Western Blotting Workflow

1. PLAN

PLAN YOUR EXPERIMENT AND CHOOSE YOUR DETECTION METHOD

CHEMILUMINESCENT WESTERN BLOTTING

The most common method for Western blot detection is chemiluminescence. Detection by chemiluminescence is relatively sensitive and works well for detecting individual or low abundance proteins.

How it works

In chemiluminescent Western blots, a primary antibody is used to detect your protein of interest, followed by a species-specific secondary antibody to detect the primary antibody. The secondary antibody is conjugated to horseradish peroxidase (HRP), which catalyzes the oxidation of luminol to produce a product that emits light (luminescence). The light given off can be captured by film and developed in a darkroom, or it can be detected by a CCD camera using a digital imager.

FLUORESCENT WESTERN BLOTTING

Fluorescence Western blotting is beneficial when your experiment requires quantitation, particularly between different proteins in the same sample. Fluorescent methods are especially useful for detection of post-translationally modified versions of proteins, since the difference in molecular weight is often not enough for clear resolution on an average gel.

How it works

In fluorescent Western blots, the secondary antibody is conjugated to a dye molecule that can undergo fluorescence—meaning, it will give off light of a specific wavelength when excited with light of a shorter wavelength. Fluorophores are available in the visible or infrared (IR) spectrum. Because different fluorophores can give off light in different ranges, fluorescent Western blotting can be used to multiplex—meaning, different target proteins can be visualized on the same blot at the same time.

With fluorescence, you have the option of using secondary antibodies conjugated to fluorophores, or you can label your primary antibodies with fluorophores of your choice to perform direct Westerns.

CHEMILUMINESCENT OR FLUORESCENT WESTERN BLOTTING?

COMMON APPLICATIONS

Chemiluminescence

Checking protein purification
Presence or absence of one protein in a lysate
Detecting low abundance proteins

Fluorescence

Analyzing multiple proteins
Posttranslational modifications
Quantitative comparisons

2. SEPARATE

RESOLVE YOUR PROTEINS BY ELECTROPHORESIS AND TRANSFER TO A MEMBRANE

Is Western blotting required?

Some applications don’t require western blotting and can be done by directly staining proteins in the gel after separation.

Applications

Checking for a single band after protein purification.

AzureRed Fluorescent Protein Stain (AC2124) qualitatively stains gels for fluorescent imaging systems.

Choosing your membrane

The membrane provides a material that binds proteins as they move out of the gel matrix. The two common materials are nitrocellulose and PVDF.

Nitrocellulose (AC2106–AC2107)

For low molecular weight proteins
Typically used for chemi-applications
Not ideal for stripping

PVDF (AC2105, AC2108–AC2109)

High binding capacity is great for low-expression proteins
Typically used for fluorescence (less background autofluorescence)
High protein retention for stripping and reprobing

Setting up a good transfer

Proper transfer set-up involves a “sandwich” where gel and membrane are laid in direct contact between blotting paper, flanked by electrode plates. The transfer buffer allows current to flow between electrodes to carry proteins from gel to membrane.

Blotting paper (filter paper) provides consistent pressure so no gaps appear between membrane and gel while remaining absorbent enough for transfer buffer to penetrate the layers.

Azure Pre-cut Blotting Paper (AC2110–AC2111) adds efficiency to this sandwich set-up.

Azure Transfer Buffer (AC2127) is a proprietary formula designed for quick, high quality wet transfer and sensitive western blotting.

Before blotting

Verifying that total protein was sufficiently transferred from gel to the membrane is important when optimizing a transfer setup, condition, or experiment.

Ponceau staining can be used to visualize all protein on the membrane. After, the membrane can be destained for immediate western blotting.

Azure Ponceau Stain (AC2125) can ensure efficient transfer of proteins onto nitrocellulose or PVDF membranes.

Azure Opaque Incubation Trays (AC2120–AC2123) offer protection for light-sensitive membrane incubation. The surface is smoothed to prevent scratches and damage to membranes.

Azure Clear Incubation Trays (AC2150–AC253) allow protection of your blots, with a smoothed surface to prevent membrane damage and transparency for easy monitoring.

3. PROBE

BLOCK MEMBRANE AND PROBE FOR PROTEINS OF INTEREST WITH OPTIMIZED ANTIBODIES

Blocking the membrane

Blocking is important to prevent nonspecific background signals from interfering with your data.

Protein Free Blocking Buffer (AC2112) works best for purified proteins & antibodies with high cross reactivity with protein based blockers.

Fluorescent Blocking Buffer (AC2190) works best for cell lysates and low quality primary antibodies that require protein blockers.

Chemi Blot Blocking Buffer (AC2148) works best for chemiluminescent Western blots.

Need help getting started with fluorescent Western blotting? Check out the demo kit, page 8.

AzureSpectra Demo Kit (AC2172–AC2179)

Probe with antibody

Make sure concentrations for new antibodies are optimized.

Have a validated primary antibody? Try a direct Western by labeling your primary antibody with fluorescent dye.

AzureSpectra Labeling Kit (AC2185–AC2189) allows quick and easy labeling of primary antibodies–see more on page 7.

NEED TO REPROBE?

HRP Stripping buffer (AC2154) efficiently removes antibodies from your blot.

Scan for more information about our free substrate samples

Washing after incubations

It is essential to wash excess antibody off the membrane after each incubation to prevent background signal. For fluorescent blots, make sure to rinse off excess wash buffer containing detergent, since it can fluoresce.

Blot Washing Buffer (AC2113) works well for chemiluminescent HRP substrates and visible fluorescent blots.

Fluorescent Blot Washing Buffer (AC2145) works best with NIR fluorescent blots.

Azure HRP secondary antibodies (AC2114- AC2119, AC2149) offer a range of species specificities.

Fluorescent secondary antibodies (AC2128- AC2139, AC2156-71) come in visible and near IR fluorophores.

Chemiluminescence detection

Choose your HRP substrate:

Radiance (AC2101) produces a strong, long-lasting signal with extremely low background.

Radiance Plus (AC2103) is the most sensitive HRP substrate and allows you to detect bands not visualized with other substrates.

ChemiWriter ECL (AC2146) adsorbs to nitrocellulose and PVDF membranes, and reacts with HRP substrates to produce chemiluminescence.

Blot Development Folders (AC2126) protect your blot during imaging and keep it wet for longer lasting signal.

Fluorescence detection

Background Quenching Sheets (AC2144, AC2147) reduce background autofluorescence when imaging your fluorescent (or chemi) blot.

4. VISUALIZE

DETECT YOUR PROTEINS OF INTEREST BY CHEMILUMINESCENCE OR FLUORESCENCE

AZURE IMAGING SYSTEMS

The Azure Imaging Systems offers 6 unique Western blot imaging systems. Select which one fits your applications now, and learn more about upgrading later when your needs change.

Upgrades available for chemiluminescence, RGB fluorescence and NIR fluorescence.

Q Module—Upgrade the Azure 500 and Azure 300 to include a green fluorescence Q module to add a dedicated channel for total protein normalization in quantitative Western blots.

5. ANALYZE

ANALYZE YOUR DATA WITH EASY-TO-USE SOFTWARE TO GET THE ANSWERS YOU NEED

AZURESPOT PRO ANALYSIS

AzureSpot Pro helps you interpret your data easily and accurately. Analyze your gels and blots in either manual or automatic modes.

Automatically detect bands, even on distorted gels, and apply your selected method of background correction.

Visualize intensity plots by lane (sample) and channel (probe) to get detailed information about your proteins of interest.

The integrated tutorial helps you get the most information out of your analyses. Generate a report of your experiment or export your data to other applications (e.g. Excel) for further analysis.

Product features

Integrated tutorial
Report generator
Automatic saturated pixel detection
Analyze multiple blots or plates per image
Lane creation tools
Molecular size calibration
Quantity calibration tool

Blot and array normalization tool
Module for easy shape selection/deselection
Wide range of data fields to display in measurements
Annotation tools for comments and highlighting of image

AzureSpot Pro can

Automatically detect bands
Correct for background
Calculate molecular weights
Quantify fluorescent signals
Annotate your results
Visualize multiplex analyses
Count colonies
Analyze grids, well-plates and arrays
Export reports

LABELING KITS

AZURESPECTRA LABELING KITS

Label your primary or secondary antibody with a fluorescent dye

Fast and simple antibody labeling

Easy and fast—The fluorescent antibody labeling kit provides the materials necessary to fluorescently label the antibody of your choice in less than 1 hour
Label primary antibodies—use antibodies that your lab has already validated and optimized. Labeled primary antibodies can then be used for direct Westerns.
Label secondary antibodies—secondary antibodies can be labeled for traditional indirect Western blot applications
Choose from 5 dyes—the non-overlapping emission spectra of the fluorophores available for labeling means you can easily perform multiplexing

Perform multiplexing assays quick and easily. Fluorescent Western blot imaged with Azure c600. Secondary antibodies were labeled with the AzureSpectra labeling kit: anti-rabbit with AzureSpectra 700, anti-mouse with AzureSpectra 550, and anti-rat with AzureSpectra 800. Primary antibodies used were rat anti-tubulin, rabbit anti-beta actin, and mouse anti-GAPDH.

Simple labeling protocol.

Each kit includes

Sufficient reagents for labeling of 2 x 50 μg of antibody

Labeling Buffer
Dye solution
Quenching solution
Neutralization buffer
Two G-25 columns

Dye	Excitation max. (nm)	Emission max. (nm)
Dye-490	491	515
Dye-550	551	565
Dye-650	653	672
Dye-IR7000	692	709
Dye-IR800	783	800

DEMO KITS

AZURESPECTRA DEMO KIT

New to fluorescent Western blotting?

LET US HELP YOU GET STARTED

Azure’s Fluorescent Western blotting Demo Kit contains everything you need to perform single color fluorescent Westerns using your samples and primary antibodies.

What you need

Your protein sample, resolved by SDS PAGE
Primary antibody against protein of interest

How to use

Run a gel of your sample and transfer to the provided PVDF membrane
Block using Fluorescent Blot Blocking Buffer
Probe using your chosen primary antibody
Wash with Fluorescent Blot Wash Buffer
Incubate with the provided secondary antibody
Wash again with Fluorescent Blot Wash Buffer
Rinse with PBS
Take an image of your fluorescent blot using the provided background quenching sheets

Blots probed with AzureSpectra secondary antibodies and imaged using NIR with an Azure 600. Left: probed with AzureSpectra 800 secondary (green). Right: probed with AzureSpectra 700 (red).

Each kit contains

Enough material for 1 Western blot

9x7cm Low Fluorescence PVDF membrane
10x Fluorescent Blot Washing Buffer, 30mL
1x Fluorescent Blot Blocking Buffer, 30mL
Secondary Antibody, 4μL

PRODUCTS

ORDERING INFORMATION

Protein Gel Electrophoresis

AC2124

AzureRed Fluorescent Protein Stain—fluorescent total protein stain for 1D and 2D gels, sufficient for 20 minigels, 5 ml

Transfer

AC2105

Low Fluorescence Western Membrane (PVDF)–0.45 μm—precut PVDF membrane, 9x7cm vertical gels, 10 sheets

AC2106

Nitrocellulose Transfer Membrane–0.45 μm—pre-cut membrane, 8×10 cm mini vertical gels, ideal for larger proteins, 10 sheets

AC2107

Nitrocellulose Transfer Membrane–0.22 μm—pre-cut membrane, 8×10 cm mini vertical gels, ideal for smaller proteins, 10 sheets

AC2108

Low Fluorescence Western Membrane (PVDF)–0.45 μm—pre-cut PVDF membrane, 10×15 cm vertical gels, 10 sheets

AC2109

Low Fluorescence Western Membrane (PVDF)–0.45 μm—pre-cut PVDF membrane, 13×18 cm vertical gels, 10 sheets

AC2110

Blotting Paper for Western Blotting—pre-cut blotting paper for Western blot transfer, 7×9 cm vertical gels, 60 sheets

AC2127

Azure Transfer Buffer—50x concentrated solution sufficient for 50–100 blots

AC2125

Azure Ponceau Stain—visible reversible total protein stain for proteins transferred to membrane, sufficient for staining 50 blots 9x7cm, 500 ml

Blocking

AC2112

Azure Protein Free Blot Blocking Buffer—protein-free blocking solution (5x concentrate) for immunoblots, 200 ml

AC2190

Azure Fluorescent Blot Blocking Buffer—blocking solution (1X) for fluorescent immunoblots, 500 ml

AC2148

Azure Chemi Blot Blocking Buffer—blocking solution (1X) for chemiluminescent immunoblots, 500 ml

Washing

AC2113

Azure Blot Washing Buffer—immunoblot washing solution 10x concentrate, 500ml

AC2145

Azure Fluorescent Blot Washing Buffer—immunoblot washing solution 10x concentrate, for use with fluorescent secondaries, 500 ml

Scan me QR code

Blot Accessories

AC2126

Blot Development Folder—transparent plastic sheets to hold blots for imaging (alternative to plastic wrap), 100 pcs

AC2144

Quenching Sheets, small—10 background quenching sheets, for use with fluorescent or chemiluminescent samples (9×12.5 cm)

AC2147

Quenching Sheets, large—10 background quenching sheets, for use with fluorescent or chemiluminescent samples (16×22 cm)

Detection Substrates

AC2101

Radiance—HRP Substrate for CCD 150 ml kit, contains substrate for 1500cm² membrane

AC2103

Radiance Plus—femtogram HRP Substrate 150 ml kit, contains substrate for 1500cm² membrane

AC2146

Azure ChemiWriter ECL—a chemiluminescent marker that enables you to write or draw on your transfer membranes, resulting in a chemiluminescent signal on your markings once substrate is added

Kits

AC2140

AzureSpectra rb700/ms800 Western Kit, with Protein Free Block—kit contains materials for 10 Western Blots: PVDF membranes, wash buffer, protein free blocking buffer, secondary antibodies AzureSpectra rb700/ms800

AC2141

AzureSpectra ms700/rb800 Western Kit, with Protein Free Block—kit contains materials for 10 Western Blots: PVDF membranes, wash buffer, protein free blocking buffer, secondary antibodies AzureSpectra ms700/rb800

AC2104

AzureSpectra rb650/ms550 Western Kit, with Protein Free Block—kit contains materials for 10 Western Blots: PVDF membranes, wash buffer, protein free blocking buffer, secondary antibodies rb650/ms550

AC2191

AzureSpectra rb700/ms800 Western Kit, with Fluorescent Block—kit contains materials for 10 Western Blots: PVDF membranes, wash buffer, fluorescent blocking buffer, secondary antibodies AzureSpectra rb700/ms800

AC2192

AzureSpectra ms700/rb800 Western Kit, with Fluorescent Block—kit contains materials for 10 Western Blots: PVDF membranes, wash buffer, fluorescent blocking buffer, secondary antibodies AzureSpectra ms700/rb800

AC2193

AzureSpectra rb650/ms550 Western Kit, with Fluorescent Block—kit contains materials for 10 Western Blots: PVDF membranes, wash buffer, fluorescent blocking buffer, secondary antibodies AzureSpectra rb650/ms550

Stripping Buffer

AC2154

HRP Stripping Buffer—uniformly removes antibodies from developed HRP Western blots so the blot can be reprobed with another set of antibodies

Incubation Trays

AC2120

7×5 cm, opaque black top and bottom—for staining and washing light sensitive gels and membranes, includes 5 trays

AC2121

9×6 cm, opaque black top and bottom—for staining and washing light sensitive gels and membranes, includes 4 trays

AC2122

11×9 cm, opaque black top and bottom—for staining and washing light sensitive gels and membranes, includes 3 trays

AC2123

15.5×10.5 cm, opaque black top and bottom—for staining and washing light sensitive gels and membranes, includes 2 trays

AC2150

7×5 cm, clear—for staining and washing gels and membranes, includes 5 trays

AC2151

9×6 cm, clear—for staining and washing gels and membranes, includes 4 trays

AC2152

11×9 cm, clear—for staining and washing gels and membranes, includes 3 trays

AC2153

15.5×10.5 cm, clear—for staining and washing gels and membranes, includes 2 trays

AzureSpectra 800 secondary antibodies

AC2134

Goat-anti-rabbit—AzureSpectra 800 conjugated secondary antibody for fluorescent Westerns, 250 μl

AC2135

Goat-anti-mouse—AzureSpectra 800 conjugated secondary antibody for fluorescent Westerns, 250 μl

AC2136

Goat-anti-human—AzureSpectra 800 conjugated secondary antibody for fluorescent Westerns, 250 μl

AC2137

Goat-anti-chicken—AzureSpectra 800 conjugated secondary antibody for fluorescent Westerns, 250 μl

AC2138

Goat-anti-rat—AzureSpectra 800 conjugated secondary antibody for fluorescent Westerns, 250 μl

AC2139

Goat-anti-guinea pig—AzureSpectra 800 conjugated secondary antibody for fluorescent Westerns, 250 μl

AC2157

Donkey-anti-goat—AzureSpectra 800 conjugated secondary antibody for fluorescent Westerns, 250 μl

AzureSpectra 700 secondary antibodies

AC2128

Goat-anti-rabbit—AzureSpectra 700 conjugated secondary antibody for fluorescent Westerns, 250 μl

AC2129

Goat-anti-mouse—AzureSpectra 700 conjugated secondary antibody for fluorescent Westerns, 250 μl

AC2130

Goat-anti-human—AzureSpectra 700 conjugated secondary antibody for fluorescent Westerns, 250 μl

AC2131

Goat-anti-chicken—AzureSpectra 700 conjugated secondary antibody for fluorescent Westerns, 250 μl

AC2132

Goat-anti-rat—AzureSpectra 700 conjugated secondary antibody for fluorescent Westerns, 250 μl

AC2132

Goat-anti-rat—AzureSpectra 700 conjugated secondary antibody for fluorescent Westerns, 250 μl

AC2133

Goat-anti-guinea pig—AzureSpectra 700 conjugated secondary antibody for fluorescent Westerns, 250 μl

AC2156

Donkey-anti-goat—AzureSpectra 700 conjugated secondary antibody for fluorescent Westerns, 250 μl

AzureSpectra 650 secondary antibodies

AC2165

Goat-anti-rabbit—AzureSpectra 650 conjugated secondary antibody for fluorescent Westerns, 250 μl

AC2166

Goat-anti-mouse—AzureSpectra 650 conjugated secondary antibody for fluorescent Westerns, 250 μl

AC2167

Goat-anti-human—AzureSpectra 650 conjugated secondary antibody for fluorescent Westerns, 250 μl

AC2168

Goat-anti-chicken—AzureSpectra 650 conjugated secondary antibody for fluorescent Westerns, 250 μl

AC2169

Goat-anti-rat—AzureSpectra 650 conjugated secondary antibody for fluorescent Westerns, 250 μl

AC2170

Goat-anti-guinea pig—AzureSpectra 650 conjugated secondary antibody for fluorescent Westerns, 250 μl

AC2171

Donkey-anti-goat—AzureSpectra 650 conjugated secondary antibody for fluorescent Westerns, 250 μl

AzureSpectra 550 secondary antibodies

AC2158

Goat-anti-rabbit—AzureSpectra 550 conjugated secondary antibody for fluorescent Westerns, 250 μl

AC2159

Goat-anti-mouse—AzureSpectra 550 conjugated secondary antibody for fluorescent Westerns, 250 μl

AC2160

Goat-anti-human—AzureSpectra 550 conjugated secondary antibody for fluorescent Westerns, 250 μl

AC2161

Goat-anti-chicken—AzureSpectra 550 conjugated secondary antibody for fluorescent Westerns, 250 μl

AC2162

Goat-anti-rat—AzureSpectra 550 conjugated secondary antibody for fluorescent Westerns, 250 μl

AC2163

Goat-anti-guinea pig—AzureSpectra 550 conjugated secondary antibody for fluorescent Westerns, 250 μl

AC2164

Donkey-anti-goat—AzureSpectra 550 conjugated secondary antibody for fluorescent Westerns, 250 μl

HRP secondary antibodies

AC2114

Goat-anti-rabbit—HRP conjugated secondary antibody for chemi Westerns, 500 μl

AC2115

Goat-anti-mouse—HRP conjugated secondary antibody for chemi Westerns, 500 μl

AC2116

Goat-anti-human—HRP conjugated secondary antibody for chemi Westerns, 500 μl

AC2117

Goat-anti-chicken—HRP conjugated secondary antibody for chemi Westerns, 500 μl

AC2118

Goat-anti-rat—HRP conjugated secondary antibody for chemi Westerns, 500 μl

AC2119

Goat-anti-guinea pig—HRP conjugated secondary antibody for chemi Westerns, 500 μl

AC2149

Donkey-anti-goat—HRP conjugated secondary antibody for chemi Westerns, 500 μl

AzureSpectra Demo Kits

AC2172

Goat-anti-mouse 700—kit contains 1 PVDF membrane, blocking buffer, wash buffer, goat anti-mouse 700 secondary antibody

AC2173

Goat-anti-rabbit 700—kit contains 1 PVDF membrane, blocking buffer, wash buffer, goat anti-rabbit 700 secondary antibody

AC2174

Goat-anti-mouse 800—kit contains 1 PVDF membrane, blocking buffer, wash buffer, goat anti-mouse 800 secondary antibody

AC2175

Goat-anti-rabbit 800—kit contains 1 PVDF membrane, blocking buffer, wash buffer, goat anti-rabbit 800 secondary antibody

AC2176

Goat-anti-mouse 650—kit contains 1 PVDF membrane, blocking buffer, wash buffer, goat anti-mouse 650 secondary antibody

AC2177

Goat-anti-rabbit 650—kit contains 1 PVDF membrane, blocking buffer, wash buffer, goat anti-rabbit 650 secondary antibody

AC2178

Goat-anti-mouse 550—kit contains 1 PVDF membrane, blocking buffer, wash buffer, goat anti-mouse 550 secondary antibody

AC2179

Goat-anti-rabbit 550—kit contains 1 PVDF membrane, blocking buffer, wash buffer, goat anti-rabbit 550 secondary antibody

AzureSpectra Labeling Kits

AC2185

For Westerns – 490—for covalently labeling antibodies with a fluorescent dye. Includes labeling buffer, dye solution, quenching solution, neutralization buffer and two G-25 columns. Each kit includes sufficient reagents to perform two labellings of 50 micrograms of antibody.

AC2186

For Westerns – 550—for covalently labeling antibodies with a fluorescent dye. Includes labeling buffer, dye solution, quenching solution, neutralization buffer and two G-25 columns. Each kit includes sufficient reagents to perform two labellings of 50 micrograms of antibody.

AC2187

For Westerns – 650—for covalently labeling antibodies with a fluorescent dye. Includes labeling buffer, dye solution, quenching solution, neutralization buffer and two G-25 columns. Each kit includes sufficient reagents to perform two labellings of 50 micrograms of antibody.

AC2188

For Westerns – 700—for covalently labeling antibodies with a fluorescent dye. Includes labeling buffer, dye solution, quenching solution, neutralization buffer and two G-25 columns. Each kit includes sufficient reagents to perform two labellings of 50 micrograms of antibody.

AC2189

For Westerns – 800—for covalently labeling antibodies with a fluorescent dye. Includes labeling buffer, dye solution, quenching solution, neutralization buffer and two G-25 columns. Each kit includes sufficient reagents to perform two labellings of 50 micrograms of antibody.

Azure Imaging Systems

AZI600-01

Azure Imager 600

AZI500-01

Azure Imager 500

AZI400-01

Azure Imager 400

AZI300-01

Azure Imager 300

AZI280-01

Azure Imager 280

AZI200-01

Azure Imager 200

CS1000

chemiSOLO Imager

Azure Imager 600 in use

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gaconnector_lc_referrer	6 months	This cookie is set to determine the last click referrer.
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gaconnector_lc_term	6 months	This cookie is set to determine the last click term.
gaconnector_longitude	6 months	This cookie is set to determine the longitude.
gaconnector_OS	6 months	This cookie is set to determine the operating system.
gaconnector_region	6 months	This cookie is set to determine the region.
gaconnector_time_passed	6 months	This cookie stores data on the time spent on the website and its sub-pages, during the current session.
gaconnector_time_zone	6 months	This cookie is set to determine the time zone.
gaconnector2_id	never	This cookie is used by the GA Connector service to gather information regarding visitor activity and related advertising campaigns on the website upon enquiry.
MR	7 days	This cookie, set by Bing, is used to collect user information for analytics purposes.
sbjs_current	session	Sourcebuster sets this cookie to identify the source of a visit and stores user action information in cookies. This analytical and behavioural cookie is used to enhance the visitor experience on the website.
sbjs_current_add	session	Sourcebuster sets this cookie to identify the source of a visit and stores user action information in cookies. This analytical and behavioural cookie is used to enhance the visitor experience on the website.
sbjs_first	session	Sourcebuster sets this cookie to identify the source of a visit and stores user action information in cookies. This analytical and behavioural cookie is used to enhance the visitor experience on the website.
sbjs_first_add	session	Sourcebuster sets this cookie to identify the source of a visit and stores user action information in cookies. This analytical and behavioural cookie is used to enhance the visitor experience on the website.
sbjs_migrations	session	Sourcebuster sets this cookie to identify the source of a visit and stores user action information in cookies. This analytical and behavioural cookie is used to enhance the visitor experience on the website.
sbjs_session	1 hour	Sourcebuster sets this cookie to identify the source of a visit and stores user action information in cookies. This analytical and behavioural cookie is used to enhance the visitor experience on the website.
sbjs_udata	session	Sourcebuster sets this cookie to identify the source of a visit and stores user action information in cookies. This analytical and behavioural cookie is used to enhance the visitor experience on the website.
siqlsdb	never	Zoho sets this cookie to generate a unique ID for the session. This allows the website to obtain data on visitor behaviour for statistical purposes.
utsdb	never	Zoho SalesIQ sets this cookie to register data on visitor's website behaviour.
zabUserId	1 year	Zoho PageSense sets this cookie to store a unique ID for every user who visits that website.
zpssr*	session	Zoho PageSense sets this cookie to identify the top-level domain during a session, ensuring accurate tracking and analysis of user interactions across different domains.
zsc*	1 hour	Zoho sets this cookie to uniquely identify the current session. This enables comprehensive tracking of user interactions for detailed analytics and website performance optimization.

Cookie	Duration	Description
ANONCHK	10 minutes	The ANONCHK cookie, set by Bing, is used to store a user's session ID and also verify the clicks from ads on the Bing search engine. The cookie helps in reporting and personalization as well.
bcookie	1 year	LinkedIn sets this cookie from LinkedIn share buttons and ad tags to recognize browser IDs.
cb_user_id	1 year	Clearbit sets this cookie to collect data on visitors. This information is used to assign visitors into segments, making website advertising more relevant.
IDE	1 year 24 days	Google DoubleClick IDE cookies are used to store information about how the user uses the website to present them with relevant ads and according to the user profile.
MUID	1 year 24 days	Bing sets this cookie to recognize unique web browsers visiting Microsoft sites. This cookie is used for advertising, site analytics, and other operations.
NID	6 months	NID cookie, set by Google, is used for advertising purposes; to limit the number of times the user sees an ad, to mute unwanted ads, and to measure the effectiveness of ads.
test_cookie	15 minutes	The test_cookie is set by doubleclick.net and is used to determine if the user's browser supports cookies.
VISITOR_INFO1_LIVE	5 months 27 days	A cookie set by YouTube to measure bandwidth that determines whether the user gets the new or old player interface.
YSC	session	YSC cookie is set by Youtube and is used to track the views of embedded videos on Youtube pages.
yt-remote-connected-devices	never	YouTube sets this cookie to store the video preferences of the user using embedded YouTube video.
yt-remote-device-id	never	YouTube sets this cookie to store the video preferences of the user using embedded YouTube video.
yt.innertube::nextId	never	This cookie, set by YouTube, registers a unique ID to store data on what videos from YouTube the user has seen.
yt.innertube::requests	never	This cookie, set by YouTube, registers a unique ID to store data on what videos from YouTube the user has seen.

Cookie	Duration	Description
__rafm	session	No description available.
__rasesh	2 years 9 months 23 days	No description available.
__Secure-ROLLOUT_TOKEN	6 months	Description is currently not available.
__Secure-YEC	past	Description is currently not available.
__Secure-YNID	6 months	Description is currently not available.
__tld__	session	Description is currently not available.
_bioz_depot_session	session	Description is currently not available.
_depot_session	session	No description
_vh_gid	7976 years 10 months 22 days 20 hours 2 minutes	No description
_vh_sid	15 minutes	No description
_vh_sid_pn	session	No description
383aeadb58	session	No description available.
53d772c94a	session	Description is currently not available.
663a60c55d	session	No description available.
98bac1f50f	session	No description available.
ac09458e72	session	No description
azurebiosystems-_zldp	1 year 1 month 4 days	Description is currently not available.
azurebiosystems-_zldt	1 day	Description is currently not available.
c72887300d	session	No description available.
cbtest	1 year	Description is currently not available.
COMPASS	1 hour	No description
crmcsr	session	No description available.
debug	never	No description available.
DEVICE_INFO	5 months 27 days	No description
drscc	session	Description is currently not available.
e188bc05fe	session	No description available.
fd6b13af5c	session	No description available.
gaconnector_fc_timestamp	6 months	Description is currently not available.
gaconnector_GA_Measurement_ID	6 months	Description is currently not available.
gaconnector_GA_Session_ID	6 months	Description is currently not available.
gaconnector_lc_timestamp	6 months	Description is currently not available.
gclid	1 month	No description
iamcsr	session	No description available.
MSPTC	1 year 24 days	Description is currently not available.
ncbi_sid	1 year	No description available.
nevercache-b39818	session	Description is currently not available.
pfjscookies	1 year	Description is currently not available.
ps_payloadSeqId	2 hours	No description available.
sales_6339725afa6243a997dc48e2eac3fe89_1722267915719zabu0.7622164321864138	1 year	Description is currently not available.
sales_6339725afa6243a997dc48e2eac3fe89_1722267916062zabu0.34545636830562443	1 year	Description is currently not available.