Azure BiosystemsHigh-quality and affordable reagents, imagers, and qPCR systems
Azure Biosystems
qPCR system for in-vitro diagnostic use, bringing accuracy and sensitivity for research
The Azure Cielo Dx Real-time PCR system for in-vitro diagnostic use operates with 6-target detection. The flexibility you need is at your fingertips.
Our 3 or 6 standard channels let you analyze a variety of commercially available dyes, already in your workflow. Onboard software operated by a 10.2" touchscreen minimizes footprint and saves you bench space.
USA: For in vitro diagnostic (IVD) use. The Azure Cielo 3 Dx and Azure Cielo 6 Dx Real-time PCR systems are registered with the U.S. FDA as Class II 510(k) exempt devices.
EU: For IVD use. The Azure Cielo 3 Dx and Azure Cielo 6 Dx systems are CE marked under the In Vitro Diagnostic Medical Devices Directive (98/79/EC). The CE IVD–authorized Azure Cielo Dx Systems are for distribution and use in the following countries: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czechia, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Liechtenstein, Lithuania, Luxembourg, Malta, the Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden
 | | |
|---|---|---|
| Azure Cielo 3 | Azure Cielo 6 | |
| SAMPLE CAPACITY (WELLS) | 96 | 96 |
| COMPATIBLE PLATES/TUBES | 96-WELL PLATES & TUBES AND 8 OR 12-WELL STRIPS & SINGLE TUBES | 96-WELL PLATES & TUBES AND 8 OR 12-WELL STRIPS & SINGLE TUBES |
| REACTION VOLUME | 5–150uL | 5–150uL |
| EXCITATION SOURCE | LED, EXCITATION RANGE: 450–580NM AND 610–700NM | LED, EXCITATION RANGE: 450–730NM |
| DETECTION CHANNELS | 3 | 6 |
| MULTIPLEXING | UP TO 3 TARGETS | UP TO 6 TARGETS |
| REACTION TEMPERATURE | 4°C TO 99.9°C | 4°C TO 99.9°C |
| MAX. BLOCK RAMP RATE | 6°C/SEC | 6°C/SEC |
| AVG. SAMPLE RAMP RATE | 4°C/SEC | 4°C/SEC |
| TEMPERATURE UNIFORMITY | ±0.2°C | ±0.2°C |
| TEMPERATURE ACCURACY | ±0.1°C | ±0.1°C |
| CUSTOM DYE/CHEMISTRY |  | |
| ONBOARD MEMORY | 32GB EQUIVALENT TO 20,000 EXPERIMENTS | 32GB EQUIVALENT TO 20,000 EXPERIMENTS |
| CONNECTIVITY | USB, WI-FI, ETHERNET | USB, WI-FI, ETHERNET |
| FOOTPRINT (W/D/H) | 12" X 20" X 17" (30.5 X 50.8 X 43.2 CM) | 12" X 20" X 17" (30.5 X 50.8 X 43.2 CM) |
Each well is color coded by sample type, biological replicate, and technical replicate. Well type and standard curve values are also easily visible.
NIR lasers keep signal high and background low. Our high-performance multiplex NIR lasers and filters deliver robust excitation energy which maximizes emission strength for maximum sensitivity.
This feature makes it easy to review your samples and quickly understand your data.
The Cielo’s efficient well-based scanning technology scans multiple data points within each well, eliminating the need for normalization dyes such as ROX.
Amplification curves generated using human genomic DNA template along with human GAPDH primers and a no ROX SYBR Green qPCR mix.
Competitive systems only acquire 1 pixel/well compared to the Cielo’s ability to capture approximately 100,000 pixels/wells, enabling accurate, reproducible and sensitive representation of fluorescent intensities from each qPCR well.
High-quality engineering includes Peltier-based thermal block system designed for superior thermal cycling and unmatched optical design to ensure every run is accurate and reproducible.
Unparalleled uniformity. Amplification curves for 96 replicates shown on a linear plot
(A) and a logarithmic plot (Figure B). In each well, 105 copies of GAPDH template were amplified in the presence of GAPDH primers and BioRad™ Sso Advanced SYBR Green Mix®. Average Cq = 19.1, Coefficient of variance (Cv)= 0.002.
| Document Type | Description | |
|---|---|---|
| Brochure | Cielo Dx Brochure | DOWNLOAD |
| Press Release | Azure Biosystems Launches Azure Cielo Dx for In-vitro Diagnostic Use | DOWNLOAD |
| Application Note | Optical Design Eliminates the Need for ROX Passive Reference Dye | DOWNLOAD |
| Application Note | Sensitive and Accurate Single-Copy Amplicon Detection | DOWNLOAD |
| Application Note | Data for 6-Channel Multiplex qPCR | DOWNLOAD |
| Application Note | Using the Azure Cielo™ Real-Time PCR System for SARS-CoV-2 viral RNA detection | DOWNLOAD |
| Application Note | GoTaq Enviro Wastewater | DOWNLOAD |
| Part Number | Product Name | |
|---|---|---|
| AIQ315 | Azure Cielo 6 Dx qPCR | REQUEST A QUOTE |
| AIQ314 | Azure Cielo 3 Dx qPCR | REQUEST A QUOTE |
