expertly streamlines and supports your quantitative fluorescent western blot workflows:
Powerful and flexible western blot imagers
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Reliable reagents that support accurate quantitation
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