Chemiluminescent Western Blot Imager

Chemiluminescent Western Blot Imager

Azure 300

AZI300-01

Come see the light! Say goodbye to the darkroom with a dependable, easy-to-use chemiluminescent imager.

Azure 300 chemiluminescent Western blot imager

The Azure 300 is a multichannel, multimodal imager, with visible fluorescence, visible light, and UV excitation channels. The system is fully upgradeable to a fluorescent Western Blot Imaging System.

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Azure 300® | The Darkroom Eliminator

The Darkroom Eliminator

Chemiluminescence

Blue Light

White Light

Ultraviolet
Upgradeable

Sensitive Chemiluminescent Imaging

chemiluminescent western blot signal
Chemiluminescent Western blotting- one signal, one protein.

Get the best picture every time, and stop wasting money on film! UV, color imaging, blue-excited DNA dyes, silver-stain, Coomassie gel imaging, and more. 

The Azure 300 offers the simplicity, speed and sensitivity of film detection, with better resolution and more quantitative results. This upgradeable chemiluminescent imager replaces a darkroom with film, while providing accurate and fast chemiluminescent detection, as well as the sensitivity, dynamic range, and linearity needed for quantitative blot analysis.

Quantitative Chemiluminescent Imaging
The same Western Blot was imaged on both x-ray film and the Azure Imaging System.
Figure 1. Saturation detection prevents errors in quantitation. The same Western Blot was imaged on both x-ray film and the Azure Imaging System. The Azure system detects when CCD saturation occurs and calculates an auto-exposure time to avoid saturated bands.

The Azure 300 offers digital chemiluminescent detection at a sensitivity equal to film,  without all the hassles. Simply place your Western blot into the system and acquire the image with the click of a button.

Outperforms film performance while generates a quantitative, wide dynamic range image. Indicates eventual signal saturation for accurate quantitation.

The imaging chamber replaces the darkroom and film developers to generate a digital image including true color molecular weight marker. Obtain your images immediately and be ready to quantify bands in the analysis software.

The same Western Blot was imaged on both x-ray film and the Azure Imaging System.
Figure 1. Saturation detection prevents errors in quantitation. The same Western Blot was imaged on both x-ray film and the Azure Imaging System. The Azure system detects when CCD saturation occurs and calculates an auto-exposure time to avoid saturated bands.
Compact Design
Footprint of the Azure Gel Imager vs. similar system (dotted line)
Footprint of Azure Gel Imager vs. similar system (dotted line)

At 42 x 56 x 33 cm, its small size translates into more room on your bench.

Intuitive capture software controlled by a wide touchscreen

The Azure 300 is a chemiluminescent imager that provides high-resolution imaging is ideal for publication purposes, as well as higher pixel well cap for higher dynamic range.

Use the preset protocols of the comprehensive list of typical detections, or generate your own custom protocols combining any excitation source, emission filter, image overlay, lens aperture, exposure time, focus, distance to the camera, and resolution.

Footprint of the Azure Gel Imager vs. similar system (dotted line)
Footprint of Azure Gel Imager vs. similar system (dotted line)
*Optional Q Module (Green Channel)
Azure 300Q imager with green channel for total protein normalization
Add visible and/or NIR fluorescent detection capabilities as your needs evolve with the Q Module. The Azure 300Q imager is equipped with a green channel for total protein normalization.

Add visible and/or NIR fluorescent detection capabilities as your needs evolve with the Q Module

Dynamic Range of 4.82 orders of magnitude

4.8 OD Dynamic Range

Field of View

20.5 x 16.5 cm

Azure 300Q imager with green channel for total protein normalization
Add visible and/or NIR fluorescent detection capabilities as your needs evolve with the Q Module. The Azure 300Q imager is equipped with a green channel for total protein normalization.

Research Spotlights from Users

Sarah Boyles teaching at St. Paul's School, where the Applied Science & Engineering Program uses an Azure 300 Imager
Customer Spotlight

Azure 300 Introduces Young Scientists to Western Blotting

Customer Spotlight: Sarah Boylan, Director of The Applied Science & Engineering Program at St. Paul’s School in New Hampshire At St. Paul’s School, a boarding school burrowed in New Hampshire, a robust and life-changing STEM program for students called The Applied Science & Engineering Program (ASEP) provides

Read More

Connectivity through Wi-Fi, Ethernet, and USB with Microsoft Windows10 OS

The Azure 300 is a wonderful gel imager and fits our needs perfectly. It’s compact and sleek – looks great on the bench. We just received it and are getting ready to take the first images soon. With the camera upgrade from the Azure 200 the images should be crisp and publication worthy. A solid and well-built instrument.

Karen Terry
Karen Terry
Department of Surgery, Duke University School of Medicine
Both the Azure 300 and Azure 300Q offer:

Flexibility—Sensitivity and performance for fluorescence, chemiluminescence, trans-UV & visible, and true-color imaging with 4.8 OD dynamic range. High resolution CCD cameras with fast lens options, adjustable lens and illumination settings, trays with adjustable distance to the camera, and auto-detection and pre-calibrated focus.

Quantitative accuracy—Designed for generating publication quality images and easy quantitation. Our reagents, imaging system, and software work seamlessly together to help you follow best practices for Western Blot publication.

Intelligent workflow—Our user interface allows total customization over imaging protocols, while ensuring repeatability from sample to sample. Our systems feature Auto-Focus, Auto-Illumination and Filter Control, and Auto-Image Capture. An integrated touchscreen allows ease of use and it can easily be controlled by an external PC, if required.

Data integrity—Azure Biosystems meets the standards for publication in all major journals, and additionally offers software to enable 21 CFR Part 11 Compliance.

Works Well with Your Favorite Applications

Azure 300 Specifications

ParameterAzure 300
Detection9.1 MP camera
Pixel resolution16-bit, 65,536, Grayscale
Peltier Cooling-50°C regulated cooling
7 Position Filter Wheel
Trans UV302 and 365nm
Color Imaging
Trans-white imaging
Chemiluminescence
Epi Blue Light Imaging
Total protein normalization channelOptional
UV Safety Override Switch
Field of View20.5 x 16.5 cm
Unit dimensions (W x H x D)​42 x 56 x 33 cm / 16.5” x 22” x 13”
Weight​17.7 kg / 39 lbs
Power requirements100-240 VAC, 4A; Single Phase; Consumption: 0.4 kW (400 Watts)
SoftwareUnique touchscreen user interface for easy access to preset automatic applications
CertificationsCE, cTUVus

Compare Western Blot Imaging Systems

Choose the configuration that supports today’s studies, while maintaining the option to upgrade later on

Upgrades available for chemiluminescence, NIR fluorescence and RGB fluorescence.

Literature

Document TypeDescription
BrochureAzure Imager BrochureDOWNLOAD
BrochureAzure Imaging Systems Model ConfigurationsDOWNLOAD
User ManualAzure Imaging Systems User ManualDOWNLOAD

Publications

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