Radiance Plus

SKU: AC2103

$351.38 (USD)

The most sensitive HRP substrate available for chemiluminescent Western blotting

With attomole sensitivity and a long-lasting signal, Radiance Plus allows you to detect bands not visualized with other substrates. High signal-to-noise and a large dynamic range make it ideal for quantifying low-intensity bands.

Size: 150 mL

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Description

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[tabs style=”default”] [tab title=”Product Description”]

Chemiluminescent substrate for the most sensitive Western blotting experiments

Radiance Plus is the most sensitive HRP substrate available from Azure for chemiluminescent Western blotting. With attomole sensitivity and a long-lasting signal, Radiance Plus allows you to detect bands not visualized with other substrates. High signal-to-noise and a large dynamic range make it ideal for quantifying low-intensity bands.

• Low abundance protein detection
• Compare performance to SuperSignal West Femto and Amersham ECL Select
• Sensitivity in low femtograms
• Signal duration 6-8 hours

Size: 150mL

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[/tab][tab title=”Product Literature”]

Product Literature

Download PDFAzure Biosystems Western Blotting Workflow

Application Note – Why You Should Leave the Darkroom

Application Note – Chemiluminescent and Fluorescent Westerns: Choose the Best Assay for Your Experiment

Application Note – How to Improve Your Chemiluminescent Western Blots
[/tab][tab title=”Protocols & Product Inserts”]

Protocols & Product Inserts

Radiance Plus Short Protocol

Radiance Plus Long Protocol
[/tab][tab title=”Safety Data Sheets”]

Safety Data Sheets

Radiance Plus SDS

Download PDFRadiance and Radiance Plus Peroxide SDS
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features

  • Low abundance protein detection
  • Compare performance to competitor products, like SuperSignal West Femto and Amersham ECL Select
  • Sensitivity in low femtograms
  • Signal duration 6-8 hours
Showing sensitivity of Radiance Plus. Serial dilutions of HRP-conjugated antibody after incubation with Radiance Plus
The highest sensitivity. A slot blot containing serial dilutions of an HRP-conjugated antibody was incubated with Radiance Plus and detected with a 2-minute exposure time.
Radiance Plus exposure duration
Image blots hours after substrate incubation. A slot blot containing a serial dilution of an HRP-conjugated antibody was incubated with Radiance Plus and imaged at the times indicated. After 8 hours, the bands are still easily detected with only a 2-minute exposure.

citations

documents

CatFolders

Title Type Size Updated Link
Document TypeDescription
BrochureAzure Biosystems Western Blotting WorkflowDOWNLOAD
Application NotesWhy You Should Leave the DarkroomDOWNLOAD
Application NoteChemiluminescent and Fluorescent Westerns: Choose the Best Assay for Your ExperimentDOWNLOAD
Application NoteHow to Improve Your Chemiluminescent Western BlotsDOWNLOAD
ProtocolRadiance Plus Short ProtocolDOWNLOAD
ProtocolRadiance Plus Long ProtocolDOWNLOAD
Safety Data SheetRadiance Plus SDSDOWNLOAD
Safety Data SheetRadiance Peroxide SDSDOWNLOAD

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