Ultimate Near-infrared Fluorescent Imager

Azure 500

AZI500-01

The Azure 500 is a NIR fluorescent imager that’s the best solution for sensitive near-infrared and ECL Western blots, and gel documentation.

Azure 500 fluorescent imager
  • Multichannel, multimodal fluorescent imager, with IR, visible light, and UV excitation channels
  • Able to image and quantify two different targets in the same position more efficiently using near-infrared (NIR)
  • Normalize to fluorescent total protein stain or a housekeeping protein in the green channel without needing to strip and re-probe your Western blots

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Azure 500® | The Ultimate Near-infrared Imaging System

The Ultimate InfraRed Imaging System

NIR

Chemi

Blue Light

White Light

Ultraviolet
Upgradeable

Two-channel NIR Fluorescence Detection

Get out of the Darkroom!

The Azure 500’s laser technology offers two IR detection channels for the ability to image more than one protein in an assay. The Azure 500 provides accurate and fast chemiluminescent detection, as well as the sensitivity, dynamic range, and linearity needed for quantitative blot analysis. Get the best picture every time, and stop wasting money on film! UV, color imaging, blue-excited DNA dyes, Coomassie gel imaging, and more a total solution for Western blot imaging and gel documentation.

Two-fold Serial Dilution
Two-fold serial dilutions of AzureSpectra-800 labeled antibody were spotted on a nitrocellulose dot-blot, and imaged in the 800nm channel for 20 seconds

Two NIR fluorescence channels and lasers at 685nm and 784nm offer better sensitivity than LEDs or white light sources for Western blotting. The two-channel NIR Fluorescence detection enables sensitive multiplex Western Blotting with minimal background. Image and quantify two different targets in the same position more efficiently without needing to strip and re-probe your WBs.

Capture images of NIR fluorescence WB in multiplex, ECL WB, biomolecules tagged with fluorescent labels in gels, fluorescent and colorimetric stains in gels, Petri dishes, TLC, etc.

The Azure 500’s laser technology offers two IR detection channels for the ability to image more than one protein in an assay. The Azure 500 provides accurate and fast chemiluminescent detection, as well as the sensitivity, dynamic range, and linearity needed for quantitative blot analysis. Get the best picture every time, and stop wasting money on film! UV, color imaging, blue-excited DNA dyes, Coomassie gel imaging, and more a total solution for Western blot imaging and gel documentation.

Two NIR fluorescence channels and lasers at 685nm and 784nm offer better sensitivity than LEDs or white light sources for Western blotting. The two-channel NIR Fluorescence detection enables sensitive multiplex Western Blotting with minimal background. Image and quantify two different targets in the same position more efficiently without needing to strip and re-probe your WBs.

Capture images of NIR fluorescence WB in multiplex, ECL WB, biomolecules tagged with fluorescent labels in gels, fluorescent and colorimetric stains in gels, Petri dishes, TLC, etc.

Two-fold Serial Dilution
Two-fold serial dilutions of AzureSpectra-800 labeled antibody were spotted on a nitrocellulose dot-blot, and imaged in the 800nm channel for 20 seconds
Fluorescent Western Blot of STAT1 and phospho-STAT1
Fluorescent Western Blot of STAT1 and phospho-STAT1. The blot was probed with anti-phospho-STAT1 and anti-STAT1 followed by fluorescent secondary antibodies, and then imaged on Azure Imaging system. Top right is the green channel, using IR-800; top left is the image of the red channel, using IR-700. Bottom image is both channels merged.
Intuitive capture software controlled by a wide touchscreen
Two scientists looking at multiplex fluorescent Western blot on Azure 600 Western blot imager

The Azure 500 provides high-resolution imaging that is ideal for publication purposes, as well as higher pixel well cap for higher dynamic range.

Use the preset protocols of the comprehensive list of typical detections, or generate your own custom protocols combining any excitation source, emission filter, image overlay, lens aperture, exposure time, focus, distance to the camera, and resolution.

High-resolution Imaging

A 9.1MP camera provides high resolution imaging perfect for publications. Change the sample to optics distance using adjustable height shelf for enhanced detection. Zoom into the area of interest with ROI imaging to reduce background.

Two scientists looking at multiplex fluorescent Western blot on Azure 600 Western blot imager
Graphic showing how light intensity is affected by the distance of a sample to its light source
Azure Imagers are designed for capturing maximum light output from the sample. The CCD sensor is placed close to the sample in order to maximize light collection efficiency, which increases sensitivity. The Azure Imager camera is also directly above the sample. Having the camera as close to the sample as possible makes way for the best light collection efficiency.

Sensitivity Signal Surpasses Film

Fast and Sensitive Chemiluminescent Detection

Sensitive Detection of Proteins with Chemiluminescent Westerns
The same Western Blot was imaged on both x-ray film and the Azure Imaging System.
Figure 1. Saturation detection prevents errors in quantitation. The same Western Blot was imaged on both x-ray film and the Azure Imaging System. The Azure system detects when CCD saturation occurs and calculates an auto-exposure time to avoid saturated bands.

The software on the Azure 500 notifies you when bands are saturated and are not suitable for quantitation. Outperforms film performance while generates a quantitative, wide dynamic range image. Indicates eventual signal saturation for accurate quantitation.

The same Western Blot was imaged on both x-ray film and the Azure Imaging System.
Figure 1. Saturation detection prevents errors in quantitation. The same Western Blot was imaged on both x-ray film and the Azure Imaging System. The Azure system detects when CCD saturation occurs and calculates an auto-exposure time to avoid saturated bands.
Chemiluminescent Imaging with Color Markers
Chemiluminescent Western blot with color marker imaged with Azure Biosystems
Simultaneous imaging of chemiluminescent samples and colored molecular weight markers. Images are captured serially, and then overlaid within the capture software.​

The Azure 500 fluorescent imager provides high-resolution imaging that is ideal for publication purposes, as well as higher pixel well cap for higher dynamic range.

It gives you the option to overlay a color marker with the chemiluminescent signal for ease of documentation and molecular size determination.

 

Chemiluminescent Western blot with color marker imaged with Azure Biosystems
Simultaneous imaging of chemiluminescent samples and colored molecular weight markers. Images are captured serially, and then overlaid within the capture software.​

Upgradeable to Best Cater to Your Studies

Upgradeable to include three RGB fluorescence channels

The Azure 500 can be field upgraded to the Azure 600 for applications detecting fluorescent biomolecules in the visible range, Cy2/Cy3/Cy5, or similar fluorochromes.

*Optional Q Module (Green Channel)
Azure 500Q imager with green channel and 700nm and 800nm near-infrared lasers
Shown: Azure 500Q imager with green channel and 700nm and 800nm near-infrared lasers. You are able to add a Q Module for Total Protein Normalization of NIR Western blots using TotalStain Q. The upgrade Azure 500Q will also detect one more fluorescent target.

 

Add a Q Module for Total Protein Normalization of NIR Western blots using TotalStain Q, and detection of one more fluorescent target.

Azure 500Q imager with green channel and 700nm and 800nm near-infrared lasers
Shown: Azure 500Q imager with green channel and 700nm and 800nm near-infrared lasers. You are able to add a Q Module for Total Protein Normalization of NIR Western blots using TotalStain Q. The upgrade Azure 500Q will also detect one more fluorescent target.

Connectivity through Wi-Fi, Ethernet, and USB with Microsoft Windows10 OS

Azure 500 fluorescent imager

Azure 500 Fluorescent Western Blot Imager Offers:

Flexibility—Sensitivity and performance for fluorescence, chemiluminescence, trans-UV & visible, and true-color imaging with 4.8 OD dynamic range. High resolution CCD cameras with fast lens options, adjustable lens and illumination settings, trays with adjustable distance to the camera, and auto-detection and pre-calibrated focus.

Quantitative accuracy—Designed for generating publication quality images and easy quantitation. Our reagents, imaging system, and software work seamlessly together to help you follow best practices for Western Blot publication.

Intelligent workflow—Our user interface allows total customization over imaging protocols, while ensuring repeatability from sample to sample. Our systems feature Auto-Focus, Auto-Illumination and Filter Control, and Auto-Image Capture. An integrated touchscreen allows ease of use and it can easily be controlled by an external PC, if required.

Data integrity—The Azure 500 meets the standards for publication in all major journals, and additionally offers software to enable 21 CFR Part 11 Compliance.

Works Well with Your Most-used Imaging Applications

Azure 500 Specifications

ParameterAzure 500
Detection9.1 MP camera
Pixel resolution16-bit, 65,536, Grayscale
Peltier Cooling-50°C regulated cooling
7 Position Filter Wheel
Trans UV302 and 365nm
Color Imaging
Trans-white imaging
Chemiluminescence
Laser-based NIR Imaging
NIR Fluorescence Imaging
Epi Blue Light Imaging
Total protein normalization channelOptional
UV Safety Override Switch
Field of View20.5 x 16.5 cm
Unit dimensions (W x H x D)​42 x 56 x 33 cm / 16.5” x 22” x 13”
Weight​20 kg / 44 lbs
Power requirements100-240 VAC, 4A; Single Phase; Consumption: 0.4 kW (400 Watts)
SoftwareUnique touchscreen user interface for easy access to preset automatic applications
CertificationsCE, cTUVus

Literature

Document TypeDescription
BrochureAzure Imager BrochureDOWNLOAD
BrochureAzure Imaging Systems Model ConfigurationsDOWNLOAD
User ManualAzure Imaging Systems User ManualDOWNLOAD

Recent Publications

Compare Western Blot Imaging Systems

Choose the configuration that supports today’s studies, while maintaining the option to upgrade later on

Upgrades available for chemiluminescence, NIR fluorescence and RGB fluorescence.

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