Imaging Bacterial colonies

Counting bacterial colonies is a great means to identifying the general bacteria content of a sample. A gelled growth medium, like a Petri dish or plate, contains colonies of bacteria or other microorganisms. The process of imaging bacterial colonies is simple by using gel imagers, such as the Azure Imaging Systems.

An agar plate with E. coli expressing GFPmut3 (green) and mCherry (red). The plate was imaged using red and green LEDs in the Azure 600.
An agar plate with E. coli expressing GFPmut3 (green) and mCherry (red). The plate was imaged using red and green LEDs using the Azure 600 Imager.

What can I use to image bacterial colonies?

The Azure Imaging Systems can be used to image more than just gels and membranes. Because of the depth of field, the imagers can capture images of bacterial or yeast colonies on plates. All of the Azure imagers can capture white light images, but the Azure 400 and 600 can also image fluorophores that fluoresce in the visible spectrum. This allows the capture of microorganisms expressing fluorophores such as GFP and mCherry. This is useful to carrying out on-plate screening for gene insertions or mutations, protein-protein interaction studies, gene transfer studies, characterization of colony morphology, or verification of fluorescent protein expression.

Digital imagers like the 400 can be used for more than just gels and membranes. Other sources of fluorescence on a flat surface can be captured by the imager, including bacterial colonies on a plate. Fluorescent proteins like GFP variants fall in the visible range, meaning the 600 is another option as a digital imager.

Examples of when this might be useful include: on-plate screening for gene insertions or mutations, protein-protein interaction studies, gene transfer studies, characterization of colony morphology, or verification of fluorescent protein expression.

What software is used to count bacterial colonies?

AzureSpot Pro colony counter bacterial plate imaging, data analysis software

AzureSpot Pro software includes a Colony Counter module, making counting of spots or features fast and easy.

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