Azure Cielo™ Real-time PCR system

Smart protocols, high-quality data, and robust durability

Azure Cielo Benefits



Engineered for a wide variety of qPCR applications with high sensitivity and wide dynamic range.


Intelligent Workflow

Our user interface allows simple touchscreen assay setup. Saved protocols make assay set up easy and reproducible.


Reliable and Robust

Optics and thermal block are designed to deliver the same uniformity and reproducibility over at least 1000 qPCR experiments.



Easily operates without use of external. Allows for transfer data via Ethernet, Wi-Fi, or USB.

Compare Models

Compare Models (Dyes/Probes)

Other dyes/probes are possible depending on the system configuration.

Key Features

Quickly Set up and Confirm Plate Layout

Each well is color coded by sample type, biological replicate, and technical replicate. Well type and standard curve values are also easily visible.

View Each Individual Amplification Trace by Target or Sample

NIR lasers keep signal high and background low. Our high-performance multiplex NIR lasers and filters deliver robust excitation energy which maximizes emission strength for maximum sensitivity. 

2D "Heat Map" of Samples

This feature makes it easy to review your samples and quickly understand your data.

Optical Design Eliminates the Need for ROX

The Cielo’s efficient well-based scanning technology scans multiple data points within each well, eliminating the need for normalization dyes such as ROX.

Amplification curves generated using human genomic DNA template along with human GAPDH primers and a no ROX SYBR Green qPCR mix.

Approximately 100,000 Data Points/Wells Using Total Well Detection Technology

Competitive systems only acquire 1 pixel/well compared to the Cielo’s ability to capture approximately 100,000 pixels/wells, enabling accurate, reproducible and sensitive representation of fluorescent intensities from each qPCR well.

Built for Reproducibility

High-quality engineering includes Peltier-based thermal block system designed for superior thermal cycling and unmatched optical design to ensure every run is accurate and reproducible.

Unparalleled uniformity. Amplification curves for 96 replicates shown on a linear plot

(A) and a logarithmic plot (Figure B). In each well, 105 copies of GAPDH template were amplified in the presence of GAPDH primers and BioRad™ Sso Advanced SYBR Green Mix®. Average Cq = 19.1, Coefficient of variance (Cv)= 0.002.

Six-target Multiplex

Comfortably use up to 6 fluorescent channels, which allow for flexible dye usage and broad application. Because Rox is not required, it can also be used as a probe.

For In-vitro Diagnostics

Azure Cielo Dx

The Azure Cielo Dx qPCR system for IVD use brings you the accuracy and sensitivity you need for your research, with intuitive touch screen software. With 6-target detection, the flexibility you need is at your fingertips.

USA: For IVD use. The Azure Cielo 3 Dx and Azure Cielo 6 Dx Real-time PCR systems are registered with the U.S. FDA as Class II 510(k) exempt devices.

Europe:  For IVD use. The Azure Cielo 3 Dx and Azure Cielo 6 Dx systems are CE marked under the In Vitro Diagnostic Medical Devices Directive (98/79/EC). The CE IVD–authorized Azure Cielo Dx Systems are for distribution and use the following countries: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czechia, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Liechtenstein, Lithuania, Luxembourg, Malta, the Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, and the United Kingdom.


What Scientists are Saying

"Easy, accurate RT PCR machine with great software."


As a lab that has been carrying out high volume PCR testing for the last 12 months, the Azure Cielo Real-Time PCR machine has been a fantastic addition to our suite of equipment to facilitate this process. The comparison data we have achieved… more

"Super real time PCR."


It is a very user friendly instrument. A very rapid thermocycler, with very good reproducibility. A good instrument to scale and increase produtivity. We have 3 in our lab and I really recomend this real time PCR system for diferent aplications… more



Document TypeDescription
BrochureCielo BrochureDOWNLOAD
Application NoteUsing the Azure Cielo™ Real-Time PCR System for SARS-CoV-2 viral RNA detectionDOWNLOAD
Application Note6-Channel Multiplex qPCR Using Azure Cielo Real-Time PCR SystemDOWNLOAD
Application NoteSensitive and Accurate Single-Copy Amplicon Detection in Azure Cielo Real-Time PCR SystemDOWNLOAD
Application NoteAzure Cielo™ Real-Time PCR Optical Design Eliminates the Need for Passive Reference DyeDOWNLOAD
Application NoteWhat Sets the Cielo ApartDOWNLOAD
Application NoteDetection of low copy telomeric genes with CieloDOWNLOAD
Application NoteGoTaq Enviro Wastewater SARS-CoV-2DOWNLOAD
Application NoteqPCR Troubleshooting GuideDOWNLOAD
Technical NoteSNP Genotyping/Allelic DiscriminationDOWNLOAD
Technical NoteAdding Dyes and Color CompensationDOWNLOAD


Back to Basics: Experimental Design of Real-time PCR
How to Cielo: Setting up a Protocol


Azure Cielo is for research-use only, and not for use in diagnostic procedures. Contact us today for a quote and to learn more about how Azure is changing how researchers qPCR.

Part NumberProduct Name
AIQ315Azure Cielo 6 Dx qPCR (for In-vitro Diagnostics)REQUEST A QUOTE
AIQ314Azure Cielo 3 Dx qPCR (for In-vitro Diagnostics)REQUEST A QUOTE
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