TotalStain Q (PVDF)

$167.25 (USD)

Accurate and quantitative fluorescent total protein stain to perform normalization of Western blots imaged on both camera-based and laser-based instruments with green channel or Safe-Dyes channel. Compatible with subsequent Western Blot detection using chemiluminescence or Near Infrared (NIR) fluorescence.

The linear range between 1-50 μg of lysate makes it ideal for normalization of low-expressed proteins that require high loads of protein to reach the sensitivity for the detection of the target. TotalStain Q solves the issue of housekeeping proteins that lack linearity and normalization accuracy at high protein loads.

  • Simple 20-minute dye-and-wash protocol
  • Wide linear dynamic range across lysates of various tissue types
  • Compatible with both chemiluminescent and NIR fluorescence

Sufficient amount for 15 mini-blots

Catalog number: AC2225

Description

[tabs style=”default”][tab title=”Product Description”]

Fluorescent Total Protein Stain for Blots on PVDF membranes

Accurate and quantitative fluorescent total protein stain to perform normalization of Western Blots. Blots can be imaged on both camera-based and scanner instruments with green channel or Safe-Dyes channel.

TotalStain Q only adds a short 20-minute step to your Western Blot protocol, between protein transfer and blocking buffer incubation.

TotalStain Q is compatible with subsequent Western Blot detection using chemiluminescence or Near Infrared (NIR) fluorescence.

The linear range between 1-50 μg of lysate makes it ideal for normalization of low-expressed proteins that require high loads of protein to reach the sensitivity for the detection of the target. TotalStain Q solves the issue of housekeeping proteins that lack linearity and normalization accuracy at high protein loads.

Features

• Simple 20-minute dye-and-wash protocol
• Wide linear dynamic range across lysates of various tissue types
• Compatible with both chemiluminescent and NIR fluorescence

[/tab][tab title=”Product Literature”]

Download PDFAzure Total Protein Stains Brochure

[/tab][tab title=”Protocols & Product Insert”]

Protocols & Product Inserts

Download PDFTotalStain Q – PVDF Total Protein Stain Kit
[/tab][tab title=”Safety Data Sheets”]

Safety Data Sheets

Download PDFMSDS-SDS_TotalStain Q-PVDF Stain Solution

Download PDFMSDS-SDS_TotalStain Q-Wash Solution

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Documents

Document TypeDescription
BrochureAzure Total Protein Stains BrochureDOWNLOAD
BrochureTotal Protein Stains BrochureDOWNLOAD
Safety Data SheetTotalStain Q - PVDF Stain Solution SDSDOWNLOAD
Safety Data SheetTotalStain Q Wash Solution SDSDOWNLOAD
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