Azure Cielo qPCR system

Azure Cielo™ Real-time PCR system

Fast qPCR detection system for high-quality data with smart protocols, and robust durability

Experts Trust the Cielo qPCR System

What Scientists Say about the Cielo

"Easy, accurate RT PCR machine with great software."

5/5

As a lab that has been carrying out high volume PCR testing for the last 12 months, the Azure Cielo Real-Time PCR machine has been a fantastic addition to our suite of equipment to facilitate this process. The comparison data we have achieved… more

"Super real time PCR."

5/5

It is a very user friendly instrument. A very rapid thermocycler, with very good reproducibility. A good instrument to scale and increase produtivity. We have 3 in our lab and I really recomend this real time PCR system for diferent aplications… more

Azure Cielo Benefits

connectivity

Email Notifications


Finally, qPCR data can be sent directly to your inbox after a run without cables!
Transfer data via Ethernet, Wi-Fi, or USB.

flexibility

Flexible

 

Engineered for a wide variety of qPCR applications with high sensitivity and wide dynamic range.​

workflow

Intelligent Workflow

 

User interface allows simple touchscreen assay setup. Saved protocols make assay set up easy and reproducible.

reliability

Reliable and Robust

 

Optics and thermal block are designed to deliver the same uniformity and reproducibility to last for up to 1,000 experiments and beyond.

Key Features

Email Notifications

Starting a run at the end of the day but need your data soon? Automatic email notifications from Cielo Manager have you covered.

Because the Cielo connects via Wi-Fi, this feature allows its software to not only notify you when your run is completed, but to also send the data straight to your inbox.

Email notification shown after qPCR run on Azure Cielo qPCR system
Receive end-of-experiment notifications and data files via email from the Cielo.

Quick Set up and Plate Layout Confirmations

Each well is easily labeled with three identifiers:

  • Well type,
  • Sample ID, and
  • Dye/target.

The identifiers are used for data analysis and easy visualization of the amplification and standard curves.

Plate layout on Azure Cielo Manager Software
Each well is color coded by sample type, biological replicate, and technical replicate. Well type and standard curve values are also easily visible.

View Amplification Trace Individually by Target or Sample

Easily view all of your data at once or select specific wells/targets to view a subset of your data. Cielo’s software allows you to easily adjust threshold values by dragging the threshold line, or typing in the threshold value.

You can visualize amplification curves in linear or log view, and change the color of the amplification curves to easily distinguish the data by Sample ID or target.

5-point, 10-fold dilution series of a six-target qPCR assay. Targets: RRP36 (Efficiency = 96.8%, R2 = 0.99), ACTB (Efficiency = 97.1%, R2 = 0.99), SDHA (Efficiency = 98.0%, R2 = 0.99), GAPDH (Efficiency = 100.8%, R2 = 0.99), TBP (Efficiency = 95.5%, R2 = 0.99), and B2M (Efficiency = 97.5%, R2 = 0.99).

Quickly Understand Data

Quickly review Cq values, and assess data uniformity and separation between dilutions.

The wells shown here are colored in a 2D Sample Heat Map based on Cq value, with the lowest Cq values in green and highest Cq values in red.

qPCR heatmap using Azure Cielo Manager
7-point, 10-fold dilution series in duplicate amplifying a 150 bp amplicon (Efficiency = 94.3%, R2 = 0.99), with two unknowns, each with four replicates.

Optical Design Eliminates the Need for ROX

Well-based excitation and color-specific LED light sources ensure the excitation efficiency in every well for every channel is the same.

Coupled with scanning technology that collects multiple data points from each well, the Cielo’s well-based optical design eliminates the need for normalization dyes, such as ROX. Rather than a single, stationery light source and detector system, the Cielo utilizes 16-well simultaneous excitation and signal detection that ensures uniform data acquisition.

qPCR amplification curves using Azure Cielo Manager
Amplification curves for 96 replicates of the same 150 bp amplicon (Mean Cq = 19.5, Cq Standard deviation = 0.03).

100,000 Data Points per Well

The Cielo is able to capture approximately 100,000 pixels/wells, enabling accurate, reproducible and sensitive representation of fluorescent intensities from each qPCR well.

Competitive systems are only able to acquire 1 pixel/well.

Single copy amplification was detected in 88 wells (92%) of the plate run on the Cielo, and in 77 wells (80%) on the plate run on the competitor system.

Cielo is able to capture approximately 100,000 pixels per qPCR well
Single illumination light sources can deliver light both in and out of the wells, resulting in higher background, and less light hitting the sample. The Cielo uses fiber optics to directly deliver light to each individual well.

Engineered for Reproducibility

High-quality engineering includes Peltier-based thermal block system designed for superior thermal cycling and unmatched optical design to ensure every run is accurate and reproducible.

The Cielo is able to demonstrate reproducible quantitation down to a single copy of the target sequence.

Amplification curves for 96 replicates shown on a linear plot
Unparalleled uniformity. Amplification curves for 96 replicates of the same 150 bp amplicon: A. Linear view, B. Log view. (Mean Cq = 19.1, Cq Standard deviation = 0.001).

Six-target Multiplex

Multiplex up to six targets on the Cielo, a true six-channel system. Flexibility is built into the system for the use of numerous commercially available dyes and applications.

Because ROX is not required, it can also be used as a probe.

Azure Cielo Manager software showing six-target multiplexing
8-point, 10-fold standard curves for six targets. Targets: RRP36 (Efficiency = 103.6%, R2 = 0.96), ACTB (Efficiency = 100.5%, R2 = 0.99), RNAse P (Efficiency = 97.0%, R2 = 0.99), GAPDH (Efficiency = 100.2%, R2 = 1.00), TBP (Efficiency = 96.8%, R2 = 0.99), and EF1a (Efficiency = 97.2%, R2 = 0.99).

Choose Between Models

ParameterCielo 6 (AIQ060)Cielo 3 (AIQ030)
Sample capacity (Wells)96 wells96 wells
Compatible Plates/Tubes96-well plates and tubes, 8- or 12-well strips and single tubes96-well plates and tubes, 8- or 12-well strips and single tubes
Reaction Volume1-150 uL (10-65 uL recommended)1-150 uL (10-65 uL recommended)
Dynamic Range10 Logs10 Logs
Excitation SourceLED Excitation range: 450-730 nmLED Excitation range: 450-730 nm, 610-700 nm
Detection Channels63
MultiplexingUp to 6 targetsUp to 3 targets
Reaction Temperature4°C to 99.9°C4°C to 99.9°C
Max Block Ramp Rate6°C/sec6°C/sec
Average Sample Ramp Rate4°C/Sec4°C/Sec
Temperature Uniformity±0.2°C±0.2°C
Temperature Accuracy±0.1°C±0.1°C
Custom Dye/Chemistry
Onboard Memory32 GB Equivalent to 20,000 experiments32 GB Equivalent to 20,000 experiments
Unit dimensions (W x H x D)​30.5 cm x 50.8 cm x 43.2 cm / 12” x 20” x 17"30.5 cm x 50.8 cm x 43.2 cm / 12” x 20” x 17"
Weight​22.7kg / 50 lbs22.7kg / 50 lbs
Power requirements100-240 VAC, 8A; Consumption: 0.8kW (800 Watts)100-240 VAC, 8A; Consumption: 0.8kW (800 Watts)
ConnectivityUSB, Wifi, EthernetUSB, Wifi, Ethernet
CertificationsCE Certified, TUV CertifiedCE Certified, TUV Certified

Compare Cielo Models (Dyes/Probes)

Dyes/ProbesCielo 6 (AIQ060)Cielo 3 (AIQ030)
• SYBR® Green • EvaGreen™ • FAM™
• VIC® • HEX™ • JOE™ • CAL Fluor® 540 • CAL Fluor® Orange 560
• TAMRA™ • Cy3
• ROX™ • TEX®615, • CAL Fluor® Red 610 • CXR
• Cy®5 • Quasar®670, • Liz® • Mustang Purple®
• Cy®5.5 • Quasar 705

Other dyes/probes are possible depending on the system configuration.

For In-vitro Diagnostics

Azure Cielo Dx

The Azure Cielo Dx qPCR system for IVD use brings you the accuracy and sensitivity you need for your research, with intuitive touch screen software. With 6-target detection, the flexibility you need is at your fingertips.

USA: For IVD use. The Azure Cielo 3 Dx and Azure Cielo 6 Dx Real-time PCR systems are registered with the U.S. FDA as Class II 510(k) exempt devices.

Europe:  For IVD use. The Azure Cielo 3 Dx and Azure Cielo 6 Dx systems are CE marked under the In Vitro Diagnostic Medical Devices Directive (98/79/EC). The CE IVD–authorized Azure Cielo Dx Systems are for distribution and use the following countries: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czechia, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Liechtenstein, Lithuania, Luxembourg, Malta, the Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, and the United Kingdom.

Applications

Recently Published Research

Resources

Document TypeDescription
BrochureCielo BrochureDOWNLOAD
App NoteUsing the Azure Cielo™ Real-Time PCR System for SARS-CoV-2 viral RNA detectionDOWNLOAD
App Note6-Channel Multiplex qPCR Using Azure Cielo Real-Time PCR SystemDOWNLOAD
App NoteSensitive and Accurate Single-Copy Amplicon Detection in Azure Cielo Real-Time PCR SystemDOWNLOAD
App NoteAzure Cielo™ Real-Time PCR Optical Design Eliminates the Need for Passive Reference DyeDOWNLOAD
App NoteWhat Sets the Cielo ApartDOWNLOAD
App NoteDetection of low copy telomeric genes with CieloDOWNLOAD
App NoteGoTaq Enviro Wastewater SARS-CoV-2DOWNLOAD
App NoteAzure Cielo 6 Compatibility with GenDx KMRtype and KMRtrack WorkflowsDOWNLOAD
Technical NoteSNP Genotyping/Allelic DiscriminationDOWNLOAD
Technical NoteAdding Dyes and Color CompensationDOWNLOAD
App NotePerformance of a six-target multiplexing assay on the Cielo™ 6 using the Quantabio PerfeCTa® Multiplex qPCR ToughMixDOWNLOAD
VideosHow to Use the CieloWATCH

Faster and more precise qPCR is within reach. Contact us today to learn more about the Azure Cielo.

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