Our lab recently acquired a Sapphire FL Biomolecular Imager. This imager is a game-changer, and the analysis software is fantastic. Going from regularly using an imager built in the 90’s to a unit this modern is a substantial leap forward, but this overdue upgrade in machinery is not the sole reason for our excitement. Its sensitivity, resolution, and user-friendly UI speak volumes for the quality of the product and has made imaging an even more enjoyable part of our experiments. My colleagues and I are looking forward to using it for years to come… more
Extended dynamic range allows imaging of both bright and weak bands without experiencing saturation.
From Western blots and gels, to microscope slides and live animal imaging, the adjustable 5-1000 µm resolution ensures unparalleled sample and application flexibility.
No limits – easily swap lasers and filters to achieve the perfect system to image your sample
Whether imaging Western blots, gels, 96-well plates, or tissue slides, the adjustable Z-plane ensures optimal focus for your sample.
Flexibility with uncompromising performance – From in vitro molecular assays to in vivo imaging
The Sapphire FL is the ultimate biomolecular imager for FLEXIBILITY. With customizable and user-changeable laser and filter modules, the Sapphire FL easily adapts to a lab’s changing needs and advancing research. It offers customizable and user-changeable optical modules, 5–1000 μm resolution scans, a Z-plane range from -1.0 to +6 mm, 5 anesthesia ports for imaging living animals, chemiluminescence detection through the Chemiluminescence Module, and much more. The new Azure Sapphire FL is the second generation of the ground breaking Azure Sapphire Imager.
The Sapphire FL features a unique, patent-pending design of interchangeable and customizable laser and filter modules, enabling a virtually infinite number of spectral combinations. A broad range of excitation and emission wavelengths, as well as phosphor imaging, are supported.
This versatility permits research-driven, rather than instrument-dictated, experimental design. With the Sapphire FL, the user-exchangeable optical modules (consisting of one laser and one emission filter) ensure there is no need to compromise.
The flexibility of the Sapphire FL guarantees researchers the freedom to confidently choose the best dyes and chemistry for any experiment. Custom optical modules can excite any dye at its peak, resulting in the highest possible signal for any fluorophore.
In addition to fluorescence, the Sapphire FL also excels at imaging phosphor screens and chemiluminescence using the optional Chemiluminescence Module.
Several attributes of the Sapphire FL imager make imaging of live animals possible. When combined with appropriate tubing and nose cones, living mice can be imaged under anesthesia using one of five built-in anesthesia ports.
The wide imaging platform can accommodate specimens with depth (up to 4 cm) allowing mice, plants, petri dishes, and other large biological samples to be imaged.
Extended dynamic range, when selected, allows imaging of both bright and weak bands without experiencing saturation. This is ideal for samples that feature strong and weak expressing proteins.
EDR extends dynamic range to 24 bits of data.
High sensitivity allows femtogram detection of proteins labeled with common fluorescent dyes.
No other system on the market matches the Sapphire FL’s coverage across the fluorescent spectrum. Extend your reach into the UV spectrum with our 375 Standard Optical Module.
We understand some samples are on the glass (Western blots), while some samples are raised above the glass surface (96-well plates). Some samples also have multiple focal points within them, such as tissue slides and organs. Sapphire FL can image those too.
The Sapphire FL offers a 7mm software-controlled adjustable focal plane, so you can scan your sample at multiple depths to ensure you see all your data has to offer.
The Sapphire FL Z-plane ranges from -1 mm below to +6 mm above the glass imaging surface, allowing crystal clear imaging of samples with depth. The adjustable focal plane enables scanning of thick samples. Screen slides before microscopic analysis by imaging multiple slides at a resolution of 5 microns.
Optimize the focal plane for your sample. Easily switch between sample types with confidence that each image will be sharply in focus. Create Z-stacks and GIFs to visualize multiple focal planes at once.
The Chemiluminescence Module adds high-resolution, quantitative chemiluminescence and visible imaging.
The chemi module allows you to express proteins with femtogram sensitivity and gives you the ability to capture marker images.
The new Sapphire FL provides you with the versatility to do more applications with one system. Browse the list of published examples below to see what the SFL is capable of.
Sascha D. Markowitsch, Kira M. Juetter, Patricia Schupp, Kristine Hauschulte, Olesya Vakhrusheva, Kimberly Sue Slade, Anita Thomas, Igor Tsaur, Jindrich Cinatl, Jr., Martin Michaelis, Thomas Efferth, Axel Haferkamp and Eva Juengel
Wioletta Rut, Mikolaj Zmudzinski, Scott J. Snipas, Miklos Bekes, Tony T. Huang and Marcin Drag
…Biotinylated Ub-based probes were detected with a fluorescent streptavidin Alexa Fluor 647 conjugate (1 : 10 000) in TBS-T with 1% BSA, and UCH-L3 was detected with a mouse antihuman monoclonal IgG1 antibody (1 : 1000) and fluorescent goat anti-mouse (1 : 10 000) using an Azure Biosystems Sapphire Biomolecular Imager and Azure Spot Analysis Software.
Luís A. Rocha, Eduardo D. Gomes, João L. Afonso, Sara Granja, Fatima Baltazar, Nuno A. Silva, Molly S. Shoichet, Rui A. Sousa, David A. Learmonth and Antonio J. Salgado
…The evaluation of the angiogenic and neurotrophic profile of the previously obtained secretomes was performed using the Human Neuro Discovery Array C1 and Human Angiogenesis Array C1 (RayBiotech, United States)…Finally, the chemiluminescence image of each membrane was obtained using a Sapphire Biomolecular Imager (Azure Biosystems, United States). The intensity of each dot was quantified using the AzureSpot software (Azure Biosystems, United States)…
Byung Ha Lee, Rui Wang, Ingrid M. Moberg, Sarah H. Reeder, Prativa Amom, Michelle H. Tan, Katelyn Amstutz, Pallavi Chandna, Adam Helton, Ekaterina P. Andrianova, Igor B. Zhulin, and Anna A. Dobritsa
Agrobacteria were collected and resuspended in infiltration buffer (10 mM MgCl2, 10 mM MES, 150 μM acetosyringone) at a final concentration of OD600 = 0.8. Pairwise combinations of suspensions were infiltrated into young tobacco leaves, which were then allowed to grow for 3 d in light. A total 12–16 leaves were collected from five to ten plants, the abaxial side of leaves was sprayed with 1 mM luciferin (Biosynth, L-8220) and kept in the dark at 4 °C for 30 min. The bioluminescence images were captured using Azure Sapphire Biomolecule Imager (Azure Biosystems) and converted to heatmaps using the 16-colour look-up table from ImageJ v.1.53a…
Katerina Bakela, Maria Georgia Dimitraki, Evangelia Skoufa, Irene Athanassakis
…Liver tissues were isolated 6 months after the ConA and the ConA + sMHCII treatment of mice and fixed using PFA 4%, under rotation, at 4°C for 24 hours. Azure Biosystems Sapphire™ Biomolecular Imager (Azure Biosystems, Dublin, CA 94568 USA) was used in order to scan the liver tissues in 10-μm resolution. This instrument combines NIR fluorescence (both long and short), RGB fluorescence, chemiluminescent, and phosphor imaging capabilities, while using four solid state lasers as excitation sources (450, 520, 660, and 780 nm). The application of the four-channel fluorescence mode at 10-μm resolution, could detect gross anatomy and morphology of liver tissues, mainly based on tissue autofluorescence…
Mattern KMJ, Blancas–Velázquez AS, Ngo MT, et al.
Cryostat sections of 12-μm thickness were mounted on slides and blocked in 5% normal donkey serum (Abcam). Immunohistochemistry was performed as previously described…Sections were incubated in primary antibody at 4°C overnight and in secondary antibody at room temperature for…1 h if used for whole section imaging…Images of whole sections were taken on an Azure Sapphire Biomolecular Imager (Azure Biosystems). Fluorescence signal intensity after background substraction [sic] was quantified with the Azure Spot software… “Western blot” for image acquisition, an Azure Sapphire Biomolecular Imager was used. The density of bands was quantified with the Azure Spot software.
laser scanning, ISL2 was detected using a sheep anti-hlslet-2 primary antibody and an Alexa Fluor™ 790 donkey anti-sheep secondary antibody. For Western blotting, ISL2 was detected with a sheep-anti-human Islet-2 antibody and a donkey-anti-sheep Alexa Fluor™ 680 secondary antibody. ACTB was detected with a rabbit-anti-beta actin antibody and a donkey-anti-rabbit Alexa Fluor™ 790 secondary antibody.
Sylwia Modrzycka, Sonia Kołt, Stéphanie G. I. Polderdijk, Ty E. Adams, Stanisław Potoczek, James A. Huntington, Paulina Kasperkiewicz and Marcin Drąg
…For the simultaneous coagulation factor labeling, human plasma was incubated with 5 μM of each fluorescently labeled probe…and then 5 μL of each sample was run onto a 10% MES (w/v) 15-well gel…The gel was then directly scanned at 520 nm for Cy3, 658 nm for Cy5, and 784 nm for Cy7 detection using an Azure Biosystems Sapphire Biomolecular Imager and Azure Spot Analysis Software.
Lena Landaverde, Winnie Wong, Gabriela Hernandez, Andy Fan & Catherine Klapperich
…To identify 5′-FAM-specific LAMP products, unstained agarose and acrylamide gels were imaged under 488-nm laser excitation using the Sapphire Biomolecular Imager…
Muhammad Mujammami, Mohamed Rafiullah, Assim A. Alfadda, Khalid Akkour, Ibrahim O. Alanazi, Afshan Masood, Mohthash Musambil, Hani Alhalal, Maria Arafah, Anas M. Abdel Rahman and Hicham Benabdelkamel
…The gels were scanned with Sapphire Biomolecular Imager (Azure Biosystems…) and digitalized via the image analysis software Sapphire Capture system (Azure Biosystems…
Brian J. Caldwell, Andrew S. Norris, Caroline F. Karbowski, Alyssa M. Wiegand, Vicki H. Wysocki & Charles E. Bell
…A gel shift DNA binding assay used two complementary 50-mer oligonucleotides labeled at the 5′-end with either Cy3 or Cy5…For visualization 17.5 μl of each complex was mixed with 7.5 μl Orange G dye…Gels were imaged using a Sapphire Biomolecular Imager (Azure Biosystems) with Sapphire Capture Software (version 1.12.0921.0). Scanning parameters for Fig. 8 were pixel size 100 μm, scan speed high, 2.38mm focus, intensity 2 for Cy5, intensity 4 for Cy3, black lighting 50, white 37186, gamma 1.37. Scanning parameters for Supplementary Fig. 1a, b were intensity 1 for Cy5, intensity 2 for Cy3, black lighting 50, white 15362, gamma 0.88.
Jana Grune, Andrew J. M. Lewis, Masahiro Yamazoe, Maarten Hulsmans, David Rohde, Ling Xiao, Shuang Zhang, Christiane Ott, David M. Calcagno, Yirong Zhou, Kerstin Timm, Mayooran Shanmuganathan, Fadi E. Pulous, Maximilian J. Schloss, Brody H. Foy, Diane Capen, Claudio Vinegoni, Gregory R. Wojtkiewicz, Yoshiko Iwamoto, Tilman Grune, Dennis Brown, John Higgins, Vanessa M. Ferreira, Neil Herring, Keith M. Channon, Stefan Neubauer, Oxford Acute Myocardial Infarction (OxAMI) Study,* David E. Sosnovik, David J. Milan, Filip K. Swirski, Kevin R. King, Aaron D. Aguirre, Patrick T. Ellinor, and Matthias Nahrendorf
…Oxidative stress was imaged after intravenous injection of CellROX Deep Red Reagent (C10422, Thermo Fisher Scientific, 20 μl diluted in 100 μl of PBS). Hearts were sliced in 1-mm sections for immediate imaging using a Sapphire Biomolecular Imager (Azure Biosystems)…
Zhiwu Tan, Mei Sum Chiu, Chi Wing Yan, Kwan Man, Zhiwei Chen
…resected REN tumor fluorescence was imaged with a Sapphire Biomolecular Imager (Azure Biosystems) after surgical resection…
Jung Kim, Sung Kyun Lee, Jong-Hwan Lee, Hye-Yeon Kim, Nam Hoon Kim, Chang Hoon Lee, Chang-Seop Lee, and Hong Gi Kim
…NP antigen in coating buffer A (CB07100, Invitrogen, USA) was prepared at 1, 3, or 5 μg mL−1 and added to the ZnO-NW MP. Next, the anti-NP polyclonal antibody (32, 160, or 800 ng mL−1) in assay buffer (DS98200, Invitrogen, USA) was applied to the plate for 1 h, followed by the Alexa Fluor 488-conjugated secondary antibody (A-11034; 1 mg mL−1) for a further hour. The plate was washed between these steps. The fluorescence signal was measured in a microplate reader and a image taken under a laser scanning imager (Sapphire Biomolecular Imager, Azure biosystems, USA)…
Tara N. Guhr Lee, Deborah M. Cholon, Nancy L. Quinney, Martina Gentzsch, Charles R. Esther Jr
…Blots were probed with mouse monoclonal anti-CFTR antibodies and then with IRDye 680– goat anti-mouse immunoglobulin G (Molecular Probes). Anti-actin (Cell Signaling) was used as a loading control. Protein bands were visualized using a Sapphire Biomolecular Imager (Azure Biosystems)…
Lin Shang, Dongmei Deng, Sanne Roffel, and Susan Gibbs
tern Blots Fig. 6. Toll-like receptor (TLR) protein expression in reconstructed human skin (RHS) and gingiva (RHG). TLR1 and 4 proteins are shown together with reference tubulin expression. TLR2, 3, 5, and 6 were under the detectable level (data now shown). Data are representative of three independent experiments ee times in PBST d dye-conjugated use (1:7500 for :7500 for TLR1, 2, blots were ecular Imager rnia).…” WESTERN BLOTS Differential influence of Streptococcus …membranes were washed three times in PBST and further incubated with infrared dyeconjugated secondary antibodies against mouse (1:7500 for TLR3, 4, or 5) or against rabbit (1:7500 for TLR1, 2, 6, or tubulin). After washing, the blots were visualized using Sapphire Biomolecular Imager (Azure biosystems, Dublin California)…
Gaurav Shrivastava, Paola Carolina Valenzuela-Leon, Andrezza Campos Chagas, Olivia Kern, Karina Botello, Yixiang Zhang, Ines Martin-Martin, Markus Berger Oliveira, Lucas Tirloni, and Eric Calvo
…Finally, the plates were scanned at 700 and 800 nm, and the intensity of the labeled proteins was measured using the Azure sapphire biomolecular imager (Azure Biosystems)
Kensei Kishimoto, Catera L. Wilder, Justin Buchanan, Minh Nguyen, Chidera Okeke, Alexander Hoffmann and Quen J. Cheng
…Nuclear extracts…were incubated with P32-labaled oligonucleotide probes…The reaction mixtures were run on a 5% acrylamide (30:0.8) gel with 5% glycerol and TGE buffer (24.8 mM Tris, 190 mM glycine, 1 mM EDTA) at 200V for 1 hour and 45 mins. The gels were dried and imaged on a Sapphire Biomolecular imager in phosphor mode (Azure Biosystems, Dublin, CA).
Joseph Russo, Cary T. Mundell, Phillida A. Charley, Carol Wilusz, and Jeffrey Wilusz
…The samples were then resolved via denaturing PAGE, dried, exposed to a phosphor screen, and viewed via an Azure Sapphire Biomolecular Imager providing sufficient sensitivity to observe all required bands with one exposure.
Xupeng Hong, Yuka Imamura Kawasawa, Stephan Menne, Jianming Hu
…Viral DNAs were resolved on 1.2% agarose gel and detected by 32P-labeled HBV or WHV DNA probes…DNA signals from Southern blot analysis were detected by Sapphire Biomolecular Imager (Azure Biosystems) and quantified using the Image Lab system 6.0.1 (Bio-Rad).
Carla Bianca Luena Victorio, Joanne Ong, Jing Yang Tham, Marie Jennifer Reolo, Wisna Novera, Rasha Msallam, Satoru Watanabe, Shirin Kalimuddin, Jenny G. Low, Subhash G. Vasudevan, Ann‐Marie Chacko
…Infected mice were injected i.v. with 10 MBq [18 F]FDG and tissues were harvested following a 60-min tracer uptake. Freshly isolated wholemount lymphoid tissues were immediately exposed to multi-purpose phosphoscreen (BAS-IP MS) for 30 min. Fresh tissues with high tracer uptake were exposed to super-resolution (BAS-IP SR) phosphoscreen for 5 min (GE Healthcare Life Sciences, USA). [ 8 F]FDG standards at 2/3 serial dilution from 600 to 0 kBq were mounted together with mouse tissue for calibration of digital autoradiography (DAR) images. Screens were then scanned using the Sapphire Biomolecular Imager (Azure Biosystems, USA) at 100-μm resolution…
Petronella R. Hove, Forgivemore Magunda, Maria Angela de Mello Marques, M. Nurul Islam, Marisa R. Harton, Mary Jackson, John T. Belisle
…The lipid extracts were resolved by thin-layer chromatography (TLC) using silica gel G60 TLC plates and chloroform/methanol (90:10 v/v) as the mobile phase. Reference non-radioactive standards were resolved concurrently and developed separately from the blot containing radioactive lipids with phosphomolybdic acid stain. Following this, blot with reference standard was matched with TLC blot to mark resolution of lipids. After development radiolabeled lipids were imaged using Azure Sapphire Biomolecular Imager (Azure Biosystems Inc, Dublin, CA)…
Jong-Hwan Lee, Minsuk Choi, Yujin Jung, Sung Kyun Lee, Chang-Seop Lee, Jung Kim, Jongwoo Kim, Nam Hoon Kim, Bum-Tae Kim, Hong Gi Kim
…the intensity of the test and control lines were converted to peak histograms using a Sapphire Biomolecular Imager.
Jong-Hwan Lee, Minsuk Choi, Yujin Jung, Sung Kyun Lee, Chang-Seop Lee, Jung Kim, Jongwoo Kim, Nam Hoon Kim, Bum-Tae Kim, Hong Gi Kim
…The clonogenic recovery potential gives insight into the capability of the cells to form a new tumor (metastasis). Therefore, 500 cells/well were seeded on a 6-well-plate and treated for 10 days with ART. Untreated cells served as controls. RCC cells were subsequently fixed with 85% MeOH/15% AcOH and stained with Coomassie (0.5 g Coomassie Blue G250 (SigmaAldrich, Darmstadt, Germany), 75 mL AcOH, 200 mL MeOH, 725 mL distilled water). Amount and size of cell clone colonies were measured with a biomolecular imager (Sapphire, Azure Biosystems, Biozym, Hess. Oldendorf, Germany)…
|Parameter||Azure Sapphire FL|
|Detection modes||Fluorescence, phosphor imaging, densitometry, and chemiluminescence*|
|Detectors||Confocal scanning with lasers and PMT or APD detectors|
|Pixel size||5, 10, 20, 25, 50, 75, 100, 150, 200, 500, 1000 μm|
|Supported wavelength range||375 – 900 nm|
|Excitation range||375 – 850 nm|
|Emission range||380 – 900 nm|
|Maximum field of view (FOV):||25 cm x 25 cm|
|Z-plane focal range||7 mm; minimum delta 0.01mm, from -1 mm below the glass to 6 mm above the glass|
|Standard optical modules||Solid-state laser diodes emitting at 375, 488, 532, 638, 658, 685, and 784 nm. Phosphor Standard Optical Module available.|
|Custom Optical Modules||Custom lasers and filters are available upon request to provide exceptional research flexibility.|
|Wide dynamic range||24-bit|
|Clearance from platen to lid||4 cm|
|Animal imaging||Yes, with 5 built-in anesthesia ports compatible with standard small animal anesthesia tubing|
|Unit dimensions (W x H x D)||63.0 cm x 39.9 cm x 59.3 cm / 24.8” x 15.7” x 23.3"|
|Weight||43.5 kg / 95.9 lbs|
|Power requirements||100 – 240 VAC ± 10%, 50/60 Hz|
|Connection to computer||Fast LAN (Ethernet) cable|
|Certifications||CE, cTUVus, CB scheme|
*Chemiluminescence module sold separately
Azure Imagers have been published in over 2,000 publications worldwide. Our high-resolution CCD cameras are the reason behind reproducible data and publication-ready images.
Contains 7 Foot Cat6 (UTP) Ethernet Cable and 4 port Netgear ethernet switch.
Fixture designed to hold up to four standard 96-well plates with optically clear bottoms
Designed to hold up to 15 standard microscope slides
18 inches of tubing with luer fittings
Set of five 7mm anesthesia nose cones for a tight fit with laboratory mice
|Brochure||Azure Sapphire FL Biomolecular Imager Brochure||DOWNLOAD|
|Booklet||Sapphire FL Biomolecular Imager Applications and Publications||DOWNLOAD|
|User Manual||Sapphire FL User Manual||DOWNLOAD|
|App Note||Fluorescent IHC Imaging of Tumor Tissue Arrays on the Sapphire FL||DOWNLOAD|
|App Note||In vivo imaging and monitoring of RFP+ tumors in mice using the Sapphire FL||DOWNLOAD|
|App Note||Multicolor Fluorescent Imaging of Cultured Cells on the Sapphire FL: Live/Dead (AO/PI) Assays||DOWNLOAD|
|App Note||Imaging YFP-expressing cells with the Sapphire FL||DOWNLOAD|
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