TotalStain Q (NC)

SKU: AC2227

$192.34 (USD)

Fluorescent Total Protein Stain for Western blots on nitrocellulose membranes

Accurate and quantitative fluorescent total protein stain to perform normalization of Western blots. Blots can be imaged on both camera-based and scanner instruments with green channel or Safe-Dyes channel.

TotalStain Q only adds a short 24-minute step to your Western blot protocol, between protein transfer and blocking buffer incubation. It is compatible with subsequent Western blot detection using chemiluminescence or Near Infrared (NIR) fluorescence.

Kit is good for 15 membranes

 

features

TotalStain Q is compatible with subsequent Western blot detection using chemiluminescence or Near Infrared (NIR) fluorescence.

The linear range between 1-50 μg of lysate makes it ideal for normalization of low-expressed proteins that require high loads of protein to reach the sensitivity for the detection of the target. TotalStain Q solves the issue of housekeeping proteins that lack linearity and normalization accuracy at high protein loads.

  • Simple 24-minute dye-and-wash protocol
  • Wide linear dynamic range across lysates of various tissue types
  • Compatible with both chemiluminescent and NIR fluorescence
HeLa-lysate-5μg-replicates-imaged-using-TotalStain-Q
HeLa lysate 10μg replicates imaged using TotalStain Q (Cy3 channel, green) and pSTAT3 on an Azure Sapphire Imager.

Documents

Document TypeDescription
BrochureAzure Total Protein Stains BrochureDOWNLOAD
BrochureTotalStain Q – NC Total Protein Stain KitDOWNLOAD
Safety Data SheetTotalStain Q - NC Stain Solution SDSDOWNLOAD
Safety Data SheetTotalStain Q - Wash Solution SDSDOWNLOAD
ProtocolTotalStain Q NC Short ProtocolDOWNLOAD

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