Azure Biosystems
The Azure 400 is a flexible fluorescent imager that enables three-color fluorescent detection for dyes in the visible range.
The Azure 400 is capable of three-channel visible fluorescence detection, which enables sensitive multiplex detection of Western blots, fluorescent biomolecules and Cy2/Cy3/Cy5 or similar fluorochromes. This fluorescent imager allows users to simultaneously image and quantify up to three different targets.
White Light
UV, fluorescent color imaging, blue-excited DNA dyes, Coomassie gel imaging, and more
The Azure 400 offers 3-channel fluorescent detection for Western blots, chemiluminescent detection with the speed and sensitivity of film, and sensitive imaging for standard EtBr and protein gels. In addition to multiplex Westerns, the Azure 400 is a high-performance chemiluminescent imager and gel documentation workstation. This fluorescent imager provides accurate and fast chemiluminescent detection, as well as the sensitivity, dynamic range, and linearity needed for quantitative blot analysis.
Chemiluminescent detection that surpasses X-ray film detection in sensitivity and dynamic range
Outperforms film performance while generates a quantitative, wide dynamic range image. Indicates eventual signal saturation for accurate quantitation.
The imaging chamber replaces the darkroom and film developers to generate a digital image including true color molecular weight marker. Obtain your images immediately and be ready to quantify bands in the analysis software.
Capture images of visible fluorescence Western blot in multiplex, ECL Western blot, biomolecules tagged with fluorescent labels in gels, fluorescent and colorimetric stains in gels, Petri dishes, TLC, etc. True Color Imaging of translucent and opaque samples.
The Azure 400 is a fluorescent imager that provides high-resolution imaging is ideal for publication purposes, as well as higher pixel well cap for higher dynamic range.
Use the preset protocols of the comprehensive list of typical detections, or generate your own custom protocols combining any excitation source, emission filter, image overlay, lens aperture, exposure time, focus, distance to the camera, and resolution.
At 42 x 56 x 33 cm, the small size translates into more room on your bench
The Azure 400 can be field upgraded to the Azure 600 for applications detecting NIR700/NIR800 or similar fluorochromes.
• Flexibility—Sensitivity and performance for fluorescence, chemiluminescence, trans-UV & visible, and true-color imaging with 4.8 OD dynamic range. High resolution CCD cameras with fast lens options, adjustable lens and illumination settings, trays with adjustable distance to the camera, and auto-detection and pre-calibrated focus.
• Quantitative accuracy—Designed for generating publication quality images and easy quantitation. Our reagents, imaging system, and software work seamlessly together to help you follow best practices for Western Blot publication.
• Intelligent workflow—Our user interface allows total customization over imaging protocols, while ensuring repeatability from sample to sample. Our systems feature Auto-Focus, Auto-Illumination and Filter Control, and Auto-Image Capture. An integrated touchscreen allows ease of use and it can easily be controlled by an external PC, if required.
• Data integrity—Azure Biosystems meets the standards for publication in all major journals, and additionally offers software to enable 21 CFR Part 11 Compliance.
Customer Spotlight: Tam Nguyen, PhD Candidate at Virginia Tech Since the release of this interview, the Azure c400 has been upgraded to the Azure 400, a flexible fluorescent imager that enables three color fluorescent detection for dyes in the visible range. Microbiome research has grown exponentially in
| Parameter | Azure 400 |
|---|---|
| Detection | 9.1 MP camera |
| Pixel resolution | 16-bit, 65,536, Grayscale |
| Peltier Cooling | -50°C regulated cooling |
| 7 Position Filter Wheel | ✓ |
| Trans UV302 and 365nm | ✓ |
| Color Imaging | ✓ |
| Trans-white imaging | ✓ |
| Chemiluminescence | ✓ |
| Visible/RGB Fluorescent Imaging | ✓ |
| Epi Blue Light Imaging | ✓ |
| Total protein normalization channel | ✓ |
| UV Safety Override Switch | ✓ |
| Field of View | 20.5 x 16.5 cm |
| Unit dimensions (W x H x D) | 42 x 56 x 33 cm / 16.5” x 22” x 13” |
| Weight | 20 kg / 44 lbs |
| Power requirements | 100-240 VAC, 4A; Single Phase; Consumption: 0.4 kW (400 Watts) |
| Software | Unique touchscreen user interface for easy access to preset automatic applications |
| Certifications | CE, cTUVus |
Choose the configuration that supports today’s studies, while maintaining the option to upgrade later on
Upgrades available for chemiluminescence, NIR fluorescence and RGB fluorescence.
