Western Blot Imaging Systems

Azure Imaging Systems

Revolutionizing the way you Western blot! Azure Imagers are high performance Western blot imaging systems capable of NIR fluorescence, visible fluorescence, and chemiluminescence.

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What Scientists are Saying about Azure Imagers

Wonderful instrument! Terrific value and customer support!

5/5

We’ve been very pleased with the Azure 600 Gel Imaging System. The quality and limit of detection for both the… more

Its sensitivity for western blots is amazing.

5/5

In our lab, we have Azure c300 imager purchased years ago and it has never failed our expectations… more

Experts Trust Azure Imagers

Reasons to Choose an Azure Imager

laser-based imaging

Lasers for Sensitive NIR imaging

Using lasers for NIR fluorescent imaging sets the Azure Imagers apart from competitors. Our Imagers are the only ones on the market to use lasers. Narrow, specific laser excitation leads to higher signal, with less background and increases the overall sensitivity of your Western blots.

flexibility

Total Application Flexibility through Upgrades

Azure Imagers provide the sensitivity, adaptability, and performance that are needed by a wide variety of experimental processes—allowing you to use the same imager to keep up with research changes and introduce new techniques as your scope continues to develop. Find out which Azure Imager is best for your research by reviewing the comparison table below.

  • Ideal for fluorescent, chemiluminescent, ultraviolet, and visible imaging with a 4.8 OD range
  • Equipped with high-resolution CCD cameras with fast lens options, adjustable optical and lens settings, adjustable height tray with auto-detection, and pre-calibrated focus
  • Simplified imager designs and fewer mechanics to help minimize the need for frequent recalibrations and help mitigate the risk of damage through everyday use
  • Designed to be compatible with the widest variety of light sources to help ensure greatest dye compatibility and highest intensity for optimal specificity
workflow

Intelligent Workflow for Easy Customization

From upstream conjugation to downstream analysis, we help provide the most inclusive workflow for your research. The full HD 13.3" touchscreen user interface allows for total customization over imaging protocols, while ensuring repeatability from sample to sample.

  • Features auto-focus, auto-light control, auto-image capture, and dynamic flat fielding
  • Integrated touchscreen for ease of use and easy configuration with an external PC
  • Experimental data files that can be quickly exported to open-source file types
data integrity

Best Practices for Publishable Results

Every piece of technology from Azure Biosystems is designed with your future publications in mind. Our reagents, imaging systems, and software work together seamlessly to help you accurately follow Western blot best practices and generate journal-ready images.

  • Systems and products meet the standards for publications in all major journals, and software can enable 21 CFR Part 11 Compliance
  • Capable of imaging blots up to 420 DPI for high-quality, publication-ready results
  • High-resolution CCD camera with the highest quantum efficiency for the most accurate representation of a chemiluminescent reaction

The Western Blot Imaging System of Your Dreams

Fluorescent Channel for Accurate Quantification

Solve the issue of housekeeping proteins, which lack linearity and normalization accuracy at high protein loads. Several Azure Imaging Systems can be upgraded with the Azure Q Module, an optional green fluorescence channel intended for detecting Total Protein Stains, such as TotalStainQ.

This can be used for efficient total protein normalization (TPN) of quantitative Western blots and helps achieve the most accurate quantification.

Quantitative Chemiluminescence

Just as sensitive as film, but easier and quantitative, our Azure Imaging Systems will revolutionize your chemiluminescent workflows and eliminate your need for a darkroom.

All of our models provide high-resolution imaging that’s perfect for publications. Change the sample to optics distance using adjustable height shelf for enhanced detection. Zoom into the area of interest with ROI imaging to reduce background.

Complete flexibility to control imaging, exposure time, and detection of color markers. The acquisition modes include preview, automatic and manual image capture, multiple and cumulative exposures.

The same Western Blot was imaged on both x-ray film and the Azure Imaging System.
Saturation detection prevents errors in quantitation. The same Western Blot was imaged on both x-ray film and the Azure Imaging System. The Azure system detects when CCD saturation occurs and calculates an auto-exposure time to avoid saturated bands.

Visible Fluorescence

With high resolution, high sensitivity, low background fluorescence imaging and up to 4 channel fluorescent detection from RGB to NIR spectrum, the Azure Imaging System enables quantitative Western Blotting and a whole lot more.

Full-color multiplex RGB LEDs and filters broaden your imaging capabilities to visible fluorescence wavelengths, increasing flexibility and expanding multiplexing options while keeping system-size compact and value high.

Choose the Azure 400 for visible fluorescence, the Azure 500 for NIR fluorescence, or the Azure 600 for both visible and NIR fluorescence.

Digital image of 3-color fluorescent multiplexed Western blot
Digital image of 3-color Western Blot using Azure Biosystems 600 imager. Lanes (from left to right) loaded with 1, 2, 5, 10, 20 μg HeLa cell lysate. Probed for tubulin (top), beta actin (middle) and GAPDH (bottom). The following settings were used: Light sources 6/7/4; Exposure time 1s/13s 204ms/677ms; Filter positions 6/7/4; Aperture 6400; Focus 5000/5250/5000; bin level 1x1.

Diverse Imaging Applications

Ready to expand your research past gels and membranes? What truly sets Azure Imaging Systems apart is their ability to image more than just blots with cameras ranging from 5.4MP to 9.1MP.

From tissue sections and media plates to in vivo fluorescence of three-dimensional samples, the Azure Imaging Systems have unmatched depth-of-field that easily enables unparalleled imaging flexibility. Azure Imagers enable you to image three-dimensional samples, such as mice and zebrafish.

Shown from left to right: Fluorescent protein in native gel, Mouse with RFP-expressing subcutaneous tumor, mouse brain tissue slides, three-color fluorescent Western blot, mice imaged RGB automatic mode, mouse brain slides, GFP/m-Cherry dish, GFP-expressing Arabidopsis thaliana

Snapshot of Compatible Dyes

Multichannel, multimodal imagers, with near-infrared, visible light, and UV excitation channels.

Detect Cy dyes, Alexa dyes, Safe dyes, UV-activated stain for protein gels (tri-halo compound stain), and more*

*Other dyes are also possible depending in your Azure Imager configuration.

Compare Western Blot Imaging Systems

Choose the configuration that supports today’s studies, while maintaining the option to upgrade later on

Upgrades available for chemiluminescence, NIR fluorescence and RGB fluorescence.

Azure Imager Specifications

ParameterAzure 600Azure 500Azure 400Azure 300Azure 280Azure 200
Detection9.1 MP camera6.1 MP camera5.4 MP camera
Pixel resolution16-bit, 65,536, Grayscale
Peltier Cooling-50°C regulated coolingN/A
7 Position Filter Wheel
Trans UV302 and 365nm
Color Imaging
Trans-white imaging
Chemiluminescence
Laser-based NIR Imaging
Visible/RGB Fluorescent Imaging
Epi Blue Light Imaging
Total protein normalization channelOptionalOptional
UV Safety Override Switch
Field of View20.5 x 16.5 cm20.4 x 16.6 cm20 x 15 cm
Unit dimensions (W x H x D)​42 x 56 x 33 cm / 16.5” x 22” x 13”
Weight​20 kg / 44 lbs17.7 kg / 39 lbs
Power requirements100-240 VAC, 4A; Single Phase; Consumption: 0.4 kW (400 Watts)
ConnectionUnique touchscreen user interface for easy access to preset automatic applications
CertificationsCE Compliance Certified

Applications

Azure Imaging Systems support multiple applications and are able to record data from fluorescent and chemiluminescent Western blots, bacterial plates, protein gels, nucleic acid gels, and more.

Recently Published

Azure Imagers have been published in over 2,000 publications worldwide. Our high-resolution CCD cameras are the reason behind reproducible data and publication-ready images.

Resources

Document TypeDescription
BrochureAzure Imaging Systems BrochureDOWNLOAD
BrochureAzure Imaging Systems Model ConfigurationsDOWNLOAD
User ManualAzure Imaging Systems User ManualDOWNLOAD
App NoteBrowse All Application NotesBROWSE

Get ready to visualize your data with unmatched accuracy trusted by thousands of scientists. Get pricing on an Azure Imager today.

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