Visible Fluorescent Imager

Azure 400

AZI400-01

The Azure 400 is a flexible fluorescent imager  that enables three-color fluorescent detection for dyes in the visible range.

Azure 400 Visible fluorescent imaging system
  • Capable of three-channel visible fluorescence detection
  • Enables sensitive multiplex detection of Western blots, fluorescent biomolecules and Cy2/Cy3/Cy5 or similar fluorochromes
  • Allows you to simultaneously image and quantify up to three different targets

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The Visible Fluorescence Virtuoso

Azure 400® | The Visible Fluorescence Virtuoso

RGB
Chemi
Blue Light

White Light

Ultraviolet
Upgradeable

Three-channel Visible Fluorescence Detection

UV, fluorescent color imaging, blue-excited DNA dyes, Coomassie gel imaging, and more

The Azure 400 offers 3-channel fluorescent detection for Western blots, chemiluminescent detection with the speed and sensitivity of film, and sensitive imaging for standard EtBr and protein gels. In addition to multiplex Westerns, the Azure 400 is a high-performance chemiluminescent imager and gel documentation workstation. This fluorescent imager provides accurate and fast chemiluminescent detection, as well as the sensitivity, dynamic range, and linearity needed for quantitative blot analysis.

Fluorescent protein in native gel
Fluorescent protein in native gel imaged with an Azure imager
GFP- and mCherry-expressing E. coli.
Bacterial plate of GFP- and mCherry-expressing E. coli. Imaged using Azure Imager.
GFP-expressing marijuana
GFP-expressing Marijuana dish. Imaged using Azure Imager.

Sensitivity Signal Surpasses Film

Chemiluminescent detection that surpasses X-ray film detection in sensitivity and dynamic range

Quantitative chemiluminescent imaging

Outperforms film performance while generates a quantitative, wide dynamic range image. Indicates eventual signal saturation for accurate quantitation.

The imaging chamber replaces the darkroom and film developers to generate a digital image including true color molecular weight marker. Obtain your images immediately and be ready to quantify bands in the analysis software.

The same Western Blot was imaged on both x-ray film and the Azure Imaging System.
Figure 1. Saturation detection prevents errors in quantitation. The same Western Blot was imaged on both x-ray film and the Azure Imaging System. The Azure system detects when CCD saturation occurs and calculates an auto-exposure time to avoid saturated bands.
Wide Dynamic Range

Capture images of visible fluorescence Western blot in multiplex, ECL Western blot, biomolecules tagged with fluorescent labels in gels, fluorescent and colorimetric stains in gels, Petri dishes, TLC, etc. True Color Imaging of translucent and opaque samples.

The same Western Blot was imaged on both x-ray film and the Azure Imaging System.
Figure 1. Saturation detection prevents errors in quantitation. The same Western Blot was imaged on both x-ray film and the Azure Imaging System. The Azure system detects when CCD saturation occurs and calculates an auto-exposure time to avoid saturated bands.
Artistic overlay of a scientist using an Azure 600 Western blot imaging system
Intuitive capture software controlled by a wide touchscreen

The Azure 400 is a fluorescent imager that provides high-resolution imaging is ideal for publication purposes, as well as higher pixel well cap for higher dynamic range.

Use the preset protocols of the comprehensive list of typical detections, or generate your own custom protocols combining any excitation source, emission filter, image overlay, lens aperture, exposure time, focus, distance to the camera, and resolution.

Artistic overlay of a scientist using an Azure 600 Western blot imaging system
Compact Design

At 42 x 56 x 33 cm, the small size translates into more room on your bench

Footprint of the Azure 600® Imager (38 cm x 55 cm x 36 cm; W x H x D) vs. the iBright® FL-1000 (dotted line; 38 cm x 60 cm x 68 cm; W x H x D)
Footprint of the Azure 600® Imager (38 cm x 55 cm x 36 cm; W x H x D) vs. the iBright® FL-1000 (dotted line; 38 cm x 60 cm x 68 cm; W x H x D)
Modular Fluorescent Imager to Support Your Research - Upgradeable to detect NIR700/NIR800

The Azure 400 can be field upgraded to the Azure 600 for applications detecting NIR700/NIR800 or similar fluorochromes.

Footprint of the Azure 600® Imager (38 cm x 55 cm x 36 cm; W x H x D) vs. the iBright® FL-1000 (dotted line; 38 cm x 60 cm x 68 cm; W x H x D)
Footprint of the Azure 600® Imager (38 cm x 55 cm x 36 cm; W x H x D) vs. the iBright® FL-1000 (dotted line; 38 cm x 60 cm x 68 cm; W x H x D)

Connectivity through Wi-Fi, Ethernet, and USB with Microsoft Windows10 OS

Azure 400 Visible fluorescent imaging system
The Azure 400 offers:


Flexibility—Sensitivity and performance for fluorescence, chemiluminescence, trans-UV & visible, and true-color imaging with 4.8 OD dynamic range. High resolution CCD cameras with fast lens options, adjustable lens and illumination settings, trays with adjustable distance to the camera, and auto-detection and pre-calibrated focus.

Quantitative accuracy—Designed for generating publication quality images and easy quantitation. Our reagents, imaging system, and software work seamlessly together to help you follow best practices for Western Blot publication.

Intelligent workflow—Our user interface allows total customization over imaging protocols, while ensuring repeatability from sample to sample. Our systems feature Auto-Focus, Auto-Illumination and Filter Control, and Auto-Image Capture. An integrated touchscreen allows ease of use and it can easily be controlled by an external PC, if required.

Data integrity—Azure Biosystems meets the standards for publication in all major journals, and additionally offers software to enable 21 CFR Part 11 Compliance.

Works Well with Your Favorite Applications

Research Spotlights from Users

Customer Spotlight

Advancing Bacterium Research with the Azure c400 at Virginia Tech

Customer Spotlight: Tam Nguyen, PhD Candidate at Virginia Tech Since the release of this interview, the Azure c400 has been upgraded to the Azure 400, a flexible fluorescent imager that enables three color fluorescent detection for dyes in the visible range. Microbiome research has grown exponentially in

Read More
Adarsha Devihalli in a fir field with a shovel collecting samples
Customer Spotlight

North Carolina’s Elite Christmas Tree Industry

Customer Spotlight: Adarsha Devihalli, PhD Candidate at North Carolina State Nestled in the southern region of the Appalachian Mountains is an environmentally beneficial abundance of Fraser fir—the most sought-after Christmas tree in the USA. Thanks to its charming aroma, soft and durable needles, and eye-catching silhouette the

Read More

Azure 400 Specifications

ParameterAzure 400
Detection9.1 MP camera
Pixel resolution16-bit, 65,536, Grayscale
Peltier Cooling-50°C regulated cooling
7 Position Filter Wheel
Trans UV302 and 365nm
Color Imaging
Trans-white imaging
Chemiluminescence
Visible/RGB Fluorescent Imaging
Epi Blue Light Imaging
Total protein normalization channel
UV Safety Override Switch
Field of View20.5 x 16.5 cm
Unit dimensions (W x H x D)​42 x 56 x 33 cm / 16.5” x 22” x 13”
Weight​20 kg / 44 lbs
Power requirements100-240 VAC, 4A; Single Phase; Consumption: 0.4 kW (400 Watts)
SoftwareUnique touchscreen user interface for easy access to preset automatic applications
CertificationsCE, cTUVus

Compare Western Blot Imaging Systems

Choose the configuration that supports today’s studies, while maintaining the option to upgrade later on

Upgrades available for chemiluminescence, NIR fluorescence and RGB fluorescence.

Literature

Document TypeDescription
BrochureAzure Imager BrochureDOWNLOAD
BrochureAzure Imaging Systems Model ConfigurationsDOWNLOAD
User ManualAzure Imaging Systems User ManualDOWNLOAD
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