Azure Biosystems
Fast qPCR detection system for high-quality data with smart protocols, and robust durability
As a lab that has been carrying out high volume PCR testing for the last 12 months, the Azure Cielo Real-Time PCR machine has been a fantastic addition to our suite of equipment to facilitate this process. The comparison data we have achieved is exceptional along with EQA in established schemes. It is easy to use and has intuitive software and the support from the company has been exceptional. I wish it was on the market sooner in the pandemic.
-Sheryl Dunn, Nationwide Pathology
It is a very user friendly instrument. A very rapid thermocycler, with very good reproducibility. A good instrument to scale and increase produtivity. We have 3 in our lab and I really recomend this real time PCR system for diferent aplications.
-Natalia Salgueiro, Synlab
PCR system with perfect synchronization of results
-Shailendra Gurav, Goa College of Pharmacy Panaji
In my research field I use it to find the target copy of DNA samples. This PCR system gives me perfectly synchronized results that help me a lot. I recommend this products to others. It’s parts are also reasonable compared to those of others available products in market.
-Minhazul Islam, Bangladesh Agricultural University
Starting a run at the end of the day but need your data soon? Automatic email notifications from Cielo Manager have you covered.
Because the Cielo connects via Wi-Fi, this feature allows its software to not only notify you when your run is completed, but to also send the data straight to your inbox.
Each well is easily labeled with three identifiers:
The identifiers are used for data analysis and easy visualization of the amplification and standard curves.
Easily view all of your data at once or select specific wells/targets to view a subset of your data. Cielo’s software allows you to easily adjust threshold values by dragging the threshold line, or typing in the threshold value.
You can visualize amplification curves in linear or log view, and change the color of the amplification curves to easily distinguish the data by Sample ID or target.
Quickly review Cq values, and assess data uniformity and separation between dilutions.
The wells shown here are colored in a 2D Sample Heat Map based on Cq value, with the lowest Cq values in green and highest Cq values in red.
Well-based excitation and color-specific LED light sources ensure the excitation efficiency in every well for every channel is the same.
Coupled with scanning technology that collects multiple data points from each well, the Cielo’s well-based optical design eliminates the need for normalization dyes, such as ROX. Rather than a single, stationery light source and detector system, the Cielo utilizes 16-well simultaneous excitation and signal detection that ensures uniform data acquisition.
The Cielo is able to capture approximately 100,000 pixels/wells, enabling accurate, reproducible and sensitive representation of fluorescent intensities from each qPCR well.
Competitive systems are only able to acquire 1 pixel/well.
Single copy amplification was detected in 88 wells (92%) of the plate run on the Cielo, and in 77 wells (80%) on the plate run on the competitor system.
High-quality engineering includes Peltier-based thermal block system designed for superior thermal cycling and unmatched optical design to ensure every run is accurate and reproducible.
The Cielo is able to demonstrate reproducible quantitation down to a single copy of the target sequence.
Multiplex up to six targets on the Cielo, a true six-channel system. Flexibility is built into the system for the use of numerous commercially available dyes and applications.
Because ROX is not required, it can also be used as a probe.
| Parameter | Cielo 6 (AIQ060) | Cielo 3 (AIQ030) |
|---|---|---|
| Sample capacity (Wells) | 96 wells | 96 wells |
| Compatible Plates/Tubes | 96-well plates and tubes, 8- or 12-well strips and single tubes | 96-well plates and tubes, 8- or 12-well strips and single tubes |
| Reaction Volume | 1-50 uL (10-50 uL recommended) | 1-50 uL (10-50 uL recommended) |
| Dynamic Range | 10 Logs | 10 Logs |
| Excitation Source | LED Excitation range: 450-730 nm | LED Excitation range: 450-730 nm, 610-700 nm |
| Detection Channels | 6 | 3 |
| Multiplexing | Up to 6 targets | Up to 3 targets |
| Reaction Temperature | 4°C to 99.9°C | 4°C to 99.9°C |
| Max Block Ramp Rate | 6°C/sec | 6°C/sec |
| Average Sample Ramp Rate | 4°C/Sec | 4°C/Sec |
| Temperature Uniformity | ±0.2°C | ±0.2°C |
| Temperature Accuracy | ±0.1°C | ±0.1°C |
| Custom Dye/Chemistry | ✓ | ✓ |
| Onboard Memory | 32 GB Equivalent to 20,000 experiments | 32 GB Equivalent to 20,000 experiments |
| Unit dimensions (W x H x D) | 30.5 cm x 50.8 cm x 43.2 cm / 12” x 20” x 17" | 30.5 cm x 50.8 cm x 43.2 cm / 12” x 20” x 17" |
| Weight | 22.7kg / 50 lbs | 22.7kg / 50 lbs |
| Power requirements | 100-240 VAC, 8A; Consumption: 0.8kW (800 Watts) | 100-240 VAC, 8A; Consumption: 0.8kW (800 Watts) |
| Connectivity | USB, Wifi, Ethernet | USB, Wifi, Ethernet |
| Certifications | CE Certified, TUV Certified | CE Certified, TUV Certified |
| Dyes/Probes | Cielo 6 (AIQ060) | Cielo 3 (AIQ030) |
|---|---|---|
| • SYBR® Green • EvaGreen™ • FAM™ | ✓ | ✓ |
| • VIC® • HEX™ • JOE™ • CAL Fluor® 540 • CAL Fluor® Orange 560 | ✓ | ✓ |
| • TAMRA™ • Cy3 | ✓ | |
| • ROX™ • TEX®615, • CAL Fluor® Red 610 • CXR | ✓ | |
| • Cy®5 • Quasar®670, • Liz® • Mustang Purple® | ✓ | ✓ |
| • Cy®5.5 • Quasar 705 | ✓ |
Other dyes/probes are possible depending on the system configuration.
| Title | Link |
|---|---|
For Research Use Only
