The top fluorescent imager that’s the best solution for sensitive near-infrared and ECL Western blots, and gel documentation.
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The Azure 500’s laser technology offers two IR detection channels for the ability to image more than one protein in an assay. The Azure 500 provides accurate and fast chemiluminescent detection, as well as the sensitivity, dynamic range, and linearity needed for quantitative blot analysis. Get the best picture every time, and stop wasting money on film! UV, color imaging, blue-excited DNA dyes, Coomassie gel imaging, and more a total solution for Western blot imaging and gel documentation.
The Azure 500 is a multichannel, multimodal fluorescent imager, with IR, visible light, and UV excitation channels. Image and quantify two different targets in the same position more efficiently using Near-Infrared (NIR), and normalize to fluorescent total protein stain or a housekeeping protein in the green channel without needing to strip and re-probe your Western blots.
Two NIR fluorescence channels and laser diodes at 685nm and 784nm offer better sensitivity than LEDs or white light sources for Western Blot.
The Azure 500 enables sensitive multiplex Western blotting with minimal background through two-channel NIR fluorescence detection. Capture images of NIR fluorescence WB in multiplex, ECL WB, biomolecules tagged with fluorescent labels in gels, fluorescent and colorimetric stains in gels, Petri dishes, TLC, etc.
The Azure 500 provides high-resolution imaging that is ideal for publication purposes, as well as higher pixel well cap for higher dynamic range.
Use the preset protocols of the comprehensive list of typical detections, or generate your own custom protocols combining any excitation source, emission filter, image overlay, lens aperture, exposure time, focus, distance to the camera, and resolution.
A 9.1MP camera provides high resolution imaging perfect for publications. Change the sample to optics distance using adjustable height shelf for enhanced detection. Zoom into the area of interest with ROI imaging to reduce background.
The software on the Azure 500 notifies you when bands are saturated and are not suitable for quantitation. Outperforms film performance while generates a quantitative, wide dynamic range image. Indicates eventual signal saturation for accurate quantitation.
The Azure 500 fluorescent imager provides high-resolution imaging that is ideal for publication purposes, as well as higher pixel well cap for higher dynamic range.
It gives you the option to overlay a color marker with the chemiluminescent signal for ease of documentation and molecular size determination.
The Azure 500 can be field upgraded to the Azure 600 for applications detecting fluorescent biomolecules in the visible range, Cy2/Cy3/Cy5, or similar fluorochromes.
Add a Q Module for Total Protein Normalization of NIR Western blots using TotalStain Q, and detection of one more fluorescent target.
• Flexibility—Sensitivity and performance for fluorescence, chemiluminescence, trans-UV & visible, and true-color imaging with 4.8 OD dynamic range. High resolution CCD cameras with fast lens options, adjustable lens and illumination settings, trays with adjustable distance to the camera, and auto-detection and pre-calibrated focus.
• Quantitative accuracy—Designed for generating publication quality images and easy quantitation. Our reagents, imaging system, and software work seamlessly together to help you follow best practices for Western Blot publication.
• Intelligent workflow—Our user interface allows total customization over imaging protocols, while ensuring repeatability from sample to sample. Our systems feature Auto-Focus, Auto-Illumination and Filter Control, and Auto-Image Capture. An integrated touchscreen allows ease of use and it can easily be controlled by an external PC, if required.
• Data integrity—The Azure 500 meets the standards for publication in all major journals, and additionally offers software to enable 21 CFR Part 11 Compliance.
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