The Ultimate Western Blot Imaging System

Azure 600

AZI600-01

Your Western Blot method shouldn’t be limited by your Western blot imager! With the Azure 600, it’s not.

Two scientists looking at multiplex fluorescent Western blot on Azure 600 Western blot imager

The Azure 600 gives you the flexibility you need for your research, while delivering solutions for quantitative Western Blot imaging. Azure Biosystems provides a unified Western Blot workflow, from the high-performance imaging system and analysis software to the reagents and consumables.

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Azure 600® | The All-around Application Ace

The All-around Application Ace

NIR
RGB
Chemi
Blue Light

White Light

Ultraviolet

Multiplex Western Blot Imager: the Best Option for Quantitation

Two-channel NIR Fluorescence Detection

The Azure 600 is the only system that offers two channel, laser-based IR and chemiluminescent detection, with the speed and sensitivity of film. It has the ability to image visible fluorescent dyes, standard EtBr and protein gels, and excitation for quantitative Western blot imaging in the NIR. This catchall Western blot imager improves your data quality imaging with infrared dyes and offers signal stability. Low background fluorescence imaging with NIR dyes allows you to study multiple proteins in a blot, even if those proteins overlap in molecular weight.

The Azure 600 offers laser technology with two IR detection channels enabling you to image more than one protein in an assay. It provides accurate and fast chemiluminescent detection, as well as the sensitivity, dynamic range, and linearity needed for quantitative blot analysis.

Two-fold Serial Dilution
Two-fold serial dilutions of AzureSpectra-800 labeled antibody were spotted on a nitrocellulose dot-blot, and imaged in the 800nm channel for 20 seconds
Fluorescent Western Blot of STAT1 and phospho-STAT1
Fluorescent Western Blot of STAT1 and phospho-STAT1. The blot was probed with anti-phospho-STAT1 and anti-STAT1 followed by fluorescent secondary antibodies, and then imaged on Azure Imaging system. Top right is the green channel, using IR-800; top left is the image of the red channel, using IR-700. Bottom image is both channels merged.

Three-channel RGB Fluorescent Detection

Fluorescent protein in native gel
Fluorescent protein in native gel imaged with an Azure imager
Zebrafish Expressing GFP
Zebrafish expressing GFP. Imaged with an Azure Imager.
Mouse with RFP-expressing subcutaneous tumor.
Whole mouse with RFP-expressing subcutaneous tumor. Imaged with an Azure Imager.
GFP- and mCherry-expressing E. coli.
Bacterial plate of GFP- and mCherry-expressing E. coli. Imaged using Azure Imager.

Our lab purchased Azure 600 to replace an old gel doc imager. Protein bands look sharp and the resolution is high. I also love the multiple image capturing can be repeated for multiple times with different duration. Besides, protein ladder (marker) can also be captured and this enables the comparison of protein bands with the protein ladder on the same blot. It is an ideal equipment for protein and molecular biology works.

Lai Yen
Fong Lai Yen
Kandiah Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman
Intuitive capture software controlled by a wide touchscreen

The Azure 600 provides high-resolution imaging that is ideal for publication purposes, as well as higher pixel well cap for higher dynamic range.

Use the preset protocols of the comprehensive list of typical detections, or generate your own custom protocols combining any excitation source, emission filter, image overlay, lens aperture, exposure time, focus, distance to the camera, and resolution.

High-resolution Imaging

The Azure 600 comes standard with a 9.1MP camera that provides high-resolution imaging perfect for publications. Change the sample to optics distance using adjustable height shelf for enhanced detection. Zoom into the area of interest with ROI imaging to reduce background.

Sensitivity Signal Surpasses Film

Revolutionize Your Chemiluminescent Workflows by Leaving the Darkroom

Quantitative Chemiluminescent Imaging
The same Western Blot was imaged on both x-ray film and the Azure Imaging System.
Figure 1. Saturation detection prevents errors in quantitation. The same Western Blot was imaged on both x-ray film and the Azure Imaging System. The Azure system detects when CCD saturation occurs and calculates an auto-exposure time to avoid saturated bands.

Get the best picture every time, and stop wasting money on film. Use Cy®5/Cy®3/Cy®2 Dyes, Blue-Excited DNA Dyes, and more. As the total solution for Western blot imaging and gel documentation, the Azure 600 is a multichannel, multimodal imager, with IR, visible light, and UV excitation channels. Finally, an imager that allows you to detect Cy dyes, Alexa® dyes, Safe dyes, and more!

The software notifies you when bands are saturated and are not suitable for quantitation.

The same Western Blot was imaged on both x-ray film and the Azure Imaging System.
Figure 1. Saturation detection prevents errors in quantitation. The same Western Blot was imaged on both x-ray film and the Azure Imaging System. The Azure system detects when CCD saturation occurs and calculates an auto-exposure time to avoid saturated bands.
Chemiluminescent imaging with color markers
Chemiluminescent Western Blots
Simultaneous imaging of chemiluminescent samples and colored molecular weight markers. Images are captured serially, and then overlaid within the capture software.​

The Azure 600 provides high-resolution imaging that is ideal for publication purposes, as well as higher pixel well cap for higher dynamic range.

You can overlay a color marker with the chemiluminescent signal for ease of documentation and molecular size determination.

 

Intuitive capture software controlled by a wide touchscreen
Artistic overlay of a scientist using an Azure 600 Western blot imaging system

Use the preset protocols of the comprehensive list of typical detections, or generate your own custom protocols combining any excitation source, emission filter, image overlay, lens aperture, exposure time, focus, distance to the camera, and resolution.

Compact Design
Footprint of the Azure 600® Imager (38 cm x 55 cm x 36 cm; W x H x D) vs. the iBright® FL-1000 (dotted line; 38 cm x 60 cm x 68 cm; W x H x D)
Footprint of the Azure 600® Imager (38 cm x 55 cm x 36 cm; W x H x D) vs. the iBright® FL-1000 (dotted line; 38 cm x 60 cm x 68 cm; W x H x D)

At 42 x 56 x 33 cm, the small size translates into more room on your bench

Chemiluminescent Western Blots
Simultaneous imaging of chemiluminescent samples and colored molecular weight markers. Images are captured serially, and then overlaid within the capture software.​
Artistic overlay of a scientist using an Azure 600 Western blot imaging system
Footprint of the Azure 600® Imager (38 cm x 55 cm x 36 cm; W x H x D) vs. the iBright® FL-1000 (dotted line; 38 cm x 60 cm x 68 cm; W x H x D)
Footprint of the Azure 600® Imager (38 cm x 55 cm x 36 cm; W x H x D) vs. the iBright® FL-1000 (dotted line; 38 cm x 60 cm x 68 cm; W x H x D)

Azure 600 User Spotlights

Connectivity through Wi-Fi, Ethernet, and USB with Microsoft Windows10 OS

Azure 600 Western blot Imaging system

The Azure 600 Western Blot Imager Offers:

Flexibility—Sensitivity and performance for fluorescence, chemiluminescence, trans-UV & visible, and true-color imaging with 4.8 OD dynamic range. High resolution CCD cameras with fast lens options, adjustable lens and illumination settings, trays with adjustable distance to the camera, and auto-detection and pre-calibrated focus.

Quantitative accuracy—Designed for generating publication quality images and easy quantitation. Our reagents, imaging system, and software work seamlessly together to help you follow best practices for Western Blot publication.

Intelligent workflow—Our user interface allows total customization over imaging protocols, while ensuring repeatability from sample to sample. Our systems feature Auto-Focus, Auto-Illumination and Filter Control, and Auto-Image Capture. An integrated touchscreen allows ease of use and it can easily be controlled by an external PC, if required.

Data integrity—Azure Biosystems meets the standards for publication in all major journals, and additionally offers software to enable 21 CFR Part 11 Compliance.

Works Well with Your Most-used Imaging Applications

Azure 600 Specifications

ParameterAzure 600
Detection9.1 MP camera
Pixel resolution16-bit, 65,536, Grayscale
Peltier Cooling-50°C regulated cooling
7 Position Filter Wheel
Trans UV302 and 365nm
Color Imaging
Trans-white imaging
Chemiluminescence
Laser-based NIR Imaging
Visible/RGB Fluorescent Imaging
NIR Fluorescence Imaging
Epi Blue Light Imaging
Total protein normalization channel
UV Safety Override Switch
Field of View20.5 x 16.5 cm
Unit dimensions (W x H x D)​42 x 56 x 33 cm / 16.5” x 22” x 13”
Weight​20 kg / 44 lbs
Power requirements100-240 VAC, 4A; Single Phase; Consumption: 0.4 kW (400 Watts)
SoftwareUnique touchscreen user interface for easy access to preset automatic applications
CertificationsCE, cTUVus

Literature

Document TypeDescription
BrochureAzure Imager BrochureDOWNLOAD
BrochureAzure Imaging Systems Model ConfigurationsDOWNLOAD
User ManualAzure Imaging Systems User ManualDOWNLOAD

Recent Publications

Compare Western Blot Imaging Systems

Choose the configuration that supports today’s studies, while maintaining the option to upgrade later on

Upgrades available for chemiluminescence, NIR fluorescence and RGB fluorescence.

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