HRP Stripping Buffer

SKU: AC2154

$152.75 (USD)

Works by removing the primary and secondary antibodies from the membrane

Stripping buffer for stripping and reprobing chemiluminescent Western blots. Provided as a 1x ready-to-use solution

  • Size: 500mL

View associated documents >

How does stripping buffer work?

Description

Stripping buffer for chemiluminescent Western blots

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features

Uniformly removes antibodies from developed HRP Western blots so the blot can be reprobed with another set of antibodies.

Western blot comparison showing stripping and reprobing results (right)
Blot was probed for phospho-STAT1 then stripped and reprobed for STAT1 using Azure Stripping Buffer. Lanes: Ladder, 1) 10 μg untreated HeLa lysate, 2) 10 μg IFNα-treated, 3) 20 μg untreated, 4) 20 μg IFNα-treated.

documents

Document TypeDescription
BrochureAzure Biosystems Western Blotting WorkflowDOWNLOAD
Application NotePhosphorylated Protein Detection by Chemiluminescent and Fluorescent Western BlotDOWNLOAD
Application NoteWhy You Should Leave the DarkroomDOWNLOAD
Application NoteChemiluminescent and Fluorescent Westerns: Choose the Best Assay for Your ExperimentDOWNLOAD
BrochureHow to Improve Your Chemiluminescent Western BlotsDOWNLOAD
ProtocolAzure HRP Stripping Buffer Short ProtocolDOWNLOAD
Safety Data SheetAzure HRP Stripping Buffer SDSDOWNLOAD
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