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Western Blot Imaging Systems

Azure Imaging Systems

High-performance Western blot imaging systems capable of NIR fluorescence, visible fluorescence, and chemiluminescence.

Revolutionizing the Way You Western Blot!

Wonderful instrument! Terrific value and customer support!

Rated 5 out of 5

We’ve been very pleased with the Azure 600 Gel Imaging System. The quality and limit of detection for both the chemiluminescence and near-IR images is outstanding. Image acquisition is straightforward and user-friendly. The after-sales support is top notch – as there have been additional software upgrades and peripheral devices that were rolled-out post purchase. We’ve been happy with the new features. Our main contact at Azure (Vic Gagne) has been amazing. What sets this system apart from some of the bigger vendors is the ease-of-use and the personalized customer support. If your lab or core facility is looking for an imaging system, I highly recommend the Azure 600 Gel Imaging System.

-John Flanagan, Civetta Therapeutics

Azure 600 is easy to use and is great for all of your western blot needs

Rated 5 out of 5

The Azure 600 is easy to use and has been very helpful with my western blot analysis. The software is easy to use and I can get reproducible results. The ability to analyze for green or red wavelength of light is very helpful for being able to use different primary and secondary antibodies for my western blot analysis. I highly recommend Azure biosystems if you are in the market for a machine that can analyze your western blots for you and give you reproducible results.

-Austin Featherstone, Texas A&M University

Quick and easy imaging for many types of gels

Rated 5 out of 5

Bought a brand new C600 Imager and traded in our old C200 at the same time, and the process was super easy and smooth. The Technical Product Specialist I spoke with, Emma Paytas, was very knowledgeable and responded quickly to my emails, so I was able to make a well-informed and timely decision on which Imager would best fit our company’s needs. The installation and set-up was very simple, and would be intuitive for someone with no prior experience using the machine. They sent a hard-sided case and padding to trade our old imager in, so I didn’t have to worry about finding proper packaging to keep it from being damaged in transit. Our new Imager works great, with clear images of all our gels, and quick upload speed to our cloud.

-Barbara Frey, Primordial Genetics

Excellent sensitivity and feature set, fantastic for westerns and all other gels.

Rated 5 out of 5

Purchased this to replace our aging film developer. I’ve been a staunch user of film for over 25 years and until the Azure 600 showed up, I never found a digital system to match film’s sensitivity. With the Azure 600, my lab has stopped using film altogether as the sensitivity and ease of use beats film. Capture software is easy to use, and the analysis software is good (but could use some improvement). Fluorescent and ECL capture works great, and allows us to multiplex with different fluorophores when using fluorescent antibodies. Detection of silver stained proteins is also excellent, where you can see bands that are not visible with a naked eye. My lab tested 3 different imagers before settling on this one, and we do not regret our choice one bit. Overall, a fantastic and powerful imager that now is used daily by my lab and others at my institution after the word of it has spread.

-Peter Pelka, University of Manitoba

Experts Trust Azure Imagers

Reasons to Choose an Azure Imager

laser-based imaging

Lasers for Sensitive NIR imaging

Using lasers for NIR fluorescent imaging sets Azure Imagers apart from competitors. They are the only ones on the market to use lasers.

Narrow, specific laser excitation leads to higher signal, with less background and increases the overall sensitivity of your Western blots.

flexibility

Total Application Flexibility through Upgrades

Azure Imagers provide the sensitivity, adaptability, and performance that are needed by a wide variety of experimental processes.

Use the same imager to keep up with research changes and introduce new techniques as your scope continues to develop.

  • Ideal for fluorescent, chemiluminescent, ultraviolet, and visible imaging with a 4.8 OD range
  • Equipped with high-resolution CCD cameras with fast lens options, adjustable optical and lens settings, adjustable height tray with auto-detection, and pre-calibrated focus
  • Simplified imager designs and fewer mechanics to help minimize the need for frequent recalibrations and help mitigate the risk of damage through everyday use
  • Designed to be compatible with the widest variety of light sources to help ensure greatest dye compatibility and highest intensity for optimal specificity
workflow

Intelligent Workflow for Easy Customization

From upstream conjugation to downstream analysis, Azure helps provide the most inclusive workflow for your research.

A full HD 13.3″ touchscreen allows you to totally customize your imaging protocols, while ensuring repeatability from sample to sample.

  • Features auto-focus, auto-light control, auto-image capture, and dynamic flat fielding
  • Integrated touchscreen for ease of use and easy configuration with an external PC
  • Experimental data files that can be quickly exported to open-source file types
data integrity

Best Practices for Publishable Results

Designed with your future publications in mind. Azure reagents, imaging systems, and software work together seamlessly to help you accurately follow Western blot best practices and generate journal-ready images.

  • Exports publication-ready .tiff files for immediate, high-quality results

  • High-resolution CCD camera with the highest quantum efficiency for the most accurate representation of chemiluminescent reactions
data integrity

Support for GxP

Designed for compliance with U.S. FDA 21 CFR Part 11 guidelines*.

  • Provides audit reports and enables user roles for GMP/GLP labs
  • IQOQ* for both image and analysis software

*21 CFR Part 11, and IQOQ are optional modules.

The Western Blot Imaging System of Your Dreams

Direct, Mirror-free Detection

Azure Imagers deliver better performance through direct,-mirror-free detection. The sensor is placed close to the sample to maximize light collection efficiency and sensitivity.

The camera is strategically placed directly above the sample. Having the camera as close to the sample as possible makes way for the best light collection efficiency.

Graphic showing how light intensity is affected by the distance of a sample to its light source
Azure Imagers are designed for capturing maximum light output from the sample. The CCD sensor is placed close to the sample in order to maximize light collection efficiency, which also increases sensitivity.

Fluorescent Channel for Accurate Quantification

Solve the issue of housekeeping proteins, which lack linearity and normalization accuracy at high protein loads. Several Azure Imaging Systems can be upgraded with the Azure Q Module, an optional green fluorescence channel intended for detecting Total Protein Stains, such as TotalStainQ.

This can be used for efficient total protein normalization (TPN) of quantitative Western blots and helps achieve the most accurate quantification.

Quantitative Chemiluminescence

Just as sensitive as film, but easier and quantitative, the Azure Imaging Systems will revolutionize your chemiluminescent workflows and eliminate your need for a darkroom.

All of our models provide high-resolution imaging that’s perfect for publications. Change the sample to optics distance using adjustable height shelf for enhanced detection. Zoom into the area of interest with ROI imaging to reduce background.

Complete flexibility to control imaging, exposure time, and detection of color markers. The acquisition modes include preview, automatic and manual image capture, multiple and cumulative exposures

The same Western Blot was imaged on both x-ray film and the Azure Imaging System.
Saturation detection prevents errors in quantitation. The same Western Blot was imaged on both x-ray film and the Azure Imaging System. The Azure system detects when CCD saturation occurs and calculates an auto-exposure time to avoid saturated bands.

Visible Fluorescence

With high resolution, high sensitivity, low background fluorescence imaging, and up to 4 channel fluorescent detection from RGB to NIR spectrum, the Azure Imaging Systems enable quantitative Western Blotting and a whole lot more.

Full-color multiplex RGB LEDs and filters broaden your imaging capabilities to visible fluorescence wavelengths, increasing flexibility and expanding multiplexing options while keeping system-size compact and value high.

Choose the Azure 400 for visible fluorescence, the Azure 500 for NIR fluorescence, or the Azure 600 for both visible and NIR fluorescence.

Digital image of 3-color fluorescent multiplexed Western blot
Digital image of 3-color Western Blot using Azure Biosystems 600 imager. Lanes (from left to right) loaded with 1, 2, 5, 10, 20 μg HeLa cell lysate. Probed for tubulin (top), beta actin (middle) and GAPDH (bottom). The following settings were used: Light sources 6/7/4; Exposure time 1s/13s 204ms/677ms; Filter positions 6/7/4; Aperture 6400; Focus 5000/5250/5000; bin level 1x1.

Diverse Imaging Applications

Ready to expand your research past gels and membranes? What truly sets Azure Imaging Systems apart is their ability to image more than just blots, using cameras ranging from 5.4MP to 9.1MP.

From tissue sections and media plates to in vivo fluorescence of three-dimensional samples, the Azure Imaging Systems have unmatched depth-of-field that easily enables unparalleled imaging flexibility. Azure Imagers enable you to image 3D samples, such as mice and zebrafish.

Shown from left to right: Fluorescent protein in native gel, Mouse with RFP-expressing subcutaneous tumor, mouse brain tissue slides, three-color fluorescent Western blot, mice imaged RGB automatic mode, mouse brain slides, GFP/m-Cherry dish, GFP-expressing Arabidopsis thaliana

Snapshot of Compatible Dyes

Multichannel, multimodal imagers, with near-infrared, visible light, and UV excitation channels.

Detect Cy dyes, Alexa dyes, Safe dyes, UV-activated stain for protein gels (tri-halo compound stain), and more*

*Other dyes are also possible depending on your Azure Imager configuration.

  • Alexa Fluor 488
  • Alexa Fluor 546
  • Alexa Fluor 555
  • Alexa Fluor 633
  • Alexa Fluor 647
  • Alexa Fluor 680
  • Azure TotalStain
  • AzureRed
  • AzureSpectra 488
  • AzureSpectra 550
  • AzureSpectra 650
  • AzureSpectra 700
  • AzureSpectra 800
  • Chemiluminescence
  • Coomassie Blue
  • Coomassie Fluor™
  • Crystal Violet
  • Cy®2
  • Cy®3
  • Cy®5
  • Deep Purple™
  • DyLight 488
  • DyLight 550
  • DyLight 633
  • DyLight 650
  • DyLight 680
  • DyLight 755
  • DyLight 800
  • ECL Plex™
  • Ethidium Bromide
  • GelStar®
  • IRDye 650
  • IRDye 680LT
  • IRDye 680RD
  • IRDye 700DX
  • IRDye 750
  • IRDye 800CW
  • IRDye 800RS
  • Orange
  • Ponceau
  • Qdot 525
  • Qdot 565
  • Qdot 585
  • Qdot 605
  • Qdot 655
  • Qdot 705
  • Qdot 755
  • Revert™
  • Silver Stain
  • SYBR Gold
  • SYBR Safe
  • SYBR® Green
  • SYPRO® Orange
  • SYPRO Red
  • SYPRO Ruby
  • SYPRO Tangerine
  • Alexa Fluor 488
  • Alexa Fluor 546
  • Alexa Fluor 555
  • Alexa Fluor 633
  • Alexa Fluor 647
  • Alexa Fluor 680
  • Azure TotalStain
  • AzureRed
  • AzureSpectra 488
  • AzureSpectra 550
  • AzureSpectra 650
  • AzureSpectra 700
  • AzureSpectra 800
  • Chemiluminescence
  • Coomassie Blue
  • Coomassie Fluor™
  • Crystal Violet
  • Cy®2
  • Cy®3
  • Cy®5
  • Deep Purple™
  • DyLight 488
  • DyLight 550
  • DyLight 633
  • DyLight 650
  • DyLight 680
  • DyLight 755
  • DyLight 800
  • ECL Plex™
  • Ethidium Bromide
  • GelStar®
  • IRDye 650
  • IRDye 680LT
  • IRDye 680RD
  • IRDye 700DX
  • IRDye 750
  • IRDye 800CW
  • IRDye 800RS
  • Orange
  • Ponceau
  • Qdot 525
  • Qdot 565
  • Qdot 585
  • Qdot 605
  • Qdot 655
  • Qdot 705
  • Qdot 755
  • Revert™
  • Silver Stain
  • SYBR Gold
  • SYBR Safe
  • SYBR® Green
  • SYPRO® Orange
  • SYPRO Red
  • SYPRO Ruby
  • SYPRO Tangerine

Compare Western Blot Imaging Systems

Choose the configuration that supports today’s studies, while maintaining the option to upgrade later on

Azure Imager Specifications

Azure Imager Specifications
ParameterAzure 600Azure 500Azure 400Azure 300Azure 280Azure 200
Detection9.1 MP camera9.1 MP camera9.1 MP camera9.1 MP camera6.1 MP camera5.4 MP camera
Pixel resolution16-bit, 65,536, Grayscale16-bit, 65,536, Grayscale16-bit, 65,536, Grayscale16-bit, 65,536, Grayscale16-bit, 65,536, Grayscale16-bit, 65,536, Grayscale
Peltier Cooling-50°C regulated cooling-50°C regulated cooling-50°C regulated cooling-50°C regulated cooling-50°C regulated coolingN/A
Extended Dynamic RangeUp to 24-bit image acquisitionUp to 24-bit image acquisitionUp to 24-bit image acquisitionUp to 24-bit image acquisitionUp to 24-bit image acquisition
7 Position Filter Wheel✓✓✓✓✓✓
Trans UV302 and 365nm✓✓✓✓✓✓
Color Imaging✓✓✓✓✓✓
Epi-white Imaging✓✓✓✓✓✓
Trans-white imaging✓✓✓✓✓
Chemiluminescence✓✓✓✓✓
Laser-based NIR Imaging✓✓
Visible/RGB Fluorescent Imaging✓✓
Epi Blue Light Imaging✓✓✓✓✓✓
Total protein normalization channel✓Optional✓Optional
UV Safety Override Switch✓✓✓✓✓✓
Field of View*21 x 16.5 cm21 x 16.5 cm21 x 16.5 cm21 x 16.5 cm20.4 x 16.6 cm20 x 15 cm
Lens optionsPlease contact us to explore lens options for Azure imagers
Unit dimensions (W x H x D)​42 x 56 x 33 cm / 16.5” x 22” x 13”42 x 56 x 33 cm / 16.5” x 22” x 13”42 x 56 x 33 cm / 16.5” x 22” x 13”42 x 56 x 33 cm / 16.5” x 22” x 13”42 x 56 x 33 cm / 16.5” x 22” x 13”42 x 56 x 33 cm / 16.5” x 22” x 13”
Weight​20 kg / 44 lbs20 kg / 44 lbs20 kg / 44 lbs20 kg / 44 lbs20 kg / 44 lbs17.7 kg / 39 lbs
Power requirements100-240 VAC, 4A; Single Phase; Consumption: 0.4 kW (400 Watts)100-240 VAC, 4A; Single Phase; Consumption: 0.4 kW (400 Watts)100-240 VAC, 4A; Single Phase; Consumption: 0.4 kW (400 Watts)100-240 VAC, 4A; Single Phase; Consumption: 0.4 kW (400 Watts)100-240 VAC, 4A; Single Phase; Consumption: 0.4 kW (400 Watts)100-240 VAC, 4A; Single Phase; Consumption: 0.4 kW (400 Watts)
ConnectionUnique touchscreen user interface for easy access to preset automatic applicationsUnique touchscreen user interface for easy access to preset automatic applicationsUnique touchscreen user interface for easy access to preset automatic applicationsUnique touchscreen user interface for easy access to preset automatic applicationsUnique touchscreen user interface for easy access to preset automatic applicationsUnique touchscreen user interface for easy access to preset automatic applications
CertificationsCE Compliance CertifiedCE Compliance CertifiedCE Compliance CertifiedCE Compliance CertifiedCE Compliance CertifiedCE Compliance Certified

*Enhanced Field of View up to 25.5×21 cm available

Azure Imaging Systems are used for Western blot imaging and gel documentation, including NIR fluorescence, visible fluorescence, chemiluminescence, ultraviolet, and visible imaging applications. They support a wide range of workflows, from quantitative Western blots to imaging gels, membranes, tissue sections, media plates, and 3D samples.

Azure Imaging Systems stand out because they use laser-based NIR fluorescence imaging for increased signal, lower background, and greater Western blot sensitivity. Systems also use direct, mirror-free detection, placing the sensor close to the sample to maximize light collection efficiency and improve sensitivity.

Yes, Azure Imaging Systems support chemiluminescent and fluorescent Western blot imaging, with configurations available for visible fluorescence, NIR fluorescence, or both. For example, systems such as the Azure 600 support visible fluorescence, NIR fluorescence, and chemiluminescence. Other models in the Azure Imaging Systems line can be selected based on the lab’s current detection needs and future upgrade plans.

Azure Imaging Systems support quantitative Western blotting through high-resolution CCD cameras, wide dynamic range imaging, saturation detection, fluorescence-based normalization options, and software designed to generate publication-ready images. These features help researchers reduce quantitation errors and produce more reliable data.

Azure Imaging Systems are well suited for chemiluminescent Western blotting, visible fluorescence, NIR fluorescence, gel imaging, total protein normalization, tissue section imaging, and media plate imaging. Their upgradeable configurations allow labs to adapt the system as research needs evolve.

Frequently Asked Questions

Applications

Azure Imaging Systems support multiple applications and are able to record data from fluorescent and chemiluminescent Western blots, bacterial plates, protein gels, nucleic acid gels, and more.

Recently Published Research

Azure Imagers have been published in over 10,000 publications worldwide. Our high-resolution CCD cameras are the reason behind reproducible data and publication-ready images.

For Research Use Only

Ready to visualize your data with unmatched accuracy trusted by thousands of users? Get pricing on an Azure Imager today.


Headquarters:
Azure Biosystems, Inc.
6747 Sierra Court, Suite A-B
Dublin, CA 94568
United States

Tel: (925) 307 7127
Fax: (925) 905-1816
Info@azurebiosystems.com

Azure Biosystems Pte. Ltd.
68 Circular Road, #02-01
Singapore, 049422

Copyright © 2026 Azure Biosystems Inc.
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