Sapphire FL Biomolecular Imager

Name: Sapphire FL
Brand: Azure Biosystems
SKU: IS4000
Availability: InStock
Rating: 5 (3 reviews)

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Unmatched Application Versatility and Performance

24-bit Dynamic Range

Extended dynamic range allows imaging of both bright and weak bands without experiencing saturation.

5 Micron Resolution

From Western blots and gels, to microscope slides and live animal imaging, adjustable 5-1000 µm resolution ensures unparalleled flexibility.

Custom Design Lasers and Filters

No limits – easily swap lasers and filters to achieve the perfect system to image your sample

Adjustable focus for sample thickness

Whether imaging Western blots, gels, 96-well plates, or tissue slides, the adjustable Z-plane ensures optimal focus for your sample.

Key Features

Flexibility with uncompromising performance

The Sapphire FL is the second generation Sapphire and ultimate biomolecular imager for FLEXIBILITY. With customizable and user-changeable laser and filter modules, it easily adapts to a lab’s changing needs and advancing research. The Sapphire FL offers customizable and user-changeable optical modules, 5–1000 μm resolution scans, a Z-plane range from -1.0 to +6 mm, five anesthesia ports for imaging living animals, chemiluminescence detection through a Chemiluminescence Module, and much more.

Unlimited Laser and Filter Wavelength combinations

Removing optical module from Sapphire FL — 1. The Sapphire FL gives you the freedom to choose the best laser combination for your studies. Begin by removing the optical module.

How to load new laser and filter combination into the Sapphire FL. — 3. Load new laser and filter combination into the Sapphire FL.

Sapphire FL Standard Optical Module List — More optical module options available upon request.

It features a unique, patent-pending design of interchangeable and customizable laser and filter modules, enabling a virtually infinite number of spectral combinations. A broad range of excitation and emission wavelengths, as well as phosphor imaging, are supported.

The Sapphire FL is the only biomolecular scanner in its class that reaches into the UV spectrum, allowing multiplexing of UV dyes with dyes in the NIR. Using the 375 Standard Optical Module, it is able to image common DNA stains, such as Dapi and Hoechst.

Sapphire FL Applications — The Sapphire FL boasts the widest wavelength range of any biomolecular imaging system with the support of wavelengths between 375 – 900 nm, allowing it to support imaging of Western blots, gels, phosphor screens, tissues, slides, arrays and much more.

In-cell western assay — **In-cell Western assay performed using AzureCyto In-cell Western Kit.** A 96-well plate was imaged on the Azure Sapphire FL using the 532, 685, and 784 Standard Optical Modules at a resolution of 10µm with the focus set to +3.00mm.

AO/PI staining on PBMCs with red and green cells — PBMCs subjected to AO/PI staining were imaged with the Sapphire FL Biomolecular Imager at 10 μm. Living AO-stained cells appear green while dead or dying PI-stained cells appear red.

Mouse brain section, stained for two protein markers, imaged on the Sapphire FL — Mouse brain section, stained for two protein markers and DAPI. Imaged on the Sapphire FL at 5 µm, with a z-plane of 0.21mm.

Backside of sheep tongue scanned d at 10 micron with Azure Sapphire Biomolecular Imager — Backside of sheep tongue. Imaged on the Sapphire FL with 658nm and 488nm laser modules at 10 µm.

Western blot detected with NIR immunodetection, using TotalStainQ

4-color multiplex Western blot from Azure Sapphire — Digital image of 4-color multiplex Western blot. Using distinct fluorescent and near-infrared targeting antibodies can detect each wavelength and merge them into a four-color multiplex image. No background noise or bleeding between channels. Image captured with the Sapphire Biomolecular Imager

Mini Leaf Fern Prothallia & Sporangia scanned at 10 micron using Azure Sapphire — Mini Leaf Fern Prothallia & Sporangia. Imaged on the Sapphire at 10 μm.

Application Flexibility

The flexibility of the Sapphire FL guarantees you the freedom to confidently choose the best dyes and chemistry for any experiment. Custom optical modules can excite any dye at its peak, resulting in the highest possible signal for any fluorophore.

In addition to fluorescence, the Sapphire FL also excels at imaging phosphor screens and chemiluminescence using the optional Chemiluminescence Module.

Sensitive Fluorescent Detection

High sensitivity allows femtogram detection of proteins labeled with common fluorescent dyes.

No other system on the market matches the Sapphire FL’s coverage across the fluorescent spectrum. Extend your reach into the UV spectrum with the 375 Standard Optical Module.

Total Flexibility with Phosphor Imaging

The Sapphire FL is a compact phosphor imager that incorporates exceptional visible fluorescent and near infra-red (NIR) scanning, along with true chemiluminescent and white light imaging, to make it a true lab workhorse.

It is one of only two scanning systems in its class that offers phosphor imaging, a high sensitivity assay that requires radioactivity. The Sapphire FL is able to scan storage phosphor screens with exceptional dynamic range and image quality, thanks to the use of laser excitation and photon multiplier tube (PMT) detection.

In Vivo Imaging

Sapphire FL anesthesia ports — The Sapphire FL has five anesthesia ports, which allow for fluorescent and phosphor imaging of live small animals, such as mice.

Experimental mice were injected with 4T1 tumor cells expressing RFP, while controls were subjected to RFP-negative 4T1 cells. The mice were imaged at multiple timepoints to monitor tumor growth and progression over time. Tumors were measured at Day 4 and Day 11 post-injection and showed substantial growth over this period. Fluorescent signal from the RFP+ tumors also increased over this time and was captured in vivo using the Sapphire FL — Fluorescently labeled (RFP+) tumors are visible in in vivo images of whole mice captured on the Sapphire FL at 100 µm resolution using the 532 Standard Optical Module with intensity setting 6. The focus height was set to 0.00 mm.

Six mice under anesthesia before imaging on Sapphire FL — The Sapphire FL allows for live imaging of small animals, such as the mice shown here. In vivo assays are an important step in health-related research, for cell migration studies and pre-clinical drug testing. The imaging of live animals is possible through the Sapphire FL's five anesthesia ports and 4 cm of clearance from lid to platen.

The Sapphire FL imager makes imaging of live animals possible. Living mice or other small animals can be fluorescently imaged under anesthesia using any of the five built-in anesthesia ports.

The wide imaging platform can accommodate specimens with depth (up to 4 cm), allowing small animals, plants, petri dishes, and other large biological samples to be imaged.

Distinguish between Subtle Differences in Expression with Extended Dynamic Range (EDR)

Extended dynamic range allows you to image both bright and weak bands without experiencing saturation. This is ideal for the samples that feature strong and weak expressing proteins.

EDR extends dynamic range to 24 bits of data.

Adjustable Focus

Some samples can be imaged on the glass (Western blots), while others are raised above the glass surface (96-well plates). The Sapphire FL can image them all.

Some samples have multiple focal points within them, such as tissue slides and organs. The Sapphire FL can image those too.

It offers a 7mm software-controlled adjustable focal plane, so you can scan your sample at multiple depths to ensure you see all your data has to offer.

5-micron Resolution

The Sapphire FL scans between 5 – 1000 µm resolution to ensure clear visualization of detailed samples. Image complex and minute samples, including tissue and microarray slides, with accuracy. Triage multiple slides at high resolution before microscopic analysis.

Chemiluminescent Imaging When You Need It

An optional Chemiluminescence Module adds high-resolution, quantitative chemiluminescence and visible imaging.

It allows you to express proteins with femtogram sensitivity and gives you the ability to capture color marker images.

What is the Sapphire FL biomolecular imager used for?

The Sapphire FL biomolecular imager is used for detecting and quantifying proteins and nucleic acids across a wide range of imaging applications, including western blots, tissue and microarray slides, multi-well plates, gel documentation, in vivo imaging, and phosphor imaging. It supports both chemiluminescent and fluorescent detection, enabling researchers to analyze multiple biomolecules with high precision.

What makes the Sapphire FL different from other western blot imaging systems?

The Sapphire FL stands out due to its modular design, allowing optimal excitation and detection of fluorophores from the UV to the NIR. Labs are free to choose the wavelengths that best support their research, rather than being locked-in to preselected channels. In addition, the Sapphire FL offers laser-based excitation, which provides higher sensitivity, lower background noise, and more accurate quantitation compared to LED-based systems. It also enables true multiplex imaging, allowing multiple targets to be detected simultaneously on the same blot without stripping and reprobing.

Can the Sapphire FL perform multiplex fluorescence imaging?

Yes, the Sapphire FL is designed for multiplex fluorescence imaging and can detect multiple targets in a single experiment. With a wide selection of standard laser modules, researchers can optimally detect several proteins or nucleic acid targets simultaneously, improving efficiency and conserving samples.

How does the Sapphire FL improve data accuracy and reproducibility?

The Sapphire FL improves accuracy through its high-resolution imaging, wide dynamic range, and consistent laser-based excitation. These features reduce variability between experiments and allow for precise quantitation of signal intensity, helping ensure reproducible and reliable results.

What types of applications is the Sapphire FL best suited for?

The Sapphire FL is well suited for phosphor and fluorescence imaging applications such as western blot imaging, in-cell western imaging, protein quantification, nucleic acid detection, gel imaging, tissue and microarray slide imaging, and phosphor screen imaging. Its versatility makes it useful for both basic research and advanced applications requiring high sensitivity and multiplex capability.

Frequently Asked Questions

Trusted by Scientists Worldwide

Applications

The new Sapphire FL provides you with the versatility to do more applications with one system. Browse the list of published examples below to see what the SFL is capable of.

Fluorescence Imaging

Tissue Imaging
In-Gel Imaging
Lateral Flow Assay Development
2D Dige

White Light and Autofluorescence

Rescue of autoimmune hepatitis by soluble MHC class II molecules in an altered concanavalin A-induced experimental model

Katerina Bakela, Maria Georgia Dimitraki, Evangelia Skoufa, Irene Athanassakis

…Liver tissues were isolated 6 months after the ConA and the ConA + sMHCII treatment of mice and fixed using PFA 4%, under rotation, at 4°C for 24 hours. Azure Biosystems Sapphire™ Biomolecular Imager (Azure Biosystems, Dublin, CA 94568 USA) was used in order to scan the liver tissues in 10-μm resolution. This instrument combines NIR fluorescence (both long and short), RGB fluorescence, chemiluminescent, and phosphor imaging capabilities, while using four solid state lasers as excitation sources (450, 520, 660, and 780 nm). The application of the four-channel fluorescence mode at 10-μm resolution, could detect gross anatomy and morphology of liver tissues, mainly based on tissue autofluorescence…

Animal Model Exp Med. 2020;3(3):264–272

Immunohistochemistry

The ISL LIM-homeobox 2 transcription factor is negatively regulated by circadian adrenergic signaling to repress the expression of Aanat in pinealocytes of the rat pineal gland

Mattern KMJ, Blancas–Velázquez AS, Ngo MT, et al.

Cryostat sections of 12-μm thickness were mounted on slides and blocked in 5% normal donkey serum (Abcam). Immunohistochemistry was performed as previously described…Sections were incubated in primary antibody at 4°C overnight and in secondary antibody at room temperature for…1 h if used for whole section imaging…Images of whole sections were taken on an Azure Sapphire Biomolecular Imager (Azure Biosystems). Fluorescence signal intensity after background substraction [sic] was quantified with the Azure Spot software… “Western blot” for image acquisition, an Azure Sapphire Biomolecular Imager was used. The density of bands was quantified with the Azure Spot software.

J Pineal Res. 2023;e12905

Figure 3. Diurnal dynamics of Isl2 expression in the pineal gland of the adult rat…(C) Two representative western blot lanes of samples containing protein extracted from pineal glands were harvested at ZT10 and ZT22 to visualize the difference of ISL2 abundancy in daytime versus nighttime samples. Rat ISL2 protein (NP_065204) has a predicted molecular weight of 39.7 kDa. Housekeeping proteins ACTB and GAPDH were used as loading controls…(D) Two representative immunohistochemical stainings in brain sections from brains removed at ZT10 and ZT22 to visualize differences in ISL2 abundancy. Scale bars = 1 mm. (E) Quantification of ISL2 levels in the individual lanes of the western blot shown in (C).

laser scanning, ISL2 was detected using a sheep anti-hlslet-2 primary antibody and an Alexa Fluor™ 790 donkey anti-sheep secondary antibody. For Western blotting, ISL2 was detected with a sheep-anti-human Islet-2 antibody and a donkey-anti-sheep Alexa Fluor™ 680 secondary antibody. ACTB was detected with a rabbit-anti-beta actin antibody and a donkey-anti-rabbit Alexa Fluor™ 790 secondary antibody.

Parallel imaging of coagulation pathway proteases activated protein C, thrombin, and factor Xa in human plasma

Sylwia Modrzycka, Sonia Kołt, Stéphanie G. I. Polderdijk, Ty E. Adams, Stanisław Potoczek, James A. Huntington, Paulina Kasperkiewicz and Marcin Drąg

…For the simultaneous coagulation factor labeling, human plasma was incubated with 5 μM of each fluorescently labeled probe…and then 5 μL of each sample was run onto a 10% MES (w/v) 15-well gel…The gel was then directly scanned at 520 nm for Cy3, 658 nm for Cy5, and 784 nm for Cy7 detection using an Azure Biosystems Sapphire Biomolecular Imager and Azure Spot Analysis Software.

Chem Sci. 2022;13(23):6813-6029

Figure 6. Coagulation factor (APC, thrombin, fXa) labeling in human plasma. (A) Probe concentration optimization assay. Human plasma was
incubated with each fluorescent ABP separately at various probe concentrations ranging from 1 to 20 μM for 60 min at 37 °C. The samples
were then subjected to SDS–PAGE analysis, transferred to a membrane, immunostained with the appropriate antibody, and imaged using an
Azure Biosystems Sapphire Biomolecular Imager as follows: for APC at 658 nm (for Cy5 detection) and 488 nm (for antibody detection), for
thrombin at 784 nm (for Cy7 detection) and 658 nm (for antibody detection), for fXa at 520 nm (for Cy3 detection) and 658 nm (for antibody
detection). The results are representative of at least 3 replicates. (B) Graphical scheme of the methodology used for simultaneous coagulation
factor detection in human plasma. (C) Simultaneous coagulation factor labeling in human plasma. Human plasma was incubated with 5 μM of
each fluorescently labeled ABP and subjected to SDS–PAGE analysis. Direct in-gel analysis was performed with lasers of 520 nm for Cy3, 658
nm for Cy5, and 784 nm for Cy7 using an Azure Biosystems Sapphire Biomolecular Imager. The results are representative of at least 3 replicates. — Figure 6. Coagulation factor (APC, thrombin, fXa) labeling in human plasma. (A) Probe concentration optimization assay. Human plasma was incubated with each fluorescent ABP separately at various probe concentrations ranging from 1 to 20 μM for 60 min at 37 °C. The samples were then subjected to SDS–PAGE analysis, transferred to a membrane, immunostained with the appropriate antibody, and imaged using an Azure Biosystems Sapphire Biomolecular Imager as follows: for APC at 658 nm (for Cy5 detection) and 488 nm (for antibody detection), for thrombin at 784 nm (for Cy7 detection) and 658 nm (for antibody detection), for fXa at 520 nm (for Cy3 detection) and 658 nm (for antibody detection). The results are representative of at least 3 replicates. (B) Graphical scheme of the methodology used for simultaneous coagulation factor detection in human plasma. (C) Simultaneous coagulation factor labeling in human plasma. Human plasma was incubated with 5 μM of each fluorescently labeled ABP and subjected to SDS–PAGE analysis. Direct in-gel analysis was performed with lasers of 520 nm for Cy3, 658 nm for Cy5, and 784 nm for Cy7 using an Azure Biosystems Sapphire Biomolecular Imager. The results are representative of at least 3 replicates.

Method for the elucidation of LAMP products captured on lateral flow strips in a point of care test for HPV 16

Lena Landaverde, Winnie Wong, Gabriela Hernandez, Andy Fan & Catherine Klapperich

…To identify 5′-FAM-specific LAMP products, unstained agarose and acrylamide gels were imaged under 488-nm laser excitation using the Sapphire Biomolecular Imager…

Anal Bioanal Chem. 2020;412(24):6199-8209

Figure 4. FAM band comparisons of pvuII-digested and undigested lateral flow strip elutions. Three different representations of the LAMP
amplicon products are shown. Gel a and b are LAMP amplicons digested with pvuII (independent of LFS). Gels c, d, f, and g are eluted products
from the LFS itself. Furthermore, gels c and d contain uncleaved LFS-LAMP products (pvuII−), whereas gels f and g are cleaved LFS-LAMP
products (pvuII+). The FAM bands 94–114 (band C) bp and 71–80 bp (b and D) are highlighted as the HPV 16 FAM amplicons. Gel b also shows
the previously sequenced bands of 146 (band A) and 92 (band B). The LFS strips that DNA was eluted from are shown in LFS images e and h
of Fig. 4. The gels in panels a and b are pvuII-digested LAMP products; the lateral flow strips results are equivalent to the LFS shown in panel h. — Figure 4. FAM band comparisons of pvuII-digested and undigested lateral flow strip elutions. Three different representations of the LAMP amplicon products are shown. Gel a and b are LAMP amplicons digested with pvuII (independent of LFS). Gels c, d, f, and g are eluted products from the LFS itself. Furthermore, gels c and d contain uncleaved LFS-LAMP products (pvuII−), whereas gels f and g are cleaved LFS-LAMP products (pvuII+). The FAM bands 94–114 (band C) bp and 71–80 bp (b and D) are highlighted as the HPV 16 FAM amplicons. Gel b also shows the previously sequenced bands of 146 (band A) and 92 (band B). The LFS strips that DNA was eluted from are shown in LFS images e and h of Fig. 4. The gels in panels a and b are pvuII-digested LAMP products; the lateral flow strips results are equivalent to the LFS shown in panel h.

Proteomic Analysis of Endometrial Cancer Tissues from Patients with Type 2 Diabetes Mellitus

Muhammad Mujammami, Mohamed Rafiullah, Assim A. Alfadda, Khalid Akkour, Ibrahim O. Alanazi, Afshan Masood, Mohthash Musambil, Hani Alhalal, Maria Arafah, Anas M. Abdel Rahman and Hicham Benabdelkamel

…The gels were scanned with Sapphire Biomolecular Imager (Azure Biosystems…) and digitalized via the image analysis software Sapphire Capture system (Azure Biosystems…

Life.12(4):491

Gel Shift Assay
Organ Imaging
Tumor Imaging
Modified Microplates

(example of DNA-Protein Binding Assay)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing

Brian J. Caldwell, Andrew S. Norris, Caroline F. Karbowski, Alyssa M. Wiegand, Vicki H. Wysocki & Charles E. Bell

…A gel shift DNA binding assay used two complementary 50-mer oligonucleotides labeled at the 5′-end with either Cy3 or Cy5…For visualization 17.5 μl of each complex was mixed with 7.5 μl Orange G dye…Gels were imaged using a Sapphire Biomolecular Imager (Azure Biosystems) with Sapphire Capture Software (version 1.12.0921.0). Scanning parameters for Fig. 8 were pixel size 100 μm, scan speed high, 2.38mm focus, intensity 2 for Cy5, intensity 4 for Cy3, black lighting 50, white 37186, gamma 1.37. Scanning parameters for Supplementary Fig. 1a, b were intensity 1 for Cy5, intensity 2 for Cy3, black lighting 50, white 15362, gamma 0.88.

Nat Commun. 2022;13(1):7855

Figure 8 (a). Mutational analysis. Each panel shows a gel-shift assay with 3.6 μM of LiRecT mixed with different combinations of Cy3- and Cy5-
labeled 50mer oligonucleotides (25 μM nucleotides). Lanes C3, C5: each oligo without protein. Lanes 3, 5: LiRecT mixed with each individual
oligonucleotide (Cy3-50mer or Cy5-50mer) to form a ssDNA complex. Lanes 35: LiRecT incubated with Cy3- 50mer at 37° for 15 min, followed
by addition of Cy5-50mer and incubation for an additional 15 min to form the duplex intermediate (yellow band). — Figure 8 (a). Mutational analysis. Each panel shows a gel-shift assay with 3.6 μM of LiRecT mixed with different combinations of Cy3- and Cy5- labeled 50mer oligonucleotides (25 μM nucleotides). Lanes C3, C5: each oligo without protein. Lanes 3, 5: LiRecT mixed with each individual oligonucleotide (Cy3-50mer or Cy5-50mer) to form a ssDNA complex. Lanes 35: LiRecT incubated with Cy3- 50mer at 37° for 15 min, followed by addition of Cy5-50mer and incubation for an additional 15 min to form the duplex intermediate (yellow band).

Visible Fluorescence

Neutrophils incite and macrophages avert electrical storm after myocardial infarction

Jana Grune, Andrew J. M. Lewis, Masahiro Yamazoe, Maarten Hulsmans, David Rohde, Ling Xiao, Shuang Zhang, Christiane Ott, David M. Calcagno, Yirong Zhou, Kerstin Timm, Mayooran Shanmuganathan, Fadi E. Pulous, Maximilian J. Schloss, Brody H. Foy, Diane Capen, Claudio Vinegoni, Gregory R. Wojtkiewicz, Yoshiko Iwamoto, Tilman Grune, Dennis Brown, John Higgins, Vanessa M. Ferreira, Neil Herring, Keith M. Channon, Stefan Neubauer, Oxford Acute Myocardial Infarction (OxAMI) Study,* David E. Sosnovik, David J. Milan, Filip K. Swirski, Kevin R. King, Aaron D. Aguirre, Patrick T. Ellinor, and Matthias Nahrendorf

…Oxidative stress was imaged after intravenous injection of CellROX Deep Red Reagent (C10422, Thermo Fisher Scientific, 20 μl diluted in 100 μl of PBS). Hearts were sliced in 1-mm sections for immediate imaging using a Sapphire Biomolecular Imager (Azure Biosystems)…

Nat Cardiovasc Res. 2022;1(7):649–664

Visible Fluorescence

Eliminating mesothelioma by AAV-vectored, PD1-based vaccination in the tumor microenvironment

Zhiwu Tan, Mei Sum Chiu, Chi Wing Yan, Kwan Man, Zhiwei Chen

…resected REN tumor fluorescence was imaged with a Sapphire Biomolecular Imager (Azure Biosystems) after surgical resection…

Mol Ther Oncolytics. 2021;29:373-386

ZnO Nanowire-Based Early Detection of SARS-CoV-2 Antibody Responses in Asymptomatic Patients with COVID-19

Jung Kim, Sung Kyun Lee, Jong-Hwan Lee, Hye-Yeon Kim, Nam Hoon Kim, Chang Hoon Lee, Chang-Seop Lee, and Hong Gi Kim

…NP antigen in coating buffer A (CB07100, Invitrogen, USA) was prepared at 1, 3, or 5 μg mL−1 and added to the ZnO-NW MP. Next, the anti-NP polyclonal antibody (32, 160, or 800 ng mL−1) in assay buffer (DS98200, Invitrogen, USA) was applied to the plate for 1 h, followed by the Alexa Fluor 488-conjugated secondary antibody (A-11034; 1 mg mL−1) for a further hour. The plate was washed between these steps. The fluorescence signal was measured in a microplate reader and a image taken under a laser scanning imager (Sapphire Biomolecular Imager, Azure biosystems, USA)…

Adv Mater Interfaces. 2022;9(14):2102046

Chemiluminescence Imaging

Western Blots
Protein Arrays
Plant Leaf Bioluminescence

Shikonin Reduces Growth of Docetaxel-Resistant Prostate Cancer Cells Mainly through Necroptosis

Sascha D. Markowitsch, Kira M. Juetter, Patricia Schupp, Kristine Hauschulte, Olesya Vakhrusheva, Kimberly Sue Slade, Anita Thomas, Igor Tsaur, Jindrich Cinatl, Jr., Martin Michaelis, Thomas Efferth, Axel Haferkamp and Eva Juengel

…To explore the expression and activity of cell cycle and cell death regulating proteins, Western blot analysis was performed…The membranes were incubated with ECL detection reagent (AC2204, Azure Biosystems, Munich, Germany) to visualize proteins with a Sapphire Imager (Azure Biosystems, Munich, Germany)…Cell death regulating proteins were normalized to total protein that was quantified by staining total protein from all membranes with Coomassie brilliant blue and measuring with a Sapphire Imager…

Cancers. 2021;13(4):882

NonO Is a Novel Co-factor of PRDM1 and Regulates Inflammatory Response in Monocyte Derived-Dendritic Cells

Kyungwoo Lee, Su Hwa Jang, Hong Tian and Sun Jung Kim

…Proteins bound by antibody were visualized by ECL (Thermo Scientific, #34580 or Advansta, K-12045) and sapphire biomolecular imager (Azure Biosystems)…

Front Immunol. 2020;11:1436

Effects of prolactin on ventricular myocyte shortening and calcium transport in the streptozotocin-induced diabetic rat

Frank C. Howarth, Gunnar Norstedt, Oleksiy I. Boldyriev, Muhammad A. Qureshi, Ozaz Mohamed, Khatija Parekh, Balaji Venkataraman, Sandeep Subramanya, Anatoliy Shmygol, Lina T. Al Kury

…The blots were developed using the Super Signal West Pico Plus chemiluminescent substrate (34577, Thermo Scientific, Rockford, IL, USA). The blot images were acquired using a Sapphire Biomolecular Imager (Azure Biosystems, Dublin, California, USA) using chemiluminescent detection of HRP, coupled with color image acquisition of the protein ladder…

Heliyon. 2020;6(4):e03797

A dominant negative mitofusin causes mitochondrial perinuclear clusters because of aberrant tethering

Stephanie R Sloat, Suzanne Hoppins

…Membranes were developed in Radiance Plus Chemiluminescent HRP Substrate (Azure Biosystems) for 5 min and imaged on a Sapphire Biomolecular Imager (Azure Biosystems). Band intensities were quantified using AzureSpot analysis software (Azure Biosystems)… Western blot images for shRNA were acquired on Sapphire, and quantification was performed with AzureSpot. Knockdown quantification was normalized using whole-protein stain (Azure Biosystems)

Life Sci Alliance. 2022;6(1):e202101305

Figure 7. Mfn1S329P is defective for GTP-dependent oligomerization. (A) Representative blue native–PAGE of mitochondria isolated from Flp-In TREx cells expressing Mfn1WT-FLAG or Mfn1S329P-FLAG after incubation with 0.2 μg/ml TET for 4 h. Mitochondria were untreated or incubated in the presence of GMP-PNP (PNP), followed by solubilization and separation by blue native–PAGE and immunoblotted with α-FLAG. Arrow indicates ~200-kD species, closed arrowhead indicates ~320-kD species, and open arrowhead indicates ~450-kD species. Molecular-weight markers are indicated in kD on left. (A, B) Quantification of native mitofusin species indicated in A. Error bars represent mean ± SEM from n = 3 separate experiments.

Engineered unnatural ubiquitin for optimal detection of deubiquitinating enzymes

Wioletta Rut, Mikolaj Zmudzinski, Scott J. Snipas, Miklos Bekes, Tony T. Huang and Marcin Drag

…Biotinylated Ub-based probes were detected with a fluorescent streptavidin Alexa Fluor 647 conjugate (1 : 10 000) in TBS-T with 1% BSA, and UCH-L3 was detected with a mouse antihuman monoclonal IgG1 antibody (1 : 1000) and fluorescent goat anti-mouse (1 : 10 000) using an Azure Biosystems Sapphire Biomolecular Imager and Azure Spot Analysis Software.

Chem Sci. 2020;11(23):6058-6069

In vitro Evaluation of ASCs and HUVECs Co-cultures in 3D Biodegradable Hydrogels on Neurite Outgrowth and Vascular Organization

Luís A. Rocha, Eduardo D. Gomes, João L. Afonso, Sara Granja, Fatima Baltazar, Nuno A. Silva, Molly S. Shoichet, Rui A. Sousa, David A. Learmonth and Antonio J. Salgado

…The evaluation of the angiogenic and neurotrophic profile of the previously obtained secretomes was performed using the Human Neuro Discovery Array C1 and Human Angiogenesis Array C1 (RayBiotech, United States)…Finally, the chemiluminescence image of each membrane was obtained using a Sapphire Biomolecular Imager (Azure Biosystems, United States). The intensity of each dot was quantified using the AzureSpot software (Azure Biosystems, United States)…

Front Cell Dev Biol. 2020;8:489

A species-specific functional module controls formation of pollen apertures

Byung Ha Lee, Rui Wang, Ingrid M. Moberg, Sarah H. Reeder, Prativa Amom, Michelle H. Tan, Katelyn Amstutz, Pallavi Chandna, Adam Helton, Ekaterina P. Andrianova, Igor B. Zhulin, and Anna A. Dobritsa

Agrobacteria were collected and resuspended in infiltration buffer (10 mM MgCl2, 10 mM MES, 150 μM acetosyringone) at a final concentration of OD600 = 0.8. Pairwise combinations of suspensions were infiltrated into young tobacco leaves, which were then allowed to grow for 3 d in light. A total 12–16 leaves were collected from five to ten plants, the abaxial side of leaves was sprayed with 1 mM luciferin (Biosynth, L-8220) and kept in the dark at 4 °C for 30 min. The bioluminescence images were captured using Azure Sapphire Biomolecule Imager (Azure Biosystems) and converted to heatmaps using the 16-colour look-up table from ImageJ v.1.53a…

Nat Plants. 2021;7(7):966-978

Figure 6c. Split-luciferase assay testing the ability of SlINP1 and SlINP2 to interact. Tobacco leaves were divided into sectors co-expressing
indicated proteins containing the N-terminal (NLuc) and C-terminal (CLuc) parts of the firefly luciferase. Panels on the left show the bright-field
images and panels on the right show the corresponding luminescence images. Split-luciferase assay testing the ability of INP1 and INP2 from
Arabidopsis and tomato to interact with a protein from another species. Only the same-species interactions were observed. — Figure 6c. Split-luciferase assay testing the ability of SlINP1 and SlINP2 to interact. Tobacco leaves were divided into sectors co-expressing indicated proteins containing the N-terminal (NLuc) and C-terminal (CLuc) parts of the firefly luciferase. Panels on the left show the bright-field images and panels on the right show the corresponding luminescence images. Split-luciferase assay testing the ability of INP1 and INP2 from Arabidopsis and tomato to interact with a protein from another species. Only the same-species interactions were observed.

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NIR Fluorescence Imaging

Western Blots
In-cell Western
On-cell Western

Accumulation and persistence of ivacaftor in airway epithelia with prolonged treatment

Tara N. Guhr Lee, Deborah M. Cholon, Nancy L. Quinney, Martina Gentzsch, Charles R. Esther Jr

…Blots were probed with mouse monoclonal anti-CFTR antibodies and then with IRDye 680– goat anti-mouse immunoglobulin G (Molecular Probes). Anti-actin (Cell Signaling) was used as a loading control. Protein bands were visualized using a Sapphire Biomolecular Imager (Azure Biosystems)…

Cyst Fibros. 2020;19(5):746-751

Differential influence of Streptococcus mitis on host response to metals in reconstructed human skin and oral mucosa

Lin Shang, Dongmei Deng, Sanne Roffel, and Susan Gibbs

tern Blots Fig. 6. Toll-like receptor (TLR) protein expression in reconstructed human skin (RHS) and gingiva (RHG). TLR1 and 4 proteins are shown together with reference tubulin expression. TLR2, 3, 5, and 6 were under the detectable level (data now shown). Data are representative of three independent experiments ee times in PBST d dye-conjugated use (1:7500 for :7500 for TLR1, 2, blots were ecular Imager rnia).…” WESTERN BLOTS Differential influence of Streptococcus …membranes were washed three times in PBST and further incubated with infrared dyeconjugated secondary antibodies against mouse (1:7500 for TLR3, 4, or 5) or against rabbit (1:7500 for TLR1, 2, 6, or tubulin). After washing, the blots were visualized using Sapphire Biomolecular Imager (Azure biosystems, Dublin California)…

Contact Dermatitis. 2020;83(5):347–360

Alboserpin, the Main Salivary Anticoagulant from the Disease Vector Aedes albopictus, Displays Anti–FXa-PAR Signaling In Vitro and In Vivo

Gaurav Shrivastava, Paola Carolina Valenzuela-Leon, Andrezza Campos Chagas, Olivia Kern, Karina Botello, Yixiang Zhang, Ines Martin-Martin, Markus Berger Oliveira, Lucas Tirloni, and Eric Calvo

…Finally, the plates were scanned at 700 and 800 nm, and the intensity of the labeled proteins was measured using the Azure sapphire biomolecular imager (Azure Biosystems)

Immunohorizons.2022;6(6):373–383

Read about On-Cell Western

Phosphor Imaging

Mobility Shift Assays
RNA Gels
Southern Blots
Tissue Imaging

High Dose IFN-β Activates GAF to Enhance Expression of ISGF3 Target Genes in MLE12 Epithelial Cells

Kensei Kishimoto, Catera L. Wilder, Justin Buchanan, Minh Nguyen, Chidera Okeke, Alexander Hoffmann and Quen J. Cheng

…Nuclear extracts…were incubated with P32-labaled oligonucleotide probes…The reaction mixtures were run on a 5% acrylamide (30:0.8) gel with 5% glycerol and TGE buffer (24.8 mM Tris, 190 mM glycine, 1 mM EDTA) at 200V for 1 hour and 45 mins. The gels were dried and imaged on a Sapphire Biomolecular imager in phosphor mode (Azure Biosystems, Dublin, CA).

Front Immunol. 2021;12:651254

Engineered Viral RNA Decay Intermediates to Assess XRN1-mediated decay

Joseph Russo, Cary T. Mundell, Phillida A. Charley, Carol Wilusz, and Jeffrey Wilusz

…The samples were then resolved via denaturing PAGE, dried, exposed to a phosphor screen, and viewed via an Azure Sapphire Biomolecular Imager providing sufficient sensitivity to observe all required bands with one exposure.

Methods. 2019;155:116–123

Figure 4. Quantification/graphical representation
of gels presented in vitro decay assays and xrRNA
readouts of selected RNA substrates. Using the
representative data obtained in Fig. 3, the relative
levels of the full length input RNA (Input circles) or the
xrRNA reporter decay intermediate (xrRNA squares)
for GAPDH CDS and GAPDH CDS-BNYVV were
quantified by phosphorimaging using pixel counting.
Results are presented as the amount of the indicated
RNA species relative to the band/lane of the gel with
the maximal pixel count for each RNA. — Figure 4. Quantification/graphical representation of gels presented in vitro decay assays and xrRNA readouts of selected RNA substrates. Using the representative data obtained in Fig. 3, the relative levels of the full length input RNA (Input circles) or the xrRNA reporter decay intermediate (xrRNA squares) for GAPDH CDS and GAPDH CDS-BNYVV were quantified by phosphorimaging using pixel counting. Results are presented as the amount of the indicated RNA species relative to the band/lane of the gel with the maximal pixel count for each RNA.

Host cell-dependent late entry step as determinant of hepatitis B virus infection

Xupeng Hong, Yuka Imamura Kawasawa, Stephan Menne, Jianming Hu

…Viral DNAs were resolved on 1.2% agarose gel and detected by 32P-labeled HBV or WHV DNA probes…DNA signals from Southern blot analysis were detected by Sapphire Biomolecular Imager (Azure Biosystems) and quantified using the Image Lab system 6.0.1 (Bio-Rad).

PLoS Pathog. 2022;18(6):e1010633

Figure 4. WCH-17 cells were rendered susceptible to HBV infection after huNTCP expression. WCH-17 (A, B) or WC3 (C, D) parental and
huNTCP-expressing cells were plated on regular culture dishes (i.e., with no collagen coating) and infected with ca. 400 genome equivalent
of HBV per cell. Three days post infection, the PF DNA (i.e., Hirt DNA) from mock- or HBV-infected cells was extracted by Hirt extraction and
treated with Exo I/III followed by Southern blot analysis. Hirt DNA from HBV-infected HepG2-huNTCP cells, loaded at 4-fold less than the Hirt
DNA from woodchuck cells, served as the positive control for cccDNA detection. — Figure 4. WCH-17 cells were rendered susceptible to HBV infection after huNTCP expression. WCH-17 (A, B) or WC3 (C, D) parental and huNTCP-expressing cells were plated on regular culture dishes (i.e., with no collagen coating) and infected with ca. 400 genome equivalent of HBV per cell. Three days post infection, the PF DNA (i.e., Hirt DNA) from mock- or HBV-infected cells was extracted by Hirt extraction and treated with Exo I/III followed by Southern blot analysis. Hirt DNA from HBV-infected HepG2-huNTCP cells, loaded at 4-fold less than the Hirt DNA from woodchuck cells, served as the positive control for cccDNA detection.

Preclinical evaluation of [18F]FDG‐PET as a biomarker of lymphoid tissue disease and inflammation in Zika virus infection

Carla Bianca Luena Victorio, Joanne Ong, Jing Yang Tham, Marie Jennifer Reolo, Wisna Novera, Rasha Msallam, Satoru Watanabe, Shirin Kalimuddin, Jenny G. Low, Subhash G. Vasudevan, Ann‐Marie Chacko

…Infected mice were injected i.v. with 10 MBq [18 F]FDG and tissues were harvested following a 60-min tracer uptake. Freshly isolated wholemount lymphoid tissues were immediately exposed to multi-purpose phosphoscreen (BAS-IP MS) for 30 min. Fresh tissues with high tracer uptake were exposed to super-resolution (BAS-IP SR) phosphoscreen for 5 min (GE Healthcare Life Sciences, USA). [ 8 F]FDG standards at 2/3 serial dilution from 600 to 0 kBq were mounted together with mouse tissue for calibration of digital autoradiography (DAR) images. Screens were then scanned using the Sapphire Biomolecular Imager (Azure Biosystems, USA) at 100-μm resolution…

Eur J Nucl Med Mol Imaging. 2022;49(13):4516-4528

Thin Layer Chromatography

Identification and functional analysis of a galactosyltransferase capable of cholesterol glycolipid formation in the Lyme disease spirochete Borrelia burgdorferi

Petronella R. Hove, Forgivemore Magunda, Maria Angela de Mello Marques, M. Nurul Islam, Marisa R. Harton, Mary Jackson, John T. Belisle

…The lipid extracts were resolved by thin-layer chromatography (TLC) using silica gel G60 TLC plates and chloroform/methanol (90:10 v/v) as the mobile phase. Reference non-radioactive standards were resolved concurrently and developed separately from the blot containing radioactive lipids with phosphomolybdic acid stain. Following this, blot with reference standard was matched with TLC blot to mark resolution of lipids. After development radiolabeled lipids were imaged using Azure Sapphire Biomolecular Imager (Azure Biosystems Inc, Dublin, CA)…

PLoS One. 2021;16(6):e0252214

Figure 5. TLC of lipids from cell free assays for the
enzymatic incorporation of [26- 14C] cholesterol into
cholesteryl-β-D-galacto-pyranoside (CGal). A. The
formation of cholesteryl-β-D-galactopyranoside (CGal)
(arrow) in whole cell lysates of Bb and recombinant E.
coli expressing WT bb0572 (lane 1 and 2). As expected,
CGal was not formed by the whole cell lysate of E. coli
expressing mutant bb0572 ΔCFF/I/DGD (lane 3). Lane 4
is the empty expression vector control, and lane 6 and 7
are from boiled whole cell lysates of Bb and recombinant
E. coli expressing WT bb0572, respectively. Note lane 5
was not used. B. Substrate controls: Bb and E. coli WT
bb0572 whole cell lysates with UDP-Gal (lane 1 and 2);
GDP-Man (lanes 3 and 4), or cholesterol oxidase treated
[26- 14C] cholesterol with UDP-Gal (lane 5 and 6) as
substrate. CHO, cholesterol; ACGal, cholesteryl 6-O-acylβ-D-galactopyranoside and MGal, monogalactosyl
diacylglycerol. Ec WT (Escherichia coli wild type), Ec MT
(E.coli mutant—bb0572 ΔCFF/DGD/I) and Ec VC- E.coli
vector control). — Figure 5. TLC of lipids from cell free assays for the enzymatic incorporation of [26- 14C] cholesterol into cholesteryl-β-D-galacto-pyranoside (CGal). A. The formation of cholesteryl-β-D-galactopyranoside (CGal) (arrow) in whole cell lysates of Bb and recombinant E. coli expressing WT bb0572 (lane 1 and 2). As expected, CGal was not formed by the whole cell lysate of E. coli expressing mutant bb0572 ΔCFF/I/DGD (lane 3). Lane 4 is the empty expression vector control, and lane 6 and 7 are from boiled whole cell lysates of Bb and recombinant E. coli expressing WT bb0572, respectively. Note lane 5 was not used. B. Substrate controls: Bb and E. coli WT bb0572 whole cell lysates with UDP-Gal (lane 1 and 2); GDP-Man (lanes 3 and 4), or cholesterol oxidase treated [26- 14C] cholesterol with UDP-Gal (lane 5 and 6) as substrate. CHO, cholesterol; ACGal, cholesteryl 6-O-acylβ-D-galactopyranoside and MGal, monogalactosyl diacylglycerol. Ec WT (Escherichia coli wild type), Ec MT (E.coli mutant—bb0572 ΔCFF/DGD/I) and Ec VC- E.coli vector control).

White Light Imaging

Lateral Flow Immunoassay Development
Clonogenic Assay

A novel rapid detection for SARS-CoV-2 spike 1 antigens using human angiotensin converting enzyme 2 (ACE2)

Jong-Hwan Lee, Minsuk Choi, Yujin Jung, Sung Kyun Lee, Chang-Seop Lee, Jung Kim, Jongwoo Kim, Nam Hoon Kim, Bum-Tae Kim, Hong Gi Kim

…the intensity of the test and control lines were converted to peak histograms using a Sapphire Biomolecular Imager.

Biosens Bioelectron. 2021;171:112715

Figure 4. Identification of the sandwich pair for detection of
SARS-CoV-2 spike antigen. a) Schematic diagram of LFIA
using ACE2 as the capture probe and sandwich analysis
results obtained from paired antibodies (CR3022, F26G19,
and S1mAb). SARS-CoV-2 S1 antigen (50 ng) was used as a
positive control, and buffer containing no S1 antigen was used
as a negative control. After 20 min, the strips were captured
by a smartphone, and their peak intensities were analyzed. b)
Schematic diagram of LFIA, using antibodies as the capture
probe, and their sandwich analysis results. c) Peak intensities
of capture probe (PC)–detection probe (PD) pairs. A total of 12
pairs of positive controls (50 ng S1 antigen) were tested, and
their intensities were analyzed. Peak intensity was calculated
by subtracting the background intensity of the strip from the
average intensities of the dots. — Figure 4. Identification of the sandwich pair for detection of SARS-CoV-2 spike antigen. a) Schematic diagram of LFIA using ACE2 as the capture probe and sandwich analysis results obtained from paired antibodies (CR3022, F26G19, and S1mAb). SARS-CoV-2 S1 antigen (50 ng) was used as a positive control, and buffer containing no S1 antigen was used as a negative control. After 20 min, the strips were captured by a smartphone, and their peak intensities were analyzed. b) Schematic diagram of LFIA, using antibodies as the capture probe, and their sandwich analysis results. c) Peak intensities of capture probe (PC)–detection probe (PD) pairs. A total of 12 pairs of positive controls (50 ng S1 antigen) were tested, and their intensities were analyzed. Peak intensity was calculated by subtracting the background intensity of the strip from the average intensities of the dots.

Figure 6b. Laboratory confirmation of ACE2-based LFIA
using clinical samples. b) Results of ACE2-based LFA for the
detection sensitivity of cultured SARS-CoV-2. Serially diluted
virus concentrates (concentration range: 1.07 × 108 copies/
mL to 5.35 × 106 copies/mL) were tested. After 20 min, the
LFIA strip was taken with a smartphone and scanned with an
image analyzer. The line intensities of the test and control lines
were converted to peak histograms. Also, the intensity of the
test lines was measured by a portable line analyzer (IL: line
intensity of test line). Furthermore, human coronavirus (OC43)
was tested as a negative control. — Figure 6b. Laboratory confirmation of ACE2-based LFIA using clinical samples. b) Results of ACE2-based LFA for the detection sensitivity of cultured SARS-CoV-2. Serially diluted virus concentrates (concentration range: 1.07 × 108 copies/ mL to 5.35 × 106 copies/mL) were tested. After 20 min, the LFIA strip was taken with a smartphone and scanned with an image analyzer. The line intensities of the test and control lines were converted to peak histograms. Also, the intensity of the test lines was measured by a portable line analyzer (IL: line intensity of test line). Furthermore, human coronavirus (OC43) was tested as a negative control.

A novel rapid detection for SARS-CoV-2 spike 1 antigens using human angiotensin converting enzyme 2 (ACE2)

Jong-Hwan Lee, Minsuk Choi, Yujin Jung, Sung Kyun Lee, Chang-Seop Lee, Jung Kim, Jongwoo Kim, Nam Hoon Kim, Bum-Tae Kim, Hong Gi Kim

…The clonogenic recovery potential gives insight into the capability of the cells to form a new tumor (metastasis). Therefore, 500 cells/well were seeded on a 6-well-plate and treated for 10 days with ART. Untreated cells served as controls. RCC cells were subsequently fixed with 85% MeOH/15% AcOH and stained with Coomassie (0.5 g Coomassie Blue G250 (SigmaAldrich, Darmstadt, Germany), 75 mL AcOH, 200 mL MeOH, 725 mL distilled water). Amount and size of cell clone colonies were measured with a biomolecular imager (Sapphire, Azure Biosystems, Biozym, Hess. Oldendorf, Germany)…

Cancers. 2020;12(11):3150

Applications

Recently Published Research

Azure Imagers have been published in over 2,000 publications worldwide. Our high-resolution CCD cameras are the reason behind reproducible data and publication-ready images.

Impressed with the Sapphire FL and want to quickly share relevant publications with a colleague? Download a free booklet with publications featuring the SFL here.

Specifications

Parameter	Sapphire FL
Detection modes	Fluorescence, phosphor imaging, densitometry, and chemiluminescence*
Detectors	Confocal scanning with lasers and PMT or APD detectors
Pixel size	5, 10, 20, 25, 50, 75, 100, 150, 170, 200, 500, 1000 μm
Supported wavelength range	375 – 900 nm
Excitation range	375 – 850 nm
Emission range	380 – 900 nm
Maximum field of view (FOV):	25 cm x 25 cm
Z-plane focal range	7 mm; minimum delta 0.01mm, from -1 mm below the glass to 6 mm above the glass
Standard optical modules	Solid-state laser diodes emitting at 375, 450, 488, 532, 638, 658, 685, 730, and 784 nm. Phosphor Standard Optical Module available.
Custom Optical Modules	Custom lasers and filters are available upon request to provide exceptional research flexibility.
Wide dynamic range	24-bit
Clearance from platen to lid	4 cm
Animal imaging	Yes, with 5 built-in anesthesia ports compatible with standard small animal anesthesia tubing
Unit dimensions (W x H x D)	63.0 cm x 39.9 cm x 59.3 cm / 24.8” x 15.7” x 23.3"
Weight	43.5 kg / 95.9 lbs
Power requirements	100 – 240 VAC ± 10%, 50/60 Hz
Connection to computer	Fast LAN (Ethernet) cable
Certifications	CE, cTUVus, CB scheme

*Chemiluminescence module sold separately

Resources

Brochure

Document Type	Description
Video	How the Sapphire FL Supports In Vitro to In Vivo Research	WATCH
Brochure	Sapphire FL Biomolecular Imager Family Brochure	DOWNLOAD
Booklet	Sapphire FL Biomolecular Imager Applications and Publications	DOWNLOAD

Application Notes

Title	Link
Imaging mCherry and tdTomato Expressing Cells with the Sapphire FL	Download
Evaluating Keratinocytes' Viability After UVB Exposure on the Sapphire Biomolecular Imager	Download
Imaging Thick-Sectioned Slides with the SFL	Download
In-gel fluorescence optimization for GFP-labeled protein purification	Download
CD44 Determination by Western Blot	Download
Near infrared fluorescent detection of a human cytokine array	Download
Mitochondrial function evaluation on UVB-exposed keratinocytes measured using fluorescent probes	Download
Imaging CFP-expressing Cells with the Sapphire FL	Download
Scanning Microarrays with the Sapphire FL Biomolecular Imager	Download
Easy Screening of Autophagy-Associated Acidic Compartments	Download
Biocompare Bench Tip: How In-Cell Westerns Can Advance Your Research	Download
Sapphire FL’s Laser and Filter Flexibility Allows Imaging of Dyes with Large Stokes Shifts	Download
Biomolecular Fluorescence Complementation (BiFC) Assay for Determination of in-vitro Protein-Ligand Interaction	Download
Imaging of Multiplex Fluorescent Immunohistochemistry Slides	Download
Using Total Cell Staining and Advanced Imaging to Unlock the Full Power of In-Cell Westerns	Download
Imaging YFP-expressing cells	Download
Multicolor Fluorescent Imaging of Cultured Cells on the Sapphire FL Biomolecular Imager: Live/Dead (AO/PI) Assays	Download
In vivo imaging and monitoring of RFP+ tumors in mice	Download
Fluorescent IHC Imaging of Tumor Tissue Arrays	Download
Phosphor Imaging with Sapphire	Download
Increasing Assay Efficiency with Four Color Detection	Download
In-cell Western Blotting	Download

Shop Accessories

Sapphire Eraser Storage Phosphor Screen Image Eraser

Slide Holder

Plate Holder

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Product Inquiry

See what the hype is about. Let's chat.

What Scientists Say about the Sapphire FL

Our lab recently acquired a Sapphire FL Biomolecular Imager. This imager is a game-changer, and the analysis software is fantastic. Going from regularly using an imager built in the 90’s to a unit this modern is a substantial leap forward, but this overdue upgrade in machinery is not the sole reason for our excitement. Its sensitivity, resolution, and user-friendly UI speak volumes for the quality of the product and has made imaging an even more enjoyable part of our experiments. My colleagues and I are looking forward to using it for… more

Ryan Steere

Graduate Student, Ohio University

I love the Sapphire! It completely changed my process to simplify acquiring top quality by multiplexing in FOUR channels to achieve publishable data every time. The secondary antibodies were incredible. I had so many ideas for how to use the flat bed to multiplex our assays on plates and really integrate protein recycling.

Melissa Fernandez, PhD

Federal Research Fellow, NCI - Ft. Detrick

My lab has a Sapphire Imager RGB-NIR with 4 lasers for over a year and a half now. This has been a workhorse for us as we use it for fluorescent gels and western blots regularly. My researchers have been happy with it and I am pretty happy with the imager.

Allen Liu

We have started using the Azure Sapphire FL and are loving it. We have it equipped for phosphor and fluorescence imaging. We love the interoperability, ease of use, and ability to acquire fluorescence from gels while they are still between glass plates.

Jeff Dahlseid

For Research Use Only

Headquarters:
Azure Biosystems, Inc.
6747 Sierra Court, Suite A-B
Dublin, CA 94568
United States

Tel: (925) 307 7127
Fax: (925) 905-1816
Info@azurebiosystems.com

Azure Biosystems Pte. Ltd.
68 Circular Road, #02-01
Singapore, 049422

Copyright © 2026 Azure Biosystems Inc.

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Sapphire FL Biomolecular Imager

IS4000

Unmatched Application Versatility and Performance

24-bit Dynamic Range

5 Micron Resolution

Custom Design Lasers and Filters

Adjustable focus for sample thickness

Key Features

Unlimited Laser and Filter Wavelength combinations

Application Flexibility

Sensitive Fluorescent Detection

Total Flexibility with Phosphor Imaging

In Vivo Imaging

Distinguish between Subtle Differences in Expression with Extended Dynamic Range (EDR)

Adjustable Focus

5-micron Resolution

Chemiluminescent Imaging When You Need It

Frequently Asked Questions

Trusted by Scientists Worldwide

Applications

Fluorescence Imaging

Tissue Imaging

In-Gel Imaging

White Light and Autofluorescence

Rescue of autoimmune hepatitis by soluble MHC class II molecules in an altered concanavalin A-induced experimental model

Immunohistochemistry

The ISL LIM-homeobox 2 transcription factor is negatively regulated by circadian adrenergic signaling to repress the expression of Aanat in pinealocytes of the rat pineal gland

J Pineal Res. 2023;e12905

Parallel imaging of coagulation pathway proteases activated protein C, thrombin, and factor Xa in human plasma

Method for the elucidation of LAMP products captured on lateral flow strips in a point of care test for HPV 16

Proteomic Analysis of Endometrial Cancer Tissues from Patients with Type 2 Diabetes Mellitus

Gel Shift Assay

Organ Imaging

(example of DNA-Protein Binding Assay)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing

Visible Fluorescence

Neutrophils incite and macrophages avert electrical storm after myocardial infarction

Visible Fluorescence

Eliminating mesothelioma by AAV-vectored, PD1-based vaccination in the tumor microenvironment

ZnO Nanowire-Based Early Detection of SARS-CoV-2 Antibody Responses in Asymptomatic Patients with COVID-19

Chemiluminescence Imaging

Western Blots

Protein Arrays

Plant Leaf Bioluminescence

Shikonin Reduces Growth of Docetaxel-Resistant Prostate Cancer Cells Mainly through Necroptosis

Cancers. 2021;13(4):882

NonO Is a Novel Co-factor of PRDM1 and Regulates Inflammatory Response in Monocyte Derived-Dendritic Cells

Front Immunol. 2020;11:1436

Effects of prolactin on ventricular myocyte shortening and calcium transport in the streptozotocin-induced diabetic rat

Heliyon. 2020;6(4):e03797

A dominant negative mitofusin causes mitochondrial perinuclear clusters because of aberrant tethering

Engineered unnatural ubiquitin for optimal detection of deubiquitinating enzymes

Chem Sci. 2020;11(23):6058-6069

In vitro Evaluation of ASCs and HUVECs Co-cultures in 3D Biodegradable Hydrogels on Neurite Outgrowth and Vascular Organization

A species-specific functional module controls formation of pollen apertures

NIR Fluorescence Imaging

Western Blots

In-cell Western

Accumulation and persistence of ivacaftor in airway epithelia with prolonged treatment

Cyst Fibros. 2020;19(5):746-751

Differential influence of Streptococcus mitis on host response to metals in reconstructed human skin and oral mucosa

Alboserpin, the Main Salivary Anticoagulant from the Disease Vector Aedes albopictus, Displays Anti–FXa-PAR Signaling In Vitro and In Vivo

Phosphor Imaging

Mobility Shift Assays

RNA Gels

Southern Blots

Tissue Imaging

High Dose IFN-β Activates GAF to Enhance Expression of ISGF3 Target Genes in MLE12 Epithelial Cells

Engineered Viral RNA Decay Intermediates to Assess XRN1-mediated decay

Host cell-dependent late entry step as determinant of hepatitis B virus infection

Preclinical evaluation of [18F]FDG‐PET as a biomarker of lymphoid tissue disease and inflammation in Zika virus infection

Thin Layer Chromatography

Identification and functional analysis of a galactosyltransferase capable of cholesterol glycolipid formation in the Lyme disease spirochete Borrelia burgdorferi

White Light Imaging

Lateral Flow Immunoassay Development

Clonogenic Assay

A novel rapid detection for SARS-CoV-2 spike 1 antigens using human angiotensin converting enzyme 2 (ACE2)

A novel rapid detection for SARS-CoV-2 spike 1 antigens using human angiotensin converting enzyme 2 (ACE2)

Applications

Recently Published Research