One system for:
*available with optional Chemiluminescent Module
It’s time to consider a new idea, an innovative system with proven results! Instead of taking the easy path and sticking with the same vendor, make the switch to a company that fully supports your research. Azure Biosystems recently launched the new Sapphire FL, which delivers more than the Cytiva Typhoon. The Sapphire FL offers:
Visible Fluorescence
NIR Fluorescence
Phosphor Imaging
Chemiluminescence*
Fluorescence Detectors: APD + PMT
Automatic Z-plane Imaging Tool
Fluorescence imaging area: 25 x 25 cm
Laser diode lifespan: 40,000 hours
Resolution: 5-1000 µm
Non-proprietary Image Formats
Dynamic Range: > 6 logs
We purchased the Sapphire Imager to replace our obsolete Typhoon Scanner and could not be happier with the performance and ease of use. Equipped with multiple lasers, it is possible to probe for many proteins at high resolution and with great sensitivity. We primarily use the system for western blotting and fluorescent slide scanning and have had clear, publishable results. The software is easy to use and has many practical features. Customer service is amazing – they are always available, informative, and as scientists, they assist with troubleshooting experimental design and optimizing results.
The new Sapphire FL offers customizable and user-changeable optical modules, 5–1000 μm resolution scans, a Z-plane range from -1.0 to +6 mm, 5 anesthesia output ports for imaging living animals, chemiluminescence detection through an optional Chemiluminescence Module and much more.
In addition to fluorescence, the Sapphire FL also excels at imaging phosphor screens. The flexibility of the Sapphire FL guarantees you the freedom to confidently choose the best dyes and chemistry for any experiment.
The Sapphire FL Z-plane ranges from -1 mm below to +6 mm above the glass imaging surface, providing the flexibility to image any sample with optimal focus. Additionally, easily create Z-stacks and GIFs to quickly visualize and share data across a range of focal planes.
Southern blots | Northern blots | Western blots | Multiplex Westerns | Quantitative Westerns | Total Protein Normalization | In-cell Westerns | Agar plates/Clonogenic assays | Multi-well plate imaging | Protein arrays | Microarrays | ELISAs | Immunohistochemistry | Lateral flow immunoassay development | Thin layer chromatography imaging | Electrophoretic mobility shift assays (EMSA) | 2D DIGE | Densitometry | Gel documentation | In-gel imaging | DNA gel imaging | RNA gel imaging | Protein gel imaging | Coomassie imaging | Silver-stain imaging | Fluorescent gel stain imaging | Gel autoradiography | Membrane autoradiography | Tissue section autoradiography | Tissue section imaging | Plant bioluminescence imaging | Phosphoprotein studies | Glycoprotein assays | Reporter gene assays | GFP expression in model organisms | Small animal imaging
Sapphire™ | Amersham® Typhoon® | ||
---|---|---|---|
NIR Fluorescence | ![]() | ![]() | |
Visible Fluorescence | ![]() | ![]() | |
Phosphor Imaging | ![]() | ![]() | |
Software-controlled, fine z-axis | ![]() | ||
Ability to switch between sequential and simultaneous scanning | ![]() | ||
True chemiluminescence imaging using a CCD with the same sensitivity as film | ![]() | ||
Resolution | 10µm | 10µm | |
Dynamic Range | > 6 logs | > 5 logs | |
Price | $ | $$ |
Fluorescent Detection Method
APD only
NIR Fluorescence
✅
Visible Fluorescence
✅
Phosphor Imaging
✅
Chemiluminescence
❌
Automatic Z-plane Imaging Tool
❌
Maximum resolution
10µm
Dynamic Range
> 5 logs
Table 1. The Azure Sapphire FL Biomolecular Imager offers the greatest variety of excitation wavelengths of the instruments in its class.
Amersham Typhoon™ is a registered trademark of Cytiva. No endorsement is implied by the use of these marks. All rights reserved. The Azure Biosystems logo, Azure Biosystems™ and Azure Sapphire™ are trademarks of Azure Biosystems, Inc.
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