AzureSpectra Fluorescent Antibody Labeling Kits

$345.00 (USD)

Fast and simple antibody labeling. AzureSpectra Labeling kits provide all the materials needed to quickly label your primary and secondary antibodies with fluorescent dyes. Choose between AzureSpectra dyes 490, 550, 650, 700 or 800.

AzureSpectra Labeling Kits include enough reagents to label 100 μg of antibody (2 reactions of 50 μg each):

  • Antibody Labeling Buffer
  • Dye Solution
  • Quenching Solution
  • Neutralization Buffer
  • 2 G-25 Spin Columns

Description

[tabs style=”default”] [tab title=”Product Description”]

Fast and simple antibody labeling

AzureSpectra Labeling kits provide all the materials needed to quickly label your primary and secondary antibodies with fluorescent dyes. Choose between AzureSpectra dyes 490, 550, 650, 700 or 800.

Label your own validated primary or secondary antibodies to perform direct or traditional Westerns. Labeled antibodies are compatible with multiple immunofluorescent platforms, including flow cytometry, immunofluorescent microscopy and Western blotting.

Labeling Kits include enough reagents to label 100 μg of antibody (2 reactions of 50 μg each).

Each kit includes:

    Antibody Labeling Buffer
    Dye Solution
    Quenching Solution
    Neutralization Buffer
    2 G-25 Spin Columns

Demo kit incubation

 

Demo kit dyes

[/tab][tab title=”Product Literature”]

Product Literature

Download PDFAzureSpectra Labeling Kit Brochure

Download PDFAzure Biosystems Western Blotting Workflow

Download PDFApplication Note – Chemiluminescent and Fluorescent Westerns: Choose the Best Assay for Your Experiment

Download PDFApplication Note – How to Improve Your Fluorescent Western Blots

Download PDFApplication Note – Three Color Western Blots with AzureSpectra Antibodies

Download PDFApplication Note – Phosphorylated Protein Detection by Fluorescent Western Blot

[/tab][tab title=”Protocols & Product Inserts”]

Protocols & Product Inserts

Download PDFAzureSpectra Labeling Kit Short Protocol

[/tab][tab title=”Safety Data Sheets”]

Safety Data Sheets

Download PDFAzure Antibody Labeling Solution SDS

Download PDFAzureSpectra Dye Solution SDS

Download PDFAzure Neutralization Buffer SDS

Download PDFAzure Quenching Solution SDS[/tab]

[/tabs]

Additional information

Dye

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Available Options

AC2185490For covalently labeling antibodies with a fluorescent dye. Includes Labeling Buffer, dye solution, quenching solution, neutralization buffer and two G-25 columns. Each kit includes sufficient reagents to perform two labelings of 50 micrograms of antibody.$345.00 (USD)
AC2186550For covalently labeling antibodies with a fluorescent dye. Includes Labeling Buffer, dye solution, quenching solution, neutralization buffer and two G-25 columns. Each kit includes sufficient reagents to perform two labelings of 50 micrograms of antibody.$345.00 (USD)
AC2187650For covalently labeling antibodies with a fluorescent dye. Includes Labeling Buffer, dye solution, quenching solution, neutralization buffer and two G-25 columns. Each kit includes sufficient reagents to perform two labelings of 50 micrograms of antibody.$345.00 (USD)
AC2188700For covalently labeling antibodies with a fluorescent dye. Includes Labeling Buffer, dye solution, quenching solution, neutralization buffer and two G-25 columns. Each kit includes sufficient reagents to perform two labelings of 50 micrograms of antibody.$345.00 (USD)
AC2189800For covalently labeling antibodies with a fluorescent dye. Includes Labeling Buffer, dye solution, quenching solution, neutralization buffer and two G-25 columns. Each kit includes sufficient reagents to perform two labelings of 50 micrograms of antibody.$345.00 (USD)

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features

Label your own validated primary or secondary antibodies to perform direct or traditional Western blots. Labeled antibodies are compatible with multiple immunofluorescent platforms, including flow cytometry, immunofluorescent microscopy and Western blotting.

  • Easy and fast—The fluorescent antibody labeling kit provides the materials necessary to fluorescently label the antibody of your choice in less than 1 hour
  • Label primary antibodies—use antibodies that your lab has already
    validated and optimized.
  • Labeled primary antibodies can then be used for direct Westerns.
  • Label secondary antibodies—secondary antibodies can be labeled for traditional indirect Western blot applications
  • Choose from 5 dyes—the non-overlapping emission spectra of the
    fluorophores available for labeling means you can easily perform multiplexing
Simple antibody labeling protocol
AzureSpectraExcitation
maximum (nm)
Emission
Maximum (nm)
Dye-490491515
Dye-550551565
Dye-650653672
Dye-IR700690709
Dye-IR800690800
Perform multiplexing assays quick and easily. Fluorescent Western blot imaged with Azure c600. Secondary antibodies were labeled with the AzureSpectra labeling kit: anti-rabbit with AzureSpectra 700, anti-mouse with AzureSpectra 550, and anti-rat with AzureSpectra 800. Primary antibodies used were rat anti-tubulin, rabbit anti-beta actin, and mouse anti-GAPDH.

citations

documents

Document TypeDescription
BrochureAzureSpectra Labeling Kit BrochureDOWNLOAD
BrochureAzure Biosystems Western Blotting WorkflowDOWNLOAD
Application NoteChemiluminescent and Fluorescent Westerns: Choose the Best Assay for Your ExperimentDOWNLOAD
Application NoteHow to Improve Your Fluorescent Western BlotsDOWNLOAD
Application NoteThree Color Western Blots with AzureSpectra AntibodiesDOWNLOAD
Application NotePhosphorylated Protein Detection by Fluorescent Western BlotDOWNLOAD
ProtocolAzureSpectra Labeling Kit Short ProtocolDOWNLOAD
Safety Data SheetAzure Antibody Labeling Solution SDSDOWNLOAD
Safety Data SheetAzureSpectra 490 Dye Solution SDSDOWNLOAD
Safety Data SheetAzureSpectra 550 Dye Solution SDSDOWNLOAD
Safety Data SheetAzureSpectra 650 Dye Solution SDSDOWNLOAD
Safety Data SheetAzureSpectra 700 Dye Solution SDSDOWNLOAD
Safety Data SheetAzureSpectra 800 Dye Solution SDSDOWNLOAD
Safety Data SheetAzure Neutralization Buffer SDSDOWNLOAD
Safety Data SheetAzure Quenching Solution SDSDOWNLOAD

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Tel: (925) 307 7127
Fax: (925) 905-1816
Info@azurebiosystems.com

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