Blog Posts in this category:
Best practices for quantitation of unphosphorylated vs phosphorylated forms Figure 1. AzureSpot Multiplex Image In part 1 we reviewed tips for using multiplexing to visualize
Figure 1. Digital images of 4-color Western Blot. Using distinct fluorescent and near-infrared targeting antibodies can detect each wavelength and merge them into a four-color
Western blots, step aside… Although Western blotting is an established technique for detecting a specific protein within a complex sample, it has several inherent shortcomings.
Azure offers products to support every step of your Western blotting workflow, from blocking to detection. Several recent publications highlight the use of Azure reagents
Far from being an “is-it-there-or-not” technique, modern digital detection instruments can make Western blotting reproducible and quantitative. By working within the linear dynamic range of
When quantifying Western blots many people assume it’s ok to simply image your protein of interest, strip, re-probe for their loading control of choice and