Ultimate Western Blot Imaging System

Your Western Blot method shouldn’t be limited by your Western blot imager! With the Azure 600, it’s not.

Two scientists looking at screen on Azure 600 Western blot imager

Azure 600® | The All-around Application Ace

NIR
RGB
Chemi
Blue Light

White Light

Ultraviolet

Two-channel NIR Fluorescence Detection

Western Blot Imager with Multiplex Capabilities, and the Best Option for Quantitation

The Azure 600 is the only system that offers two channel laser based IR detection, chemiluminescent detection with the speed and sensitivity of film, the ability to image visible fluorescent dyes, and the ability to image standard EtBr and protein gels. Infrared laser excitation for quantitative Western blot imaging in the NIR. This Western blot imager improves your data quality imaging with infrared dyes and offers signal stability. Low background fluorescence imaging with NIR dyes allows you to study multiple proteins in a blot, even if those proteins overlap in molecular weight.

The Azure 600 offers laser technology with two IR detection channels enabling a user to image more than one protein in an assay. The Azure 600 provides accurate and fast chemiluminescent detection, as well as the sensitivity, dynamic range, and linearity needed for quantitative blot analysis.

Two-fold Serial Dilution
Two-fold serial dilutions of AzureSpectra-800 labeled antibody were spotted on a nitrocellulose dot-blot, and imaged in the 800nm channel for 20 seconds
Fluorescent Western Blot of STAT1 and phospho-STAT1
Fluorescent Western Blot of STAT1 and phospho-STAT1. The blot was probed with anti-phospho-STAT1 and anti-STAT1 followed by fluorescent secondary antibodies, and then imaged on Azure Imaging system. Top right is the green channel, using IR-800; top left is the image of the red channel, using IR-700. Bottom image is both channels merged.

Three-channel RGB Fluorescent Detection

Fluorescent protein in native gel.
Fluorescent protein in native gel imaged with an Azure imager
Zebrafish Expressing GFP
Zebrafish Expressing GFP - imaged with an Azure Imager
Mouse with RFP-expressing subcutaneous tumor.
Mouse with RFP-expressing Subcutaneous Tumor - imaged with an Azure Imager
GFP- and mCherry-expressing E. coli.
GFP- and mCherry-expressing E. coli. imaged with an Azure Imager
Intuitive capture software controlled by a wide touchscreen

Every Azure Imaging System provides high-resolution imaging that is ideal for publication purposes, as well as higher pixel well cap for higher dynamic range.

Use the preset protocols of the comprehensive list of typical detections, or generate your own custom protocols combining any excitation source, emission filter, image overlay, lens aperture, exposure time, focus, distance to the camera, and resolution.

High Resolution Imaging

A 9.1MP camera provides high resolution imaging perfect for publications. Change the sample to optics distance using adjustable height shelf for enhanced detection. Zoom into the area of interest with ROI imaging to reduce background.

Sensitivity Signal Surpasses Film

Revolutionize Your Chemiluminescent Workflows by Leaving the Darkroom

Quantitative Chemiluminescent Imaging

Get the best picture every time, and stop wasting money on film. Use Cy®5/Cy®3/Cy®2 Dyes, Blue-Excited DNA Dyes, and more. A Total Solution for Western Blot Imaging and Gel Documentation The Azure 600 is a multichannel, multimodal imager, with IR, visible light, and UV excitation channels. Detect Cy dyes, Alexa® dyes, Safe dyes, and more.

With Azure Imaging systems, the software notifies you when bands are saturated and are not suitable for quantitation.

Figure 1. Saturation detection prevents errors in quantitation. The same Western Blot was imaged on both x-ray film and the Azure Imaging System. The Azure system detects when CCD saturation occurs and calculates an auto-exposure time to avoid saturated bands.
Chemiluminescent Imaging with Color Markers

Every Azure Imaging System provides high-resolution imaging that is ideal for publication purposes, as well as higher pixel well cap for higher dynamic range.

You can overlay a color marker with the chemiluminescent signal for ease of documentation and molecular size determination.

 

Chemiluminescent Western blot with color marker imaged with Azure Biosystems
The Azure 600 gives you the flexibility you need for your research, while delivering solutions for quantitative Western Blot imaging. Azure Biosystems provides a unified Western Blot workflow, from the high-performance imaging system and analysis software to the reagents and consumables.
Azure 600 Western blot Imaging system

The Azure 600 Western Blot Imager Offers:

Flexibility—Sensitivity and performance for fluorescence, chemiluminescence, trans-UV & visible, and true-color imaging with 4.8 OD dynamic range. High resolution CCD cameras with fast lens options, adjustable lens and illumination settings, trays with adjustable distance to the camera, and auto-detection and pre-calibrated focus.

Quantitative accuracy—Designed for generating publication quality images and easy quantitation. Our reagents, imaging system, and software work seamlessly together to help you follow best practices for Western Blot publication.

Intelligent workflow—Our user interface allows total customization over imaging protocols, while ensuring repeatability from sample to sample. Our systems feature Auto-Focus, Auto-Illumination and Filter Control, and Auto-Image Capture. An integrated touchscreen allows ease of use and it can easily be controlled by an external PC, if required.

Data integrity—Azure Biosystems meets the standards for publication in all major journals, and additionally offers software to enable 21 CFR Part 11 Compliance.

Publications

Literature

Document TypeDescription
BrochureAzure Imager BrochureDOWNLOAD
BrochureAzure Imaging Systems Model ConfigurationsDOWNLOAD
User ManualAzure Imaging Systems User ManualDOWNLOAD

Compare Western Blot Imaging Systems

Choose the configuration that supports today’s studies, while maintaining the option to upgrade later on

Upgrades available for chemiluminescence, NIR fluorescence and RGB fluorescence.

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