Azure BiosystemsHigh-quality and affordable reagents, imagers, and qPCR systems
Azure Biosystems
Smart protocols, high-quality data, and robust durability
Engineered for a wide variety of qPCR applications with high sensitivity and wide dynamic range
Our user interface allows simple touch screen assay setup. Saved protocols make assay set up easy and reproducible
The Cielo’s optics and thermal block are designed to deliver the same uniformity and reproducibility over at least 1000 qPCR experiments, giving you confidence in the reliability of the instrument
Easily operates without use of external. Allows for transfer data via Ethernet, Wi-Fi, or USB
 |  | |
|---|---|---|
| Azure Cielo 3 | Azure Cielo 6 | |
| SAMPLE CAPACITY (WELLS) | 96 | 96 |
| COMPATIBLE PLATES/TUBES | 96-WELL PLATES & TUBES AND 8 OR 12-WELL STRIPS & SINGLE TUBES | 96-WELL PLATES & TUBES AND 8 OR 12-WELL STRIPS & SINGLE TUBES |
| REACTION VOLUME | 5–150uL | 5–150uL |
| EXCITATION SOURCE | LED, EXCITATION RANGE: 450–580NM AND 610–700NM | LED, EXCITATION RANGE: 450–730NM |
| DETECTION CHANNELS | 3 | 6 |
| MULTIPLEXING | UP TO 3 TARGETS | UP TO 6 TARGETS |
| REACTION TEMPERATURE | 4°C TO 99.9°C | 4°C TO 99.9°C |
| MAX. BLOCK RAMP RATE | 6°C/SEC | 6°C/SEC |
| AVG. SAMPLE RAMP RATE | 4°C/SEC | 4°C/SEC |
| TEMPERATURE UNIFORMITY | ±0.2°C | ±0.2°C |
| TEMPERATURE ACCURACY | ±0.1°C | ±0.1°C |
| CUSTOM DYE/CHEMISTRY |  | |
| ONBOARD MEMORY | 32GB EQUIVALENT TO 20,000 EXPERIMENTS | 32GB EQUIVALENT TO 20,000 EXPERIMENTS |
| CONNECTIVITY | USB, WI-FI, ETHERNET | USB, WI-FI, ETHERNET |
| FOOTPRINT (W/D/H) | 12" X 20" X 17" (30.5 X 50.8 X 43.2 CM) | 12" X 20" X 17" (30.5 X 50.8 X 43.2 CM) |
| | |||
|---|---|---|---|---|
| Dyes/Probes | Azure Cielo 3 | Azure Cielo 6 | Filter wavelength (nm) Excitation | Filter wavelength (nm) Emission |
| SYBR™ Green, EvaGreen™, FAM™ | | | 475 ± 14 | 524 ± 12 |
| VIC®, HEX™, JOE™, CAL Fluor® 540, CAL Fluor®, Orange 560 | | | 527 ± 10 | 565 ± 12 |
| TAMRA™, Cy3 | | 572 ± 7.5 | 623 ± 12 | |
| ROX™, TEX®615, CAL Fluor® Red 610 | | 537 ± 13 | 583 ± 11 | |
| CY®5, Quasar®670, Liz®, Mustang Purple® | | | 623 ± 12 | 676 ± 18.5 |
| CY® 5.5, Quasar 705 | | 655 ± 7.5 | 711 ± 12.5 |
Other dyes/probes are possible depending on the system configuration.
Each well is color coded by sample type, biological replicate, and technical replicate. Well type and standard curve values are also easily visible.
NIR lasers keep signal high and background low. Our high-performance multiplex NIR lasers and filters deliver robust excitation energy which maximizes emission strength for maximum sensitivity.
This feature makes it easy to review your samples and quickly understand your data.
The Cielo’s efficient well-based scanning technology scans multiple data points within each well, eliminating the need for normalization dyes such as ROX.
Amplification curves generated using human genomic DNA template along with human GAPDH primers and a no ROX SYBR Green qPCR mix.
Competitive systems only acquire 1 pixel/well compared to the Cielo’s ability to capture approximately 100,000 pixels/wells, enabling accurate, reproducible and sensitive representation of fluorescent intensities from each qPCR well.
High-quality engineering includes Peltier-based thermal block system designed for superior thermal cycling and unmatched optical design to ensure every run is accurate and reproducible.
Unparalleled uniformity. Amplification curves for 96 replicates shown on a linear plot
(A) and a logarithmic plot (Figure B). In each well, 105 copies of GAPDH template were amplified in the presence of GAPDH primers and BioRad™ Sso Advanced SYBR Green Mix®. Average Cq = 19.1, Coefficient of variance (Cv)= 0.002.
Comfortably use up to 6 fluorescent channels, which allow for flexible dye usage and broad application. Because Rox is not required, it can also be used as a probe.
The Azure Cielo Dx qPCR system for IVD use brings you the accuracy and sensitivity you need for your research, with intuitive touch screen software. With 6-target detection, the flexibility you need is at your fingertips.
USA: For IVD use. The Azure Cielo 3 Dx and Azure Cielo 6 Dx Real-time PCR systems are registered with the U.S. FDA as Class II 510(k) exempt devices.
Europe: For IVD use. The Azure Cielo 3 Dx and Azure Cielo 6 Dx systems are CE marked under the In Vitro Diagnostic Medical Devices Directive (98/79/EC). The CE IVD–authorized Azure Cielo Dx Systems are for distribution and use the following countries: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czechia, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Liechtenstein, Lithuania, Luxembourg, Malta, the Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, and the United Kingdom.
As a lab that has been carrying out high volume PCR testing for the last 12 months, the Azure Cielo Real-Time PCR machine has been a fantastic addition to our suite of equipment to facilitate this process. The comparison data we have achieved… more
It is a very user friendly instrument. A very rapid thermocycler, with very good reproducibility. A good instrument to scale and increase produtivity. We have 3 in our lab and I really recomend this real time PCR system for diferent aplications… more
| Document Type | Description | |
|---|---|---|
| Brochure | Cielo Brochure | DOWNLOAD |
| Application Note | Using the Azure Cielo™ Real-Time PCR System for SARS-CoV-2 viral RNA detection | DOWNLOAD |
| Application Note | 6-Channel Multiplex qPCR Using Azure Cielo Real-Time PCR System | DOWNLOAD |
| Application Note | Sensitive and Accurate Single-Copy Amplicon Detection in Azure Cielo Real-Time PCR System | DOWNLOAD |
| Application Note | Azure Cielo™ Real-Time PCR Optical Design Eliminates the Need for Passive Reference Dye | DOWNLOAD |
| Application Note | What Sets the Cielo Apart | DOWNLOAD |
| Application Note | Detection of low copy telomeric genes with Cielo | DOWNLOAD |
| Application Note | GoTaq Enviro Wastewater SARS-CoV-2 | DOWNLOAD |
| Application Note | qPCR Troubleshooting Guide | DOWNLOAD |
| Technical Note | SNP Genotyping/Allelic Discrimination | DOWNLOAD |
| Technical Note | Adding Dyes and Color Compensation | DOWNLOAD |
Azure Biosystems Live Webinar Series How Protein Acetylation Modulates Immune Response Against Melanoma
Azure Cielo is for research-use only, and not for use in diagnostic procedures. Contact us today for a quote and to learn more about how Azure is changing how researchers qPCR.
| Part Number | Product Name | |
|---|---|---|
| AIQ060 | Azure Cielo 6 qPCR | REQUEST A QUOTE |
| AIQ030 | Azure Cielo 3 qPCR | REQUEST A QUOTE |
| AIQ315 | Azure Cielo 6 Dx qPCR (for In-vitro Diagnostics) | REQUEST A QUOTE |
| AIQ314 | Azure Cielo 3 Dx qPCR (for In-vitro Diagnostics) | REQUEST A QUOTE |
