Azure BiosystemsHigh-quality and affordable reagents, imagers, and qPCR systems
Azure BiosystemsqPCR system for in-vitro diagnostic use, bringing accuracy and sensitivity for research
The Azure Cielo Dx qPCR system for in-vitro diagnostic (IVD) use operates with 6-target detection. The flexibility you need is at your fingertips.
The option of 3 or 6 standard channels lets you analyze a variety of commercially available dyes, already in your workflow.
USA: For IVD use. The Azure Cielo 3 Dx and Azure Cielo 6 Dx Real-time PCR systems are registered with the U.S. FDA as Class II 510(k) exempt devices.
EU: For IVD use. The Azure Cielo 3 Dx and Azure Cielo 6 Dx systems are CE marked under the In Vitro Diagnostic Medical Devices Directive (98/79/EC). The CE IVD–authorized Azure Cielo Dx Systems are for distribution and use in the following countries: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czechia, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Liechtenstein, Lithuania, Luxembourg, Malta, the Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden
SAMPLE CAPACITY (WELLS)
96
COMPATIBLE PLATES/TUBES
96-WELL PLATES & TUBES AND 8
OR 12-WELL STRIPS & SINGLE TUBES
REACTION VOLUME
1-150 uL (10-65 uL recommended)
DYNAMIC RANGE
10 LOGS
EXCITATION SOURCE
LED, EXCITATION RANGE: 450–580NM AND 610–700NM
DETECTION CHANNELS
3
MULTIPLEXING
UP TO 3 TARGETS
REACTION TEMPERATURE
4°C to 99.9°C
MAX. BLOCK RAMP RATE
6°C/sec
AVG. SAMPLE RAMP RATE
4°C/SEC
TEMPERATURE UNIFORMITY
±0.2°C
TEMPERATURE ACCURACY
±0.1°C
CUSTOM DYE/CHEMISTRY
✓
ONBOARD MEMORY
32GB Equivalent to 20,000 EXPERIMENTS
CONNECTIVITY
USB, WI-FI, ETHERNET
FOOTPRINT (W/D/H)
12″ X 20″ X 17″ (30.5 X 50.8 X 43.2 CM)
SAMPLE CAPACITY (WELLS)
96
COMPATIBLE PLATES/TUBES
96-WELL PLATES & TUBES AND 8
OR 12-WELL STRIPS & SINGLE TUBES
REACTION VOLUME
1-150 uL (10-65 uL recommended)
DYNAMIC RANGE
10 LOGS
EXCITATION SOURCE
LED, EXCITATION RANGE: 450–730NM
DETECTION CHANNELS
6
MULTIPLEXING
UP TO 6 TARGETS
REACTION TEMPERATURE
4°C to 99.9°C
MAX. BLOCK RAMP RATE
6°C/sec
AVG. SAMPLE RAMP RATE
4°C/SEC
TEMPERATURE UNIFORMITY
±0.2°C
TEMPERATURE ACCURACY
±0.1°C
CUSTOM DYE/CHEMISTRY
✓
ONBOARD MEMORY
32GB EQUIVALENT TO 20,000 EXPERIMENTS
CONNECTIVITY
USB, WI-FI, ETHERNET
FOOTPRINT (W/D/H)
12″ X 20″ X 17″ (30.5 X 50.8 X 43.2 CM)
Each well is color coded by sample type, biological replicate, and technical replicate. Well type and standard curve values are also easily visible.
NIR lasers keep signal high and background low. Our high-performance multiplex NIR lasers and filters deliver robust excitation energy which maximizes emission strength for maximum sensitivity.
This feature makes it easy to review your samples and quickly understand your data.
The Cielo’s efficient well-based scanning technology scans multiple data points within each well, eliminating the need for normalization dyes such as ROX.
Amplification curves generated using human genomic DNA template along with human GAPDH primers and a no ROX SYBR Green qPCR mix.
Competitive systems only acquire 1 pixel/well compared to the Cielo’s ability to capture approximately 100,000 pixels/wells, enabling accurate, reproducible and sensitive representation of fluorescent intensities from each qPCR well.
High-quality engineering includes Peltier-based thermal block system designed for superior thermal cycling and unmatched optical design to ensure every run is accurate and reproducible.
Unparalleled uniformity. Amplification curves for 96 replicates shown on a linear plot
(A) and a logarithmic plot (Figure B). In each well, 105 copies of GAPDH template were amplified in the presence of GAPDH primers and BioRad™ Sso Advanced SYBR Green Mix®. Average Cq = 19.1, Coefficient of variance (Cv)= 0.002.
Good machine, easy to use, highly accurate and easy to take the results but the difficulty in test timing it takes an hour and 37 minutes to complete the run.
| Document Type | Description | |
|---|---|---|
| Brochure | Azure Cielo Dx Brochure | DOWNLOAD |
| Press Release | Azure Biosystems Launches Azure Cielo Dx for In-vitro Diagnostic Use | DOWNLOAD |
| Application Note | Optical Design Eliminates the Need for ROX Passive Reference Dye | DOWNLOAD |
| Application Note | Sensitive and Accurate Single-Copy Amplicon Detection | DOWNLOAD |
| Application Note | Data for 6-Channel Multiplex qPCR | DOWNLOAD |
| Application Note | Using the Azure Cielo™ Real-Time PCR System for SARS-CoV-2 viral RNA detection | DOWNLOAD |
| Application Note | GoTaq Enviro Wastewater | DOWNLOAD |
| Part Number | Product Name | |
|---|---|---|
| AIQ315 | Azure Cielo 6 Dx qPCR | REQUEST A QUOTE |
| AIQ314 | Azure Cielo 3 Dx qPCR | REQUEST A QUOTE |
