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Best practices for quantitation of unphosphorylated vs phosphorylated forms In part 1, we reviewed tips for using multiplexing to visualize phosphoproteins. In part 2, we’re
Western blotting is an important tool for researchers studying signal transduction and other processes where understanding the phosphorylation state of a protein is important for
Western blots, step aside… Although Western blotting is an established technique for detecting a specific protein within a complex sample, it has several inherent shortcomings.
Azure offers products to support every step of your Western blotting workflow, from blocking to detection. Several recent publications highlight the use of Azure reagents
Far from being an “is-it-there-or-not” technique, modern digital detection instruments can make Western blotting reproducible and quantitative. By working within the linear dynamic range of
When quantifying Western blots many people assume it’s ok to simply image your protein of interest, strip, re-probe for their loading control of choice and