Azure BiosystemsBlog Posts in this category:
Your common Western blotting questions, answered. Western blotting is a widely used analytical technique that can identify one or more specific proteins in a complex
The regulation of gene expression is a complicated affair. A vast array of control mechanisms exist that can adjust the levels of gene expression products
How can we better control pathogenic bacteria? Insights may come from studying bacteriophages, viruses that infect bacteria. There are a wide variety of bacteriophages, each
Ponceau S is a fast, reversible protein stain often used to confirm that protein samples have successfully transferred from the gel to the membrane before
Have you ever spent ages correctly quantifying your protein samples, casting the perfect gel, efficiently transferred, probed and eventually come to the visualization stage only
Today’s post is inspired by a conversation I had with a colleague when teaching them SDS-PAGE and Western blotting. Being well versed in qRT-PCR (R)
Validating your antibodies, i.e. confirming an antibody recognizes your protein of interest with low cross-reactivity to other targets, is critical for ensuring consistent, reproducible results.
Best practices for quantitation of unphosphorylated vs phosphorylated forms In part 1, we reviewed tips for using multiplexing to visualize phosphoproteins. In part 2, we’re
Western blotting is an important tool for researchers studying signal transduction and other processes where understanding the phosphorylation state of a protein is important for
In a recent publication, Ji Xi et al examined the role of the linker region of the Hepatitis B virus (HBV) core protein, a protein
