The AzureCyto In-Cell Western Kit contains all reagents necessary for staining of whole cells for total cell normalization and simultaneous detection of two biomarkers. The AzureCyto Permeabilization Solution removes membrane lipids and facilitates permeabilization of the nuclear membrane to allow the penetration of antibodies into cells for the detection of intracellular and nuclear proteins.
Can be co-incubated with primary antibodies to streamline the detection process and reduce the number of assay steps. Each kit includes secondary antibodies conjugated to AzureSpectra 700 and AzureSpectra 800 to enable the simultaneous detection of two biomarkers. Includes all reagents necessary to perform five 96-well in-cell Western assays.
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|AC2022||Rabbit||AzureCyto In-Cell Western Kit with AzureSpectra 700 Goat anti-Rabbit and Azure Spectra 800 Goat anti-Mouse. Includes all reagent necessary to perform 5x 96-well In-Cell Western Assays with total cell staining. Includes AzureCyto Permeabilization Solution, AzureCyto Block Solution, AzureCyto Total Cell Stain, AzureSpectra 700 Goat anti-Rabbit and AzureSpectra 800 Goat anti-Mouse secondary antibodies.||$749.00 (USD)|
|AC2023||Mouse||AzureCyto In-Cell Western Kit with AzureSpectra 700 Goat anti-Mouse and Azure Spectra 800 Goat anti-Rabbit. Includes all reagent necessary to perform 5x 96-well In-Cell Western Assays with total cell staining. Includes AzureCyto Permeabilization Solution, AzureCyto Block Solution, AzureCyto Total Cell Stain, AzureSpectra 700 Goat anti-Mouse and AzureSpectra 800 Goat anti-Rabbit secondary antibodies.||$749.00 (USD)|
Unlock the full spectrum of cellular protein expression with AzureCyto Total Cell Stain’s linear response, ensuring precise quantification and robust comparisons.
AzureCyto Total Cell Stain makes normalization at low concentrations possible, allowing more meaningful comparisons of small amounts of protein and uncovering subtleties of cellular signaling.
Elevate your research with unparalleled accuracy, making every signal count.
Tailored for a wide range of cell types and experimental conditions, AzureCyto Total Cell Stain adapts to your unique research needs.
|Short Protocol||AzureCyto In-cell Western Kit Short Protocol (Full Document)||DOWNLOAD|
|SDS||AzureCyto Permeabilization Solution Safety Data Sheet||DOWNLOAD|
|SDS||AzureCyto Blocking Buffer SDS||DOWNLOAD|
|SDS||AzureCyto Total Cell Stain SDS||DOWNLOAD|
|App Note||Using Total Cell Staining and Advanced Imaging to Unlock the Full Power of In-Cell Westerns||DOWNLOAD|
|Flyer||Accurate and Reliable In-Cell Westerns with for Biomarker Discovery using AzureCyto||DOWNLOAD|