Azure Biosystems
qPCR detection system for fast run times, high-quality data, smart protocols, and robust durability
As a lab that has been carrying out high volume PCR testing for the last 12 months, the Azure Cielo Real-Time PCR machine has been a fantastic addition to our suite of equipment to facilitate this process. The comparison data we have achieved… more
It is a very user friendly instrument. A very rapid thermocycler, with very good reproducibility. A good instrument to scale and increase produtivity. We have 3 in our lab and I really recomend this real time PCR system for diferent aplications… more
Starting a run at the end of the day but need your data soon? Automatic email notifications from Cielo Manager have you covered.
The Cielo’s connection via Wi-Fi allows its software to not only notify you when your run is completed, but sends the data straight to your inbox.
Each well is easily labeled with three identifiers:
These identifiers are used for data analysis and easy visualization of the amplification and standard curves.
Easily view all of your data at once or select specific wells/targets to view a subset of your data. Cielo’s software allows you to easily adjust threshold values by dragging the threshold line, or typing in the threshold value.
You can visualize amplification curves in linear or log view, and change the color of the amplification curves to easily distinguish the data by Sample ID or target.
Quickly review Cq values, and assess data uniformity and separation between dilutions. Wells are colored in a 2D Sample Heat Map based on Cq value, with the lowest Cq values in green and highest Cq values in red.
Well-based excitation and color-specific LED light sources ensure the excitation efficiency in every well for every channel is the same.
Coupled with scanning technology that collects multiple data points from each well, the Cielo’s well-based optical design eliminates the need for normalization dyes, such as ROX. Rather than a single, stationery light source and detector system, the Cielo utilizes 16-well simultaneous excitation and signal detection that ensures uniform data acquisition.
The Cielo is able to capture approximately 100,000 pixels/wells, enabling accurate, reproducible and sensitive representation of fluorescent intensities from each qPCR well.
Competitive systems are only able to acquire 1 pixel/well.
Single copy amplification was detected in 88 wells (92%) of the plate run on the Cielo Real-Time PCR system, and in 77 wells (80%) on the plate run on the competitor system.
High-quality engineering includes Peltier-based thermal block system designed for superior thermal cycling and unmatched optical design to ensure every run is accurate and reproducible.
The Cielo is able to demonstrate reproducible quantitation down to a single copy of the target sequence.
Multiplex up to six targets on the Cielo, a true six-channel system. Flexibility is built into the system for the use of numerous commercially available dyes and applications.
Because ROX is not required, it can also be used as a probe.
| Parameter | Cielo 6 (AIQ060) | Cielo 3 (AIQ030) |
|---|---|---|
| Sample capacity (Wells) | 96 wells | |
| Compatible Plates/Tubes | 96-well plates and tubes, 8- or 12-well strips and single tubes | |
| Reaction Volume | 1-50 uL (1-65 recommended) | |
| Dynamic Range | 10 Logs | |
| Excitation Source | LED Excitation range: 450-730 nm | LED Excitation range: 450-730 nm, 610-700 nm |
| Detection Channels | 6 | 3 |
| Multiplexing | Up to 3 targets | Up to 6 targets |
| Reaction Temperature | 4°C to 99.9°C | |
| Max Block Ramp Rate | 6°C/sec | |
| Average Sample Ramp Rate | 4°C/Sec | |
| Temperature Uniformity | ±0.2°C | |
| Temperature Accuracy | ±0.1°C | |
| Custom Dye/Chemistry | ✓ | |
| Onboard Memory | 32 GB Equivalent to 20,000 experiments | |
| Unit dimensions (W x H x D) | 30.5 cm x 50.8 cm x 43.2 cm / 12” x 20” x 17" | |
| Weight | 22.7kg / 50 lbs | |
| Power requirements | 100-240 VAC, 8A; Consumption: 0.8kW (800 Watts) | |
| Connectivity | USB, Wifi, Ethernet | |
| Certifications | CE Certified, TUV Certified | |
SAMPLE CAPACITY (WELLS)
96
COMPATIBLE PLATES/TUBES
96-WELL PLATES & TUBES AND 8
OR 12-WELL STRIPS & SINGLE TUBES
REACTION VOLUME
1-150 uL (10-65 uL recommended)
DYNAMIC RANGE
10 LOGS
EXCITATION SOURCE
LED, EXCITATION RANGE: 450–730NM
DETECTION CHANNELS
6
MULTIPLEXING
UP TO 6 TARGETS
REACTION TEMPERATURE
4°C to 99.9°C
MAX. BLOCK RAMP RATE
6°C/sec
AVG. SAMPLE RAMP RATE
4°C/SEC
TEMPERATURE UNIFORMITY
±0.2°C
TEMPERATURE ACCURACY
±0.1°C
CUSTOM DYE/CHEMISTRY
✓
ONBOARD MEMORY
32GB EQUIVALENT TO 20,000 EXPERIMENTS
CONNECTIVITY
USB, WI-FI, ETHERNET
FOOTPRINT (W/D/H)
12″ X 20″ X 17″ (30.5 X 50.8 X 43.2 CM)
SAMPLE CAPACITY (WELLS)
96
COMPATIBLE PLATES/TUBES
96-WELL PLATES & TUBES AND 8
OR 12-WELL STRIPS & SINGLE TUBES
REACTION VOLUME
1-150 uL (10-65 uL recommended)
DYNAMIC RANGE
10 LOGS
EXCITATION SOURCE
LED, EXCITATION RANGE: 450–580NM AND 610–700NM
DETECTION CHANNELS
3
MULTIPLEXING
UP TO 3 TARGETS
REACTION TEMPERATURE
4°C to 99.9°C
MAX. BLOCK RAMP RATE
6°C/sec
AVG. SAMPLE RAMP RATE
4°C/SEC
TEMPERATURE UNIFORMITY
±0.2°C
TEMPERATURE ACCURACY
±0.1°C
CUSTOM DYE/CHEMISTRY
✓
ONBOARD MEMORY
32GB Equivalent to 20,000 EXPERIMENTS
CONNECTIVITY
USB, WI-FI, ETHERNET
FOOTPRINT (W/D/H)
12″ X 20″ X 17″ (30.5 X 50.8 X 43.2 CM)
| Dyes/Probes | Cielo 6 (AIQ060) | Cielo 3 (AIQ030) |
|---|---|---|
| • SYBR® Green • EvaGreen™ • FAM™ | ✓ | ✓ |
| • VIC® • HEX™ • JOE™ • CAL Fluor® 540 • CAL Fluor® Orange 560 | ✓ | ✓ |
| • TAMRA™ • Cy3 | ✓ | |
| • ROX™ • TEX®615, • CAL Fluor® Red 610 • CXR | ✓ | |
| • Cy®5 • Quasar®670, • Liz® • Mustang Purple® | ✓ | ✓ |
| • Cy®5.5 • Quasar 705 | ✓ |
CHANNEL 1
EX: 475 ± 14
EM: 524 ± 12
DYES: SYBR™ Green, EvaGreen™, FAM™
CHANNEL 2
EX: 527 ± 10
EM: 565 ± 12
DYES: VIC®, HEX™, JOE™, CAL Fluor® 540, CAL Fluor®, Orange 560
CHANNEL 3
EX: 572 ± 7.5
EM: 623 ± 12
DYES: TAMRA™, Cy3
CHANNEL 4
EX: 537 ± 13
EM: 583 ± 11
DYES: ROX™, TEX®615, CAL Fluor® Red 610
CHANNEL 5
EX: 623 ± 12
EM: 676 ± 18.5
DYES: CY®5, Quasar®670, Liz®, Mustang Purple®
CHANNEL 6
EX: 655 ± 7.5
EM: 711 ± 12.5
DYES: CY® 5.5, Quasar 705
CHANNEL 1
EX: 475 ± 14
EM: 524 ± 12
DYES: SYBR™ Green, EvaGreen™, FAM™
CHANNEL 2
EX: 527 ± 10
EM: 565 ± 12
DYES: VIC®, HEX™, JOE™, CAL Fluor® 540, CAL Fluor®, Orange 560
CHANNEL 3
NOT AVAILABLE
CHANNEL 4
NOT AVAILABLE
CHANNEL 5
EX: 623 ± 12
EM: 676 ± 18.5
DYES: CY®5, Quasar®670, Liz®, Mustang Purple®
CHANNEL 6
NOT AVAILABLE
Other dyes/probes are possible depending on the system configuration.
The Azure Cielo Dx qPCR system for IVD use brings you the accuracy and sensitivity you need for your research, with intuitive touch screen software. With 6-target detection, the flexibility you need is at your fingertips.
USA: For IVD use. The Azure Cielo 3 Dx and Azure Cielo 6 Dx Real-time PCR systems are registered with the U.S. FDA as Class II 510(k) exempt devices.
Europe: For IVD use. The Azure Cielo 3 Dx and Azure Cielo 6 Dx systems are CE marked under the In Vitro Diagnostic Medical Devices Directive (98/79/EC). The CE IVD–authorized Azure Cielo Dx Systems are for distribution and use the following countries: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czechia, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Liechtenstein, Lithuania, Luxembourg, Malta, the Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, and the United Kingdom.
| Document Type | Description | |
|---|---|---|
| Brochure | Cielo Brochure | DOWNLOAD |
| Application Note | Using the Azure Cielo™ Real-Time PCR System for SARS-CoV-2 viral RNA detection | DOWNLOAD |
| Application Note | 6-Channel Multiplex qPCR Using Azure Cielo Real-Time PCR System | DOWNLOAD |
| Application Note | Sensitive and Accurate Single-Copy Amplicon Detection in Azure Cielo Real-Time PCR System | DOWNLOAD |
| Application Note | Azure Cielo™ Real-Time PCR Optical Design Eliminates the Need for Passive Reference Dye | DOWNLOAD |
| Application Note | What Sets the Cielo Apart | DOWNLOAD |
| Application Note | Detection of low copy telomeric genes with Cielo | DOWNLOAD |
| Application Note | GoTaq Enviro Wastewater SARS-CoV-2 | DOWNLOAD |
| Application Note | Azure Cielo 6 Compatibility with GenDx KMRtype and KMRtrack Workflows | DOWNLOAD |
| Technical Note | SNP Genotyping/Allelic Discrimination | DOWNLOAD |
| Technical Note | Adding Dyes and Color Compensation | DOWNLOAD |
| Application Note | Performance of a six-target multiplexing assay on the Cielo™ 6 using the Quantabio PerfeCTa® Multiplex qPCR ToughMix | DOWNLOAD |
| User Manual | Azure Cielo User Manual | DOWNLOAD |
