Azure BiosystemsHigh-quality and affordable imaging systems, qPCR systems, and reagents.
Azure BiosystemsSpeaker: Dr. Thiago Soares de Souza Vieira, Postdoctoral Fellow, National Institutes of Health
Heme Oxygenase-1 (HO-1) protein translation is a widespread host skin response to the bite of many vectors of disease. See the importance of HO-1 to control tolerance of the host to both transmitted pathogens and tissue damage supported by full knockdown model and in vivo enzymatic inhibition of the enzyme. Experiments include ECL and Total Protein Normalization in Western Blots as a tool to evaluate changes in protein expression produced by insect bites using the Azure Biosystems c600 imager.
Speaker: Dr. Alfred Xuyang Sun, Junior Principal Investigator, Duke NUS
Angelman Syndrome (AS) is a neurodevelopmental disorder characterized by intellectual disability and frequent seizures. Despite clear genetic evidences that most AS cases are caused by loss-of-function mutations or deletions of UBE3A, an E3ubiquitin ligase, how the network hyperactivity observed in patients arises from UBE3A deficiency in individual neurons is still lacking.
Presented by Stanley Domijan, Field Application Scientist
Have you heard about Total Protein Normalization (TPN)? Publications are increasingly requiring the use of total protein signal for normalization because TPN offers several advantages over housekeeping protein normalization in Western Blot analysis. Come and let Azure Biosystems lead you through the wondrous world of TPN, and learn more about the principles and analysis!
Presented by Dr. Sida Shen, University of Illinois Chicago and Dr. Satish Noonepalle, The University of George Washington
Protein lysine acetylation and deacetylation regulate key pathways involving cellular processes, such as transcription, cell cycle, and metabolism. Lately, given by the immunomodulatory properties, selective HDAC6 inhibitors have gained prominence as potential therapeutic agents for cancer immunotherapy. In our recent article, we utilized in-silico simulations to design a novel HDAC6 inhibitor, named Suprastat, by targeting unique residues in the HDAC6 pocket but are absent in the other HDAC isoforms.
Presented by Dr. John Streicher, Assistant Professor, University of Arizona
Cannabis is widely popular for the treatment of chronic pain and other conditions, especially as legal hurdles continue to be lifted nationwide. Cannabinoids found in the plant like delta-9-tetrahydrocannabinol (THC) as well as whole plant extracts continue to be heavily researched for their ability to relieve chronic pain. However some studies suggest that THC only has modest efficacy in relieving pain, and THC and other cannabinoids cause unwanted psychoactive side effects.
Presented by Dr. KaiLeng Tan, Regional Field Applications Scientist
Do you know that a good experimental design is essential to produce publication quality and reproducible qPCR data? Let Azure Biosystems bring you back to the basics, and help you get a grasp of the essence of qPCR experimental design!
Highlights:
• Quality control with good experimental design
• Nucleic acid quality control
• Primer design tips and validation
Presented by, Dr. Tadatoshi Sato, Instructor, Harvard Medical School
Our skeleton is a dynamic tissue that continually adapts to external forces. During spaceflight, space pilots lose significant amounts of bone mass; conversely, physical exercise stimulates new bone formation. The pathways used by bone cells to sense external mechanical cues are poorly understood.
In our recent publication, we discovered a novel signaling pathway induced in osteocytes, cells placed within mineralized bone tissue, to respond to exercise. This work has important implications for the development of pharmacological approaches to boost skeletal responses to exercise in aging individuals.
Presented by Joy DeTorres, Application Scientist
Total protein normalization is the new standard required by journals for western blot quantification. Housekeeping proteins (HKPs) are widely used as an internal reference for western blot normalization. They are often highly expressed, lose proportionality at high protein loads, and only represent one portion of your whole sample. For this reason, using HKPs is an unreliable method of calculating normalization ratios.
TotalStain Q accurately accounts for lane-to-lane variations inherent in western blotting. With linearity up to 50 μg of lysate, TotalStain Q is ideal for the normalization of lowly expressed proteins that require high sample loads. Kits are available for both PVDF and nitrocellulose membranes.
TotalStain Q is compatible with any instrument capable of green channel excitation (including the Azure Imager & Sapphire Q modules). Combine TotalStain Q with your downstream near-infrared or chemiluminescent imaging protocol for quantitative analysis, without any destaining step required. This two component, room temperature stable fluorescent stain makes total protein normalization both easy and efficient.
Presented by: Dr. Brian Bauereis, Field Application Scientist
At Azure Biosystems we design and manufacture novel lab instrumentation to generate and analyze images of a wide range of assays. Our innovative platforms provide superior performance to a vast array of applications in bioscience. Both platforms were designed to be the most sensitive systems currently available in the market. This talk will explain how Azure has used the latest in technology to achieve such flexibility in chemistry selection and performance from a scientific and technical perspective. This talk will also focus on exciting experimental paradigms using Azure instrumentation. The presenter, Dr. Brian Bauereis, a Yale alumnus, has worked with Azure for 4 years and will present on how these advancements can benefit the scientific and medical communities.
Presented by Dr. Andreas Schrötter, Scientific Sales Manager
Whatever type of imaging your lab does, whether it’s the ubiquitous western blot, fluorescent gels, visualizing gross morphology of tissues or something more unique, The Sapphire™ Biomolecular Imager will deliver outstanding, quantitative detection with NIR and RGB fluorescence, chemiluminescence, and phosphor imaging. While western blotting has been a lab standard for decades, the high performance of our scanner enables also time- and labor-saving extensions of the western blot. One such extension is an cell-based assay in microplates, where plate-grown cells are fixed, permeabilized, and then probed with antibody in situ. The result is an accurate measurement of intracellular protein expression while the cells are still in the plate. In this webinar we present the next generation of laser-based imaging with various types of array based applications such as cell-based assays in microplates and protein microarrays.
Presented by Dr. Andreas Schrötter, Scientific Sales Manager
Multiple imaging modalities give you the freedom to do more. If you’re one of the many scientists that combines biochemical techniques such as gels and Western blots with the visualization of mouse tissue or mouse morphology, it’s time to rethink how much you really need to use a microscope. With resolution down to 10μm, The Sapphire™ Biomolecular Imager enables imaging above the subcellular level and the large scanning bed translates into a ultra-wide field of view compared to a microscope. In this webinar we present the next generation of laser-based imaging with mouse samples like brain, lung, liver and so much more!
Presented by Jan Wolfram, Director, European Sales
What should you consider when starting with a fluorescent Western blotting method? What are differences from the traditional HRP based essays? Is it more quantifiable? Are there any beginners’ mistakes to avoid with the first run? How to make a fluorescent blot a success? These questions will be answered by this introductory webinar from Azure Biosystems.
Presented by Justin Menko, Technical Product Specialist
The use of housekeeping proteins (HKPs) for the normalization of western blot data is the historical norm. With that said, numerous peer reviewed journals and the NIH have started suggesting that researchers transition to using a total protein normalization methodology. This suggestion is based on a few key variables which this webinar addresses. For those not familiar with total protein normalization or wondering if it’s time to transition away from HKPs, this webinar is for you.
Presented by Joy DeTorres, Application Scientist
Western Blotting is a science, but creating beautiful Western blots is an art. Learn tips to improve your Western blotting technique, and troubleshoot common issues with Western blotting and blot imaging to produce clean blots and more quantitative images.
Presented by Dr. Andreas Schrötter, Scientific Sales Manager
Think about it—one instrument, one easy and flexible data acquisition for plant samples. If you’re one of the many scientists that combines biochemical techniques such as gels and Western blots with the visualization of plant samples, it’s time to think about a large field of view scanner. With resolution down to 10μm, The Sapphire™ Biomolecular Imager enables imaging above the subcellular level and the large scanning bed translates into a ultra-wide field of view to allow you to see your plant specimen in full size. In this webinar we present the next generation of laser-based imaging with plant samples like Arabidopsis thaliana roots and seedlings, plant stem cuts, various full size pant leafs and so much more!
Presented by Dr. Stephanie M. Cologna, Assistant Professor, University of Illinois at Chicago
Biomarker research is critical to medical research and the advancement of treatment and drug development. In this webinar, Dr. Stephanie M. Cologna of the University of Illinois at Chicago discusses the differential proteomic and lipidomic signatures of Niemann-Pick Disease, Type C, and how she is combining Western blotting and mass spectrometry in her research.
Presented by Dr. KaiLeng Tan, Azure Biosystems
