AzureCyto In-Cell Western Kit

$749.00 (USD)

The AzureCyto In-Cell Western Kit contains all reagents necessary for staining of whole cells for total cell normalization and simultaneous detection of two biomarkers. The AzureCyto Permeabilization Solution removes membrane lipids and facilitates permeabilization of the nuclear membrane to allow the penetration of antibodies into cells for the detection of intracellular and nuclear proteins.

Can be co-incubated with primary antibodies to streamline the detection process and reduce the number of assay steps. Each kit includes secondary antibodies conjugated to AzureSpectra 700 and AzureSpectra 800 to enable the simultaneous detection of two biomarkers. Includes all reagents necessary to perform five 96-well in-cell Western assays.

Includes:

  • AzureCyto Permeabilization Solution: removes membrane lipids and facilitates permeabilization of the nuclear membrane to allow the penetration of antibodies into cells for the detection of intracellular and nuclear proteins
  • AzureCyto Block Solution: Blocking solution formulated for cell-based assays and fluorescence detection
  • AzureCyto Total Cell Stain: A sensitive and linear stain that can be co-incubated with primary antibodies to streamline the detection process and reduce the number of assay steps
  • AzureSpectra Secondary Antibodies: For simultaneous detection of two biomarkers

View associated documents >

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Available Options

AC2022RabbitAzureCyto In-Cell Western Kit with AzureSpectra 700 Goat anti-Rabbit and Azure Spectra 800 Goat anti-Mouse. Includes all reagent necessary to perform 5x 96-well In-Cell Western Assays with total cell staining. Includes AzureCyto Permeabilization Solution, AzureCyto Block Solution, AzureCyto Total Cell Stain, AzureSpectra 700 Goat anti-Rabbit and AzureSpectra 800 Goat anti-Mouse secondary antibodies.$749.00 (USD)
AC2023MouseAzureCyto In-Cell Western Kit with AzureSpectra 700 Goat anti-Mouse and Azure Spectra 800 Goat anti-Rabbit. Includes all reagent necessary to perform 5x 96-well In-Cell Western Assays with total cell staining. Includes AzureCyto Permeabilization Solution, AzureCyto Block Solution, AzureCyto Total Cell Stain, AzureSpectra 700 Goat anti-Mouse and AzureSpectra 800 Goat anti-Rabbit secondary antibodies.$749.00 (USD)

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features

1:2 serial dilution of HeLa cells seeded into a 96-well plate
1:2 serial dilution of HeLa cells seeded into a 96-well plate. (A) A composite image of three channels imaged simultaneously on the Sapphire™ FL at 100-micron resolution. (B) hnRNP K visualized with the 685 Standard Optical Module. (C) GAPDH visualized with the 784 Standard Optical Module. (D) AzureCyto stain visualized with the 532 Standard Optical Module. All images were taken on a Sapphire™ FL Biomolecular Scanner.

Linearity

Unlock the full spectrum of cellular protein expression with AzureCyto Total Cell Stain’s linear response, ensuring precise quantification and robust comparisons.

Figure 1. AzureCyto Total Cell Stain is linear over a broad range of cell volumes.

Sensitivity

AzureCyto Total Cell Stain makes normalization at low concentrations possible, allowing more meaningful comparisons of small amounts of protein and uncovering subtleties of cellular signaling.

Enhanced Accuracy

Elevate your research with unparalleled accuracy, making every signal count.

Versatility

Tailored for a wide range of cell types and experimental conditions, AzureCyto Total Cell Stain adapts to your unique research needs.

How to use

how to use the azurecyto in-cell western reagent kit

citations

Documents

Document TypeDescription
Short ProtocolAzureCyto In-cell Western Kit Short Protocol (Full Document)DOWNLOAD
Product Data SheetAzureCyto In-Cell Western Kit Product Data SheetDOWNLOAD
SDSAzureCyto Permeabilization Solution Safety Data SheetDOWNLOAD
SDSAzureCyto Blocking Buffer SDSDOWNLOAD
SDSAzureCyto Total Cell Stain SDSDOWNLOAD
App NoteUsing Total Cell Staining and Advanced Imaging to Unlock the Full Power of In-Cell WesternsDOWNLOAD
FlyerAccurate and Reliable In-Cell Westerns with for Biomarker Discovery using AzureCytoDOWNLOAD
FlyerHow In-Cell Westerns Can Advance Your ResearchDOWNLOAD
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