Total Flexibility with Phosphor Imaging

Sapphire FL: the Ultimate Phosphor Imager

While fluorescent and chemiluminescent imaging techniques and their detectors are becoming more sensitive and selective, photo-stimulated luminescence from phosphor storage plates remains a key application for many researchers. The Sapphire FL’s ability to phosphor image comes in handy especially for applications such as electrophoretic mobility shift assays (EMSA), enzyme assays, and in vivo imaging.

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Effortlessly Scan screens for filmless autoradiography

High-quality phosphor images, large dynamic range

The Sapphire FL is a compact* phosphor imager that incorporates exceptional visible fluorescent and near infra-red scanning along with true chemiluminescent and white light imaging, making it a true lab workhorse.

*Sapphire FL has a size of 63.0 cm x 39.9 cm x 59.3 cm / 24.8” x 15.7” x 23.3″.

Phosphor Imaging done on Azure Sapphire Biomolecular Imager

quantiative comparisons of radiolabels

Sapphire FL scans storage phosphor screens with exceptional dynamic range and image quality, thanks to the use of laser excitation and photon multiplier tube (PMT) detection.

Phosphor imaging is an excellent option for quantitative comparisons of radiolabels. Crystals in the storage phosphor screen absorb energy emitted by radioactive substances. When excited by the Sapphire’s laser, the screen emits light proportionate to the original radioactivity. Phosphor imaging provides a much greater linear dynamic range than X-ray film when detecting radiolabels.

The figures to the right demonstrate the linearity and wide dynamic range obtained when using phosphor imaging on the Sapphire. When imaging a carbon-14 standard, the linear dynamic range was greater than 5 orders of magnitude. Phosphor imaging can show the location of radioactivity within a tissue section or sample (Figure 1), allowing you to follow incorporation of radioactive labels or tracers.

American Radiolabel Chemicals Carbon-14 standard exposed to Storage Phosphor Screen for three hours, then imaged with the Azure Sapphire Biomolecular Imager
Figure 1. American Radiolabel Chemicals Carbon-14 standard exposed to storage phosphor screen for three hours, then imaged with the Azure Sapphire Biomolecular Imager
Sample: C autoradiographic standard; Imaging; Phosphor; LOD: 0.036 µCi/g; DR: 5.4 orders of magnitude; Linearity: R²=0.99
Plot showing linearity and linear dynamic range achieved when a carbon-14 standard was imaged using the Sapphire

exceptional dynamic range and image quality

Exceptional visible fluorescent and near infra-red scanning

In addition to high-quality phosphor imaging, the Sapphire FL is capable of multicolor fluorescence, NIR, white light imaging, and chemiluminescence imaging with the addition of the Chemiluminescent Module. Almost endless possibilities of laser and filter combinations allow you to image live animals, gels, Western blots, tissues slices, and more.

As scientists, we like to pride ourselves on objectively evaluating data before coming to any conclusions, and staying open to new ideas if they are better supported by new information. Purchasing a new piece of lab equipment should be no different, especially when it comes to instruments that we entrust with making measurements and record data for our critical studies.

Three Whole Mice - scanned at 10μm with the Sapphire
Mouse embryo on Sapphire

data integrity and reproducibility matter

It’s critical the instruments that generate your data are reliable.

Doing the research to find a new piece of equipment and ensuring that it meets your lab’s needs for quality and capabilities can be time-consuming, and is probably not the type of research you had in mind when you became a scientist. Let our team of imaging experts help you find the right laser scanner for your studies.

Azure Sapphire FL Biomolecular Imager with lid open
The Sapphire FL Biomolecular Imager is capable of high-resolution imaging and wide depth of field enable many sample types, including arrays, microarrays, Western blots, tissue slides, and small animals.

Sapphire FL Applications

  • Quantitative Western blotting
  • NIR Western blotting
  • Chemiluminescence
  • Protein gel documentation
  • DNA gel documentation
  • In-gel imaging/in-gel Westerns
  • Protein array imaging
  • EMSA
  • 2D-DIGE
  • Reporter gene assays
  • Tissue imaging/Tissue section imaging
  • Small model organism imaging
  • Phosphor imaging
  • In-cell/on-cell ELISA
  • 2D DNA gels
  • Densitometry
  • Northern blots
  • Southern blots
  • MORE
Fluorescent Western blots with up to four-color detection
Multiplex fluorescent Western blot from Azure Biosystems imager

Get faster workflows and more reliable quantitation

Track protein movement through tissue in small animals

Lymphatic antigen tracking in mouse hindpaw

Sensitive chemiluminescent detection
Chemiluminescent Western blot with color marker imaged with Azure Biosystems

Maximize your Western blot workflow options

View and quantify 35S-labeled proteins in 2D gels

Perform proteomics analysis on metabolically-labeled samplesPerform proteomics analysis on metabolically-labeled samples

Scientists are Raving about Sapphire

Add phosphor imaging to your application aresenal.

See what the hype is about surrounding the Azure Sapphire FL. Let's chat.

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