AzureSpectra Fluorescent Western Blotting Kits with Protein-free Block

$310.81 (USD)

Full kits for two-color fluorescent Western blots. Also offered with Fluorescent Block.

Each kit contains enough materials for 10 Western blots:

  • 9x7cm Low Fluorescence PVDF Membranes, 10 membranes
  • 10x Fluorescent Blot Washing Buffer, 250mL
  • 5x Protein-Free Blot Blocking Buffer, 60mL
  • 2x Background Quenching Sheets
  • 2x 40μL Fluorescent Secondary Antibodies

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Available Options

AC2140goat-α-rb IR700, goat-α-ms IR800$310.81 (USD)
AC2141goat-α-ms IR700, goat-α-rb IR800$310.81 (USD)
AC2104goat-α-rb 650, goat-α-ms 550$310.81 (USD)

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Each kit includes:

  • Pre-cut membranes,
  • Wash buffer,
  • Protein-free blot blocking buffer,
  • Quenching sheets,
  • Secondary antibodies to detect two proteins per experiment.
How two-color fluorescent multiplex Western blotting works
Two-color fluorescent multiplex Western blotting. Using secondary antibodies labeled with different fluorophores, two proteins can be detected on a single blot by controlling excitation and detection channels (a). AzureSpectra conjugates incorporate the phycobiliproteins allophycocyanin (APC) and R-phycoerythrin (RPE), extremely bright fluorescent proteins from cyanobacteria and aukaryotic algea (b,c). AzureSpectra IR conjugates incorporate IR700 and IR800 fluorescent dyes with near-infrared excitation and emission wavelengths (d,e).



Document TypeDescription
BrochureAzure Biosystems Western Blotting WorkflowDOWNLOAD
BrochureTips for Transitioning from Chemiluminescent to Fluorescent Western BlotsDOWNLOAD
Application NoteBlocking: Key to Optimizing Your Western BlotsDOWNLOAD
Application NoteChemiluminescent and Fluorescent Westerns: Choose the Best Assay for Your ExperimentDOWNLOAD
Application NoteHow to Improve Your Fluorescent Western BlotsDOWNLOAD
Application NoteThree Color Western Blots with AzureSpectra AntibodiesDOWNLOAD
Application NotePhosphorylated Protein Detection by Fluorescent Western BlotDOWNLOAD
ProtocolAzureSpectra 550/650 Western Kit with Protein-Free Block Short ProtocolDOWNLOAD
ProtocolAzureSpectra 700/800 Western Kit with Protein-Free Block Short ProtocolDOWNLOAD
Safety Data SheetAzure Protein-Free Blot Blocking Buffer SDSDOWNLOAD
Safety Data SheetAzure Fluorescent Blot Washing Buffer SDSDOWNLOAD
Safety Data SheetAzureSpectra 550 Secondary Antibodies SDSDOWNLOAD
Safety Data SheetAzureSpectra 650 Secondary Antibodies SDSDOWNLOAD
Safety Data SheetAzureSpectra 700 Secondary Antibodies SDSDOWNLOAD
Safety Data SheetAzureSpectra 800 Secondary Antibodies SDSDOWNLOAD
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