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Washing buffer optimized for use with fluorescent secondary antibodies
Optimized for use with both visible and near-infrared fluorescent Western blots, Azure’s Fluorescent Blot Washing Buffer is specially formulated to reduce non-specific binding of fluorescent secondary antibodies to produce clean blots with high signal-to-noise ratios.
Washing is an important step during Western blotting to remove unhybridized antibody from the membrane. Azure’s Fluorescent Washing Buffer is provided as a 10x concentrate that can be diluted in water.
Provided as a 10x solution
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Azure Biosystems Western Blotting Workflow
Tips for Transitioning from Chemiluminescent to Fluorescent Western Blots
Application Note – Chemiluminescent and Fluorescent Westerns: Choose the Best Assay for Your Experiment
Application Note – How to Improve Your Fluorescent Western Blots
Application Note – Three Color Western Blots with AzureSpectra Antibodies
Application Note – Phosphorylated Protein Detection by Fluorescent Western Blot
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Safety Data Sheets
Fluorescent Blot Washing Buffer SDS