AzureRed Fluorescent Total Protein Stain

SKU: AC2124

$208.44 (USD)

Quantitative fluorescent total protein stain for gels and Western blots

For use with Western blot samples of biological fluids with very low protein concentration, 0.1-1μg lysate/lane.​ Choose AzureRed Fluorescent Protein Stain for sensitive detection of total protein on 1D or 2D gels. AzureRed is as sensitive as silver stain and is compatible with downstream Western blotting, mass spectrometry, and Edman sequencing. Because the free dye is non-fluorescent, AzureRed provides very low background and higher signal to noise than other fluorescent stains. AzureRed-stained gels can be imaged with UV or blue light excitation.

Allows you to detect less than 1 ng of protein per band or spot. Non-toxic and biodegradable, for safe and simple disposal. Provided as a 200x concentrated solution.

Each kit contains:

  • 5mL AzureRed Fluorescent Protein Stain
  • AzureRed Powder A, for fix solution preparation
  • AzureRed Powder B, for stain buffer preparation

View associated documents >

Description

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Total protein stain for gels and blots

Detect less than 1 ng of protein per band or spot with AzureRed Fluorescent Protein Stain. AzureRed is a quantitative, fluorescent protein stain for total protein normalization in blots and total protein detection in gels. It is fully compatible with downstream Western blotting or mass spectrometry. Stained gels and blots can be imaged on both laser- and CCD-based fluorescent imaging systems. AzureRed is non-toxic and biodegradable, for safe and simple disposal.

Features

• Simple staining protocol does not alter your proteins and requires minimal optimization
• Signal is reproducible across various tissue types and is unaffected by experimental conditions
• Wide linear dynamic range (over 4 orders of magnitude) increases quantitative accuracy
• Compatible with both fluorescent and chemiluminescent Western blotting

[/tab][tab title=”Product Literature”]

Product Literature

Download PDFAzure Total Protein Stains Brochure

Download PDFAzureRed Fluorescent Protein Stain Brochure

Download PDFApp Note – Accurate Western Blot Normalization with AzureRed Fluorescent Protein Stain

Download PDFAzure Biosystems Western Blotting Workflow
[/tab][tab title=”Protocols & Product Insert”]

Protocols & Product Inserts

Download PDFAzureRed Total Protein Membrane Staining Protocol

Download PDFAzureRed Short Protocol

Download PDFAzureRed Long Protocol
[/tab][tab title=”Safety Data Sheets”]

Safety Data Sheets

Download PDFAzureRed Fluorescent Protein Stain SDS

Download PDFAzureRed Powder A SDS

Download PDFAzureRed Powder B SDS
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features

AzureRed is a quantitative, fluorescent protein stain for total protein normalization in blots and total protein detection in gels. It is fully compatible with downstream Western blotting or mass spectrometry. Stained gels and blots can be imaged on both laser- and CCD-based fluorescent imaging systems.

Non-toxic and biodegradable, for safe and simple disposal

  • Simple staining protocol does not alter your proteins and requires minimal optimization
  • Signal is reproducible across various tissue types and is unaffected by experimental conditions
  • Wide linear dynamic range (over 4 orders of magnitude) increases quantitative accuracy
  • Compatible with both fluorescent and chemiluminescent Western blotting

Stain 1D and 2D gels: AzureRed stains 1D and 2D gels in less than 3 hours, with high sensitivity, low background, and no speckling.

AzureRed showed superior correlation and a much broader dynamic range than the common housekeeping proteins.
AzureRed showed superior correlation and a much broader dynamic range than the common housekeeping proteins, such as GAPDH.
Overlay of four channels. Western blot stained with total protein stain, AzureRed, probed for three proteins of interest without a destaining step, scanned with Azure Sapphire Biomolecular Imager
AzureRed is imaged simultaneously with three proteins of interest. The gel was loaded with dilutions of HeLa cell lysate. After transfer, the blot was stained with AzureRed and then probed for tubulin, ß-actin, and GAPDH without a destaining step. The blot was scanned with each of the four lasers of the Sapphire Biomolecular Imager. In this overlay of the four channels, total protein (AzureRed stain) is shown in gray; tubulin in red, ß-actin in blue, and GAPDH in green.

Documents

Document TypeDescription
BrochureAzure Total Protein Stains BrochureDOWNLOAD
BrochureAzureRed Fluorescent Protein Stain BrochureDOWNLOAD
Application NoteAccurate Western Blot Normalization with AzureRed Fluorescent Protein StainDOWNLOAD
BrochureAzure Biosystems Western Blotting WorkflowDOWNLOAD
ProtocolAzureRed Total Protein Membrane Staining ProtocolDOWNLOAD
ProtocolAzureRed Short ProtocolDOWNLOAD
ProtocolAzureRed Long ProtocolDOWNLOAD
Safety Data SheetAzureRed Fluorescent Protein Stain SDSDOWNLOAD
Safety Data SheetAzureRed Powder A SDSDOWNLOAD
Safety Data SheetAzureRed Powder B SDSDOWNLOAD

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