Azure BiosystemsHigh-quality and affordable imaging systems, qPCR systems, and reagents.
Azure Biosystems
Large format scanner for precise quantitation of molecular assays
Flexibility with uncompromising performance
The Sapphire FL is the second generation Sapphire and ultimate biomolecular imager for FLEXIBILITY. With customizable and user-changeable laser and filter modules, it easily adapts to a lab’s changing needs and advancing research. The Sapphire FL offers customizable and user-changeable optical modules, 5–1000 μm resolution scans, a Z-plane range from -1.0 to +6 mm, five anesthesia ports for imaging living animals, chemiluminescence detection through a Chemiluminescence Module, and much more.
The Sapphire FL+ is one of only two scanners in its class that supports phosphor imaging—a highly sensitive assay that utilizes radioactivity. It can scan storage phosphor screens up to 20 x 40 cm, delivering outstanding dynamic range and image quality through its use of laser excitation and photon multiplier tube (PMT) detection.
Sapphire FL+ features a unique, patent-pending design of interchangeable and customizable laser and filter modules, enabling a virtually infinite number of spectral combinations. A broad range of excitation and emission wavelengths, as well as phosphor imaging, are supported.
Sapphire FL and Sapphire FL+ are the only biomolecular scanners that offer modular light sources, from the UV spectrum to the NIR. Using the 375 Standard Optical Module, it is able to image common DNA stains, such as Dapi and Hoechst.
High sensitivity allows femtogram detection of proteins labeled with common fluorescent dyes.
Extended dynamic range allows you to image both bright and weak bands without experiencing saturation. This is ideal for the samples that feature strong and weak expressing proteins.
EDR extends dynamic range up to 24 bits of data.
Some samples can be imaged on the glass (Western blots), while others are raised above the glass surface (96-well plates). The Sapphire FL+ can image them all.
Some samples have multiple focal points within them, such as tissue slides and organs. The Sapphire FL+ can image those too.
It offers a 7mm software-controlled adjustable focal plane, so you can scan your sample at multiple depths to ensure you see all your data has to offer.
The Sapphire FL+ scans between 5 – 1000 µm resolution to ensure clear visualization of detailed samples. Image complex and minute samples, including tissue and microarray slides, with accuracy. Triage multiple slides at high resolution before microscopic analysis.
An optional Chemiluminescence Module adds high-resolution, quantitative chemiluminescence and visible imaging.
It allows you to capture proteins with femtogram sensitivity and gives you the ability to capture color marker images.
The new Sapphire FL+ provides you with the versatility to do more applications with one system. Browse the list of published examples below to see what the Sapphire FL+ is capable of.
| Parameter | Sapphire FL+ |
|---|---|
| Detection modes | Fluorescence, phosphor imaging, densitometry, and chemiluminescence* |
| Detectors | Confocal scanning with lasers and PMT or APD detectors |
| Pixel size | 5, 10, 20, 25, 50, 75, 100, 150, 170, 200, 500, 1000 μm |
| Supported wavelength range | 375 – 900 nm |
| Excitation range | 375 – 850 nm |
| Emission range | 380 – 900 nm |
| Maximum field of view (FOV): | 46 cm x 25 cm |
| Z-plane focal range | 7 mm; minimum delta 0.01mm, from -1 mm below the glass to 6 mm above the glass |
| Standard optical modules | Solid-state laser diodes emitting at 375, 450, 488, 532, 638, 658, 685, 730, and 784 nm. Phosphor Standard Optical Module available. |
| Custom Optical Modules | Custom lasers and filters are available upon request to provide exceptional research flexibility. |
| Wide dynamic range | 24-bit |
| Clearance from platen to lid | 5 cm |
| Unit dimensions (W x H x D) | 96.8 x 66 x 40.7 cm (38.1 x 26 x 16 in) |
| Weight | 71 kg / 156.5 lbs |
| Power requirements | 100 – 240 VAC ± 10%, 50/60 Hz |
| Connection to computer | Fast LAN (Ethernet) cable |
| Certifications | CE, RoHS, cTUVus, CSA, CB scheme |
*Chemiluminescence module sold separately
| Title | Link |
|---|---|
Our lab recently acquired a Sapphire FL Biomolecular Imager. This imager is a game-changer, and the analysis software is fantastic. Going from regularly using an imager built in the 90’s to a unit this modern is a substantial leap forward, but this overdue upgrade in machinery is not the sole reason for our excitement. Its sensitivity, resolution, and user-friendly UI speak volumes for the quality of the product and has made imaging an even more enjoyable part of our experiments. My colleagues and I are looking forward to using it for… more
For Research Use Only
