Radiance ECL is an HRP substrate with long-lasting signal and sensitivity down to low picograms. Use up to 10 times less antibody than other substrates used in life science.
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The most common method for Western blot detection is chemiluminescence. Detection by chemiluminescence is relatively sensitive and works well for detecting individual or low abundance proteins.
Fluorescence Western blotting is beneficial when your experiment requires quantitation, particularly between different proteins in the same sample. Fluorescent methods are especially useful for detection of post-translationally modified versions of proteins, since the difference in molecular weight is often not enough for clear resolution on an average gel.
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|Brochure||Azure Biosystems Western Blotting Workflow||DOWNLOAD|
|Application Note||Why You Should Leave the Darkroom||DOWNLOAD|
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|Protocol||Radiance ECL Short Protocol||DOWNLOAD|
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