Azure Biosystems
$281.00 (USD)
Chemiluminescent HRP substrate for chemiluminescent Westerns
Radiance ECL is an HRP substrate with long-lasting signal and sensitivity down to low picograms. Use up to 10 times less antibody than other ECL substrates.
Product code: AC2204
The most common method for Western blot detection is chemiluminescence. Detection by chemiluminescence is relatively sensitive and works well for detecting individual or low abundance proteins.
Fluorescence Western blotting is beneficial when your experiment requires quantitation, particularly between different proteins in the same sample. Fluorescent methods are especially useful for detection of post-translationally modified versions of proteins, since the difference in molecular weight is often not enough for clear resolution on an average gel.
| Document Type | Description | |
|---|---|---|
| Brochure | Which Substrate is Right for You? | DOWNLOAD |
| Brochure | Azure Biosystems Western Blotting Workflow | DOWNLOAD |
| Application Note | Why You Should Leave the Darkroom | DOWNLOAD |
| Application Note | Chemiluminescent vs Fluorescent Westerns | DOWNLOAD |
| Application Note | How to Improve Your Chemiluminescent Western Blots | DOWNLOAD |
| Protocol | Radiance ECL Short Protocol | DOWNLOAD |
| Protocol | Radiance Substrates Long Protocol | DOWNLOAD |
| Safety Data Sheet | Radiance ECL SDS | DOWNLOAD |
| Safety Data Sheet | Radiance Peroxide SDS | DOWNLOAD |
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