The Sky is the Limit

Start Your 7-day Free Trial of the Azure Cielo™

Multiplex is the future. Join countless satisfied users around the globe by experiencing what the Cielo has to offer. Its flexible and user-friendly software caters to your specific needs, and the onboard software that’s operated by a 10.2″ touchscreen minimizes its footprint and saves you bench space.

The Cielo is unparalleled in flexibility, speed, sensitivity, reproducibility, and simplicity. Our novel fiber optic detection system allows for enhanced sensitivity and speed, and six channels allow you to analyze a variety of commercially available dyes that are already in your workflow.

Discover how the Cielo can transform your own research. Fill in the request form for the opportunity to try the Cielo in your own lab for one week.

Applications

  • Quantitative and qualitative gene expression analysis
  • miRNA analysis
  • Genetic mapping
  • Genetic fingerprinting
  • NGS library quantification
  • 3- or 6-channel multiplex ability
  • Pathogen quantification

Key Features

  • 1-year standard warranty
  • 3 or 6 optical channels for multiplexing
  • Tested durability up to 1000 repeat experiments
  • Marlow Peltier heating block with 12 gradient temperature zones
  • 6.0 °C/sec ramp rate
  • 96-well format for semi-skirted or un-skirted plates
  • Fiber-optic detection for 16-wells simultaneously
  • Sensitivity up to 1 copy of amplified DNA
  • 10 logs of dynamic range
  • Open source chemistry, including approximately 20-minute run times
  • Flexible data export options through Wi-Fi, ethernet, USB, or LAN run-time. Automatic notifications after each completion

Innovative Optical Design

The Cielo uses an innovative optic system that allows for dedicated well scanning with higher excitation and detection efficiency. You are able to detect more than 100,000 data points within a given well. Using the Cielo increases experimental reliability and reproducibility.

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Brilliant by Design

Single-well excitation and detection is specific and precise for detecting qPCR signals. No excitation light falls outside of the well, reducing excitation of non-well regions. This reduces signal masking background and eliminates the need to use passive reference dyes, such as ROX.

Multiplex qPCR reactions were scanned for all 6 channels in with a Cielo. Single channel amplification curves were as expected, with no leakage or cross-talk.

More light is required to cover more surface area of the plate thereby dropping the excitation efficiency required for the actual qPCR reaction. To compensate for background, passive reference dyes such as ROX are required.

"Easy, accurate RT PCR machine with great software."

5/5

As a lab that has been carrying out high volume PCR testing for the last 12 months, the Azure Cielo Real-Time PCR machine has been a fantastic addition to our suite of equipment to facilitate this process. The comparison data we have achieved… more

Documents

Document TypeDescription
BrochureCielo BrochureDOWNLOAD
Application NoteOptical Design Eliminates the Need for ROX Passive Reference DyeDOWNLOAD
Application NoteSensitive and Accurate Single-Copy Amplicon DetectionDOWNLOAD
Application NoteData for 6-Channel Multiplex qPCRDOWNLOAD
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