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Loading controls and antibody validation for Western blot quantification
Today’s post is inspired by a conversation I had with a colleague when teaching them SDS-PAGE and Western blotting. Being well versed in qRT-PCR (R)
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Today’s post is inspired by a conversation I had with a colleague when teaching them SDS-PAGE and Western blotting. Being well versed in qRT-PCR (R)
Best practices for quantitation of unphosphorylated vs phosphorylated forms Figure 1. AzureSpot Multiplex Image In part 1 we reviewed tips for using multiplexing to visualize
Figure 1. Digital images of 4-color Western Blot. Using distinct fluorescent and near-infrared targeting antibodies can detect each wavelength and merge them into a four-color
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