# Azure Biosystems
> Azure Biosystems is a life science technology company specializing in imaging systems, qPCR instruments, and reagents for molecular biology research. Our solutions support applications such as western blotting, gel imaging, and nucleic acid analysis.

Azure Biosystems provides high-performance imaging and qPCR systems designed to deliver accurate, reproducible results for researchers in academic, clinical, and industrial laboratories worldwide.

## Products
- [Sapphire FL](https://azurebiosystems.com/products/sapphirefl/): Discover the Sapphire FL biomolecular imager for NIR fluorescence, RGB fluorescence, chemiluminescence, and phosphor imaging with modular upgrade flexibility.
- [Sapphire FL plus](https://azurebiosystems.com/products/sapphireflplus/): Large format scanner for precise quantitation of molecular assays
- [Sapphire Eraser](https://azurebiosystems.com/products/sapphire-eraser/): The Azure Sapphire Eraser is a dedicated instrument for erasing storage phosphor screens (imaging plates).
- [Cielo qPCR System](https://azurebiosystems.com/products/cielo/): Learn how the Cielo real-time PCR system supports accurate, reproducible, multiplex qPCR applications of gene expression, genotyping, thermal shift assay with no ROX requirement.
- [AzureSpot Pro](https://azurebiosystems.com/software/azurespotpro/): Use AzureSpot Pro for western blot quantification, molecular weight analysis, normalization, and image analysis across gels, blots, and plates.
- [chemiSOLO](https://azurebiosystems.com/products/chemisolo/): Compact chemiluminescent imager for quantitative chemiluminescent and visible protein gels
- [AzureSpot Q](https://azurebiosystems.com/software/azurespotq/): Software for Spot and Band Intensity and Molecular Weight Analysis
- [Ao Absorbance Microplate Reader](https://azurebiosystems.com/products/ao-absorbance-microplate-reader/): Filter-based 96-well plate absorbance microplate reader for ELISA, Bradford, Lowry assays & more
- [AzureSpectra Fluorescent Demo Kits](https://azurebiosystems.com/product/fluorescent-demo-kits/): Azure’s Fluorescent Demo Kits contain everything you need to perform a single-color fluorescent Western using your own primary antibody and protein sample of your choice
- [AzureSpectra Fluorescent Secondary Antibodies](https://azurebiosystems.com/product/azurespectra-fluorescent-secondary-antibodies/): Secondary antibodies labeled with fluorophores that emit light in visible and near-infrared wavelengths
- [8-Tube Strip 0.1 ml](https://azurebiosystems.com/product/8-tube-strip/): 0.1 mL tube strips are specially designed for qPCR applications and assays
- [Flat 8-Cap Strip](https://azurebiosystems.com/product/flat-8-cap-strip/): Cap strips designed to fit on most tube strips, and 96-well plates
- [Radiance Plus](https://azurebiosystems.com/product/radiance-plus/): The most sensitive HRP substrate available for chemiluminescent Western blotting
- [Radiance Q](https://azurebiosystems.com/product/radiance-q/): Radiance Q chemiluminescent substrate is specially formulated for use with Azure Imaging Systems, or other CCD imagers
- [Western Blot Incubation Boxes](https://azurebiosystems.com/product/western-blot-box/): Black Western blot boxes are great for light-sensitive applications, like near-infrared fluorescent and chemiluminescent Western blots or protein gels
- [TotalStain Q (PVDF)](https://azurebiosystems.com/product/totalstain-q-pvdf/): Accurate and quantitative fluorescent total protein stain to perform normalization of Western blots
- [Fluorescent Blot Blocking Buffer](https://azurebiosystems.com/product/azure-fluorescent-blot-blocking-buffer/): Blot block that stabilizes fluorescent signal
- [PVDF Membrane](https://azurebiosystems.com/product/pvdf-membrane/): Pre-cut PVDF membranes for Western blotting applications, ideal for chemiluminescent and colorimetric Western blotting

## Other Core Pages
- [Homepage](https://azurebiosystems.com/): Azure Biosystems company homepage.  Explore western blot imaging systems, qPCR instruments, cell counters, electrophoresis instruments, analysis software, and reagents designed for high-sensitivity, quantitative life science research.
- [Discover the Sapphire](https://azurebiosystems.com/campaigns/discover-the-sapphire-v2/): Overview of the Sapphire Biomolecular Imager, highlighting its capabilities for multiplex fluorescent imaging, high sensitivity detection, and streamlined workflows for western blotting and other protein analysis applications.
- [Gel Electrophoresis Steps](https://azurebiosystems.com/blog/gel-electrophoresis-steps/): Learn the main gel electrophoresis steps, what equipment you need, how to troubleshoot, and how to visualize and document results accurately.
- [Azure Imaging Systems](https://azurebiosystems.com/products/azure-imaging-systems/): Compare gel doc and western blot imaging systems with NIR fluorescence, visible fluorescence, and chemiluminescence for quantitative, publication-ready results.
- [6 Steps to Western Blotting](https://azurebiosystems.com/6-steps-to-western-blotting/): High-level workflow of western blotting, covering gel electrophoresis, membrane transfer, blocking, primary antibody incubation, secondary antibody incubation, and Western blot analysis.
- [Azure 600 Imaging System](https://azurebiosystems.com/products/azure-imaging-systems/azure-600/): Azure 600 delivers geldoc and chemidoc with two-channel NIR fluorescence and chemiluminescent imaging for sensitive, quantitative western blot workflows.
- [Azure 200 Imaging System](https://azurebiosystems.com/products/azure-imaging-systems/azure-200/): Entry-level chemiluminescent imaging system designed for sensitive western blot detection with a simple and compact workflow.
- [Azure 300 Imaging System](https://azurebiosystems.com/products/azure-imaging-systems/azure-300/): Mid-range imaging system combining chemiluminescent and visible detection for flexible protein and nucleic acid analysis.
- [Best Protein Assay Methods](https://azurebiosystems.com/blog/protein-assay-best/): Compare BCA, Bradford, and Lowry protein assays to find the best fit for your sample type, sensitivity needs, and workflow.
- [Western Blot Transfer Methods](https://azurebiosystems.com/blog/western-blot-transfer-methods/): Compare wet, semi-dry, and dry western blot transfer methods to choose the best option for speed, reagent use, and quantitative performance.
- [How SDS-PAGE Separates Proteins](https://azurebiosystems.com/blog/how-sds-page-separates-proteins/): Understand how SDS-PAGE separates proteins by size, how gel migration works, and why it is a critical first step before western blotting.

## Applications
- [2D DIGE](https://azurebiosystems.com/western-blotting-applications/2d-dige/): Two-dimensional gel electrophoresis (2D DIGE) facilitates the comparison of proteomes (all proteins in a sample) in order to identify differences in specific protein levels. In 2D DIGE, two or more samples are run on a single gel.
- [Arrays and Microarrays](https://azurebiosystems.com/western-blotting-applications/arrays-and-microarrays/): Arrays and microarrays offer a way to carry out hundreds, thousands, and even tens of thousands of miniature hybridization assays in parallel. Interactions between many types of molecules can be studied in an array format.
- [Bacterial Plate Imaging](https://azurebiosystems.com/western-blotting-applications/bacterial-plate-imaging/): Counting bacterial colonies is a great means of identifying the general bacteria content of a sample.
- [Chemiluminescent Western Blotting](https://azurebiosystems.com/western-blotting-applications/chemiluminescent-western-blotting/): Chemiluminescent Western blots are popular assays for assessing protein expression. In this indirect detection method, chemiluminescent substrates emit light when reacted with an antibody conjugated to an enzyme.
- [Customer-submitted Applications](https://azurebiosystems.com/western-blotting-applications/customer-submitted-applications/): Real-world examples of imaging applications submitted by researchers using Azure Biosystems instruments.
- [Densitometry](https://azurebiosystems.com/western-blotting-applications/densitometry/): Densitometry is the quantitative measurement of optical density. It is the process of quantitating sample amounts based on their absorbance of light.
- [ELISA](https://azurebiosystems.com/western-blotting-applications/elisa/): Get a clear overview of ELISA principles, workflow steps, and common research and diagnostic applications for protein and antigen detection.
- [Gel Documentation](https://azurebiosystems.com/western-blotting-applications/gel-documentation/): Gel documentation is a method used to record and measure labeled nucleic acid and protein in various types of media, like acrylamide, agarose, or cellulose.
- [In-cell Western](https://azurebiosystems.com/western-blotting-applications/in-cell-western/): In-cell Westerns (ICW) are an immunocytochemistry method that combines the specificity of Western blotting with the high-throughput of ELISA.
- [In-gel Fluorescence](https://azurebiosystems.com/western-blotting-applications/in-gel-fluorescence/): Fluorescent fusion proteins can easily be visualized without having to transfer to a membrane for traditional Western analysis.
- [Membrane Arrays](https://azurebiosystems.com/western-blotting-applications/membrane-arrays/): Membrane arrays offer a multiplex approach to assaying protein expression. They are membrane-based assays that allow simultaneous screening of multiple proteins in a sample.
- [Model Organism Imaging](https://azurebiosystems.com/western-blotting-applications/model-organism-imaging/): Document fluorescent protein expression in three-dimensional model organisms, such as xenopus oocytes or zebrafish, or collect multiplex fluorescent images of tissue sections.
- [Multiplex Fluorescent Western Blot](https://azurebiosystems.com/western-blotting-applications/multiplex-fluorescent-western-blot/): Fluorescent Western blotting uses secondary antibodies directly conjugated to fluorescent dyes.
- [Multiplex Protein Detection](https://azurebiosystems.com/western-blotting-applications/multiplex-protein-detection/): Multiplexing fluorescent Western blots (Figure 1), also referred to as multiplex fluorescence, refers to the “multiplex” ability to detect multiple proteins simultaneously on the same Western blot.
- [NIR Western Blotting](https://azurebiosystems.com/western-blotting-applications/nir-western-blotting/): Near-infrared Western blots (NIR) allow you to easily resolve and quantify co-migrating bands, such as phosphorylated and nonphosphorylated isoforms of a protein, or isoforms containing other post-translational modifications.
- [Northern Blotting](https://azurebiosystems.com/western-blotting-applications/northern-blotting/): Northern blotting is a technique used by molecular biologists to detect a specific RNA sequence in a sample.
- [On-cell Western](https://azurebiosystems.com/western-blotting-applications/on-cell-western/): The on-cell Western (OCW) assay is a whole-cell immunodetection assay used to monitor cell surface protein expression.
- [Phosphor Imaging](https://azurebiosystems.com/western-blotting-applications/phosphor-imaging/): Phosphor imaging is a method to detect radioactive material in applications such as Northern, Southern, or Western blotting, or in radiolabeled tissue sections.
- [Post-translational Modifications](https://azurebiosystems.com/western-blotting-applications/post-translational-modifications/): Post-translational modifications (PTMs) occur when proteins are synthesized, and many proteins are modified by the covalent addition of chemical groups or compounds to amino acid side chains.
- [Protein Quantitation](https://azurebiosystems.com/western-blotting-applications/protein-quantitation/): Total protein normalization (TPN) is a laboratory method used to resolve the native difficulties with linearity in the immunodetection of target and control proteins.
- [Southern Blotting](https://azurebiosystems.com/western-blotting-applications/southern-blotting/): Learn what Southern blotting is, how the method works, the main workflow steps, and which imaging systems are used for DNA detection.
- [Total Protein Normalization](https://azurebiosystems.com/western-blotting-applications/total-protein-normalization/): Total protein normalization (TPN) is a laboratory method used to resolve the native difficulties with linearity in the immunodetection of target and control proteins.
- [Visible Gel Imaging](https://azurebiosystems.com/western-blotting-applications/visible-gel-imaging/): Visible gel imaging is a straightforward means of detection after electrophoresis.
- [Western Blotting](https://azurebiosystems.com/western-blotting-applications/western-blotting/): Western blotting is a widely used analytical technique that can identify one or more specific proteins in a complex mixture of proteins.
- [Gene Expression](https://azurebiosystems.com/qpcr-applications/gene-expression/): Gene expression analysis measures mRNA levels to understand biological activity and regulation.
- [Genotyping](https://azurebiosystems.com/qpcr-applications/genotyping/): Genotyping involves identifying differences in genetic information between individuals and is important in multiple areas of research.
- [Library Quantification](https://azurebiosystems.com/qpcr-applications/library-quantification/): Next-generation sequencing (NGS) uses large-scale DNA sequencing technology to determine the sequence of DNA or RNA.
- [Pathogen Detection](https://azurebiosystems.com/qpcr-applications/pathogen-detection/): Pathogen detection is important in the diagnosis of disease.
- [qPCR](https://azurebiosystems.com/qpcr-applications/qpcr/): qPCR is used to quantify the number of copies of a given DNA sequence using PCR.
- [Multiplex qPCR](https://azurebiosystems.com/qpcr-applications/multiplex-qpcr/): Multiplex qPCR enables simultaneous detection of multiple targets in a single reaction.

## Resources
- [Blog](https://azurebiosystems.com/blog/): Azure Biosystems blog, press releases, scientific resources, and product guides
- [Application Notes](https://azurebiosystems.com/learn/application-technical-notes/): Educational content on imaging techniques and instruments
- [Publications](https://azurebiosystems.com/learn/publications/): Scientific publications that reference Azure Biosystems instruments and systems
- [Privacy Policy](https://azurebiosystems.com/about/privacy-policy/)

## Contact
- Address: 6747 Sierra Court, Suite A-B, Dublin, CA 94568
- Phone: (925) 307-7127
- Email: info@azurebiosystems.com
- [Contact Form](https://azurebiosystems.com/about/contact-us/)