Azure Cielo™ Real-time PCR system

qPCR detection system for fast run times, high-quality data, smart protocols, and robust durability

Azure Cielo Benefits


Email Notifications


Finally, qPCR data can be sent directly to your inbox after a run! Easily operates without use of cables. Cielo easily allows data transfer via Ethernet, Wi-Fi, or USB.




Engineered for a wide variety of qPCR applications with high sensitivity and wide dynamic range.​


Intelligent Workflow


User interface allows simple touchscreen assay setup. Saved protocols make assay set up easy and reproducible.


Reliable and Robust


Optics and thermal block are designed to deliver the same uniformity and reproducibility to last for up to 1,000 experiments and beyond.

Key Features

Email Notifications

Starting a run at the end of the day but need your data soon? Automatic email notifications from Cielo Manager have you covered.

Because the Cielo connects via Wi-Fi, its software not only notifies you when your run is completed, but sends the data straight to your inbox.

Quickly Set up and Confirm Plate Layout

Each well is easily labeled with three identifiers:

  1. Well Type,
  2. Sample ID, and
  3. Dye/Target.

These identifiers are used for data analysis and easy visualization of the amplification and standard curves.

View Each Individual Amplification Trace by Target or Sample

Easily view all of your data at once or select specific wells/targets to view a subset of your data.

Visualize amplification curves in linear or log view and change the color of the amplification curves to easily distinguish the data by Sample ID or target.

5-point, 10-fold dilution series of a six-target qPCR assay. Targets: RRP36 (Efficiency = 96.8%, R2 = 0.99), ACTB (Efficiency = 97.1%, R2 = 0.99), SDHA (Efficiency = 98.0%, R2 = 0.99), GAPDH (Efficiency = 100.8%, R2 = 0.99), TBP (Efficiency = 95.5%, R2 = 0.99), and B2M (Efficiency = 97.5%, R2 = 0.99).

2D Sample Heat Map

Wells are colored in a heat map style based on Cq value, with the lowest Cq values in green and highest Cq values in red. Quickly review Cq values, and assess data uniformity and separation between dilutions.

qPCR heatmap using Azure Cielo Manager
7-point, 10-fold dilution series in duplicate amplifying a 150 bp amplicon (Efficiency = 94.3%, R2 = 0.99), with two unknowns, each with four replicates.

Optical Design Eliminates the Need for ROX

Well-based excitation and color-specific LED light sources ensure the excitation efficiency in every well for every channel is the same. Coupled with the Cielo’s scanning technology which collects multiple data points from each well, the well-based optical design eliminates the need for normalization dyes such as ROX.

qPCR amplification curves using Azure Cielo Manager
Amplification curves for 96 replicates of the same 150 bp amplicon (Mean Cq = 19.5, Cq Standard deviation = 0.03).

Total Well Detection Technology Captures 100,000 Data Points per Well

While competitive systems are only able to acquire 1 pixel/well,  Cielo is able to capture approximately 100,000 pixels/wells, enabling accurate, reproducible and sensitive representation of fluorescent intensities from each qPCR well.

Cielo is able to capture approximately 100,000 pixels per qPCR well

Built for Reproducibility

High-quality engineering includes Peltier-based thermal block system designed for superior thermal cycling and unmatched optical design to ensure every run is accurate and reproducible.

Amplification curves for 96 replicates shown on a linear plot
Unparalleled uniformity. Amplification curves for 96 replicates of the same 150 bp amplicon: A. Linear view, B. Log view. (Mean Cq = 19.1, Cq Standard deviation = 0.001).

Six-target Multiplex

Multiplex up to six targets on the Cielo, a true six-channel system. Flexibility is built into the system for the use of numerous commercially available dyes and applications.

Because ROX is not required, it can also be used as a probe.

Azure Cielo Manager software showing six-target multiplexing
8-point, 10-fold standard curves for six targets. Targets: RRP36 (Efficiency = 103.6%, R2 = 0.96), ACTB (Efficiency = 100.5%, R2 = 0.99), RNAse P (Efficiency = 97.0%, R2 = 0.99), GAPDH (Efficiency = 100.2%, R2 = 1.00), TBP (Efficiency = 96.8%, R2 = 0.99), and EF1a (Efficiency = 97.2%, R2 = 0.99).

Choose Between Models

Compare Models (Dyes/Probes)

Other dyes/probes are possible depending on the system configuration.

For In-vitro Diagnostics

Azure Cielo Dx

The Azure Cielo Dx qPCR system for IVD use brings you the accuracy and sensitivity you need for your research, with intuitive touch screen software. With 6-target detection, the flexibility you need is at your fingertips.

USA: For IVD use. The Azure Cielo 3 Dx and Azure Cielo 6 Dx Real-time PCR systems are registered with the U.S. FDA as Class II 510(k) exempt devices.

Europe:  For IVD use. The Azure Cielo 3 Dx and Azure Cielo 6 Dx systems are CE marked under the In Vitro Diagnostic Medical Devices Directive (98/79/EC). The CE IVD–authorized Azure Cielo Dx Systems are for distribution and use the following countries: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czechia, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Liechtenstein, Lithuania, Luxembourg, Malta, the Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, and the United Kingdom.


What Scientists are Saying

"Easy, accurate RT PCR machine with great software."


As a lab that has been carrying out high volume PCR testing for the last 12 months, the Azure Cielo Real-Time PCR machine has been a fantastic addition to our suite of equipment to facilitate this process. The comparison data we have achieved… more

"Super real time PCR."


It is a very user friendly instrument. A very rapid thermocycler, with very good reproducibility. A good instrument to scale and increase produtivity. We have 3 in our lab and I really recomend this real time PCR system for diferent aplications… more

Experts Trust the Cielo qPCR System



Document TypeDescription
BrochureCielo BrochureDOWNLOAD
Application NoteUsing the Azure Cielo™ Real-Time PCR System for SARS-CoV-2 viral RNA detectionDOWNLOAD
Application Note6-Channel Multiplex qPCR Using Azure Cielo Real-Time PCR SystemDOWNLOAD
Application NoteSensitive and Accurate Single-Copy Amplicon Detection in Azure Cielo Real-Time PCR SystemDOWNLOAD
Application NoteAzure Cielo™ Real-Time PCR Optical Design Eliminates the Need for Passive Reference DyeDOWNLOAD
Application NoteWhat Sets the Cielo ApartDOWNLOAD
Application NoteDetection of low copy telomeric genes with CieloDOWNLOAD
Application NoteGoTaq Enviro Wastewater SARS-CoV-2DOWNLOAD
Application NoteAzure Cielo 6 Compatibility with GenDx KMRtype and KMRtrack WorkflowsDOWNLOAD
Technical NoteSNP Genotyping/Allelic DiscriminationDOWNLOAD
Technical NoteAdding Dyes and Color CompensationDOWNLOAD
Application NotePerformance of a six-target multiplexing assay on the Cielo™ 6 using the Quantabio PerfeCTa® Multiplex qPCR ToughMixDOWNLOAD
Application Note6-Channel Multiplex qPCR Using Azure Cielo Real-Time PCR SystemDOWNLOAD
User ManualAzure Cielo User ManualDOWNLOAD

Faster qPCR is within reach. Contact us today to get pricing on the Azure Cielo.

6747 Sierra Court, Suite A-B
Dublin, CA 94568
United States

Tel: (925) 307 7127
Fax: (925) 905-1816

Copyright © 2023 Azure Biosystems Inc.
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