While fluorescent and chemiluminescent imaging techniques and their detectors are becoming more sensitive and selective, photo-stimulated luminescence from phosphor storage plates remains a key application for many researchers. The Sapphire’s ability to phosphor image comes in handy especially for applications such as electrophoretic mobility shift assays (EMSA), enzyme assays, and in-vivo imaging.
The Sapphire is a compact phosphor imager incorporates exceptional visible fluorescent and near infra-red scanning along with true chemiluminescent and white light imaging, making it a true lab workhorse.
Phosphor imaging is an excellent option for quantitative comparisons of radiolabels. Crystals in the storage phosphor screen absorb energy emitted by radioactive substances. When excited by the Sapphire’s laser, the screen emits light proportionate to the original radioactivity. Phosphor imaging provides a much greater linear dynamic range than X-ray film when detecting radiolabels.
The figures to the right demonstrate the linearity and wide dynamic range obtained when using phosphor imaging on the Sapphire. When imaging a carbon-14 standard, the linear dynamic range was greater than 5 orders of magnitude. Phosphor imaging can show the location of radioactivity within a tissue section or sample (Figure 1), allowing you to follow incorporation of radioactive labels or tracers.
In addition to high-quality phosphor imaging, the Sapphire Biomolecular Imager is capable of multicolor fluorescence, NIR, chemiluminescent, and white light imaging. Image gels, blots, tissues slices and more
As scientists, we like to pride ourselves on objectively evaluating data before coming to any conclusions, and staying open to new ideas if they are better supported by new information. Purchasing a new piece of lab equipment should be no different, especially when it comes to instruments that we entrust with making measurements and record data for our critical studies.
Doing the research to find a new piece of equipment and ensuring that it meets your lab’s needs for quality and capabilities can be time-consuming, and is probably not the type of research you had in mind when you became a scientist. Let our team of imaging experts help you find the right laser scanner for your studies.
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