TotalStain Q (NC)

SKU: AC2227

$201.96 (USD)

Fluorescent Total Protein Stain for Western blots on nitrocellulose membranes

Accurate and quantitative fluorescent total protein stain to perform normalization of Western blots. Blots can be imaged on both camera-based and scanner instruments with green channel or Safe-Dyes channel.

TotalStain Q only adds a short 24-minute step to your Western blot protocol, between protein transfer and blocking buffer incubation. It is compatible with subsequent Western blot detection using chemiluminescence or Near Infrared (NIR) fluorescence.

  • Kit contains enough material for 15 membranes

View associated documents >

features

TotalStain Q is compatible with subsequent Western blot detection using chemiluminescence or Near Infrared (NIR) fluorescence.

The linear range between 1-50 μg of lysate makes it ideal for normalization of low-expressed proteins that require high loads of protein to reach the sensitivity for the detection of the target. TotalStain Q solves the issue of housekeeping proteins that lack linearity and normalization accuracy at high protein loads.

 

  • Simple 24-minute dye-and-wash protocol
  • Wide linear dynamic range across lysates of various tissue types
  • Compatible with both chemiluminescent and NIR fluorescence
HeLa-lysate-5μg-replicates-imaged-using-TotalStain-Q
HeLa lysate 10μg replicates imaged using TotalStain Q (Cy3 channel, green) and pSTAT3 on an Azure Sapphire Imager.

Documents

Document TypeDescription
BrochureAzure Total Protein Stains BrochureDOWNLOAD
BrochureTotalStain Q – NC Total Protein Stain KitDOWNLOAD
Safety Data SheetTotalStain Q - NC Stain Solution SDSDOWNLOAD
Safety Data SheetTotalStain Q - Wash Solution SDSDOWNLOAD
ProtocolTotalStain Q NC Short ProtocolDOWNLOAD
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