Fluorescent Blot Blocking Buffer

SKU: AC2190

$181.13 (USD)

Blot block that stabilizes fluorescent signal

Azure Fluorescent Blot Blocking Buffer works quickly for shorter membrane incubation times. Effectively prevents non-specific binding, and stabilizes secondary antibody conjugate fluorescence.

  • Provided as a 1x Ready-to-Use solution
  • Size: 500mL

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Azure Fluorescent Blot Blocking Buffer is optimized for use with the Azure fluorescent Western blotting systems. It is formulated to lower background noise and to stabilize fluorescent signal.

Western blot membranes should be incubated in blocking buffer before antibodies are added to prevent non-specific binding. Blocking buffer can also be used for diluting primary and secondary antibodies during hybridization.

High noise fluorescent Western blot
Figure 1. Choice of blocking agent can be the difference between high background (A) and excellent signal-to-noise ratios (B). Duplicate dot blots were processed and imaged identically except for the blocking step which was either 5% nonfat dry milk in TBST (A) or Azure Protein-Free Blot Blocking Buffer (B).


Document TypeDescription
BrochureAzure Biosystems Western Blotting WorkflowDOWNLOAD
Short ProtocolFluorescent Blot Blocking Buffer Short ProtocolDOWNLOAD
BrochureTips for Transitioning from Chemiluminescent to Fluorescent Western BlotsDOWNLOAD
Application NoteBlocking: Key to Optimizing Your Western BlotsDOWNLOAD
Application NoteChemiluminescent and Fluorescent Westerns: Choose the Best Assay for Your ExperimentDOWNLOAD
Application NoteHow to Improve Your Fluorescent Western BlotsDOWNLOAD
Application NoteThree Color Western Blots with AzureSpectra AntibodiesDOWNLOAD
Application NotePhosphorylated Protein Detection by Fluorescent Western BlotDOWNLOAD
Safety Data SheetAzure Fluorescent Blot Blocking Buffer SDSDOWNLOAD
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