Multiplex Capabilities, and the Best Option for Quantitation
The two-channel Near-Infrared (NIR) Fluorescence detection enables sensitive multiplex Western Blotting with minimal background. Capture images of NIR fluorescence WB in multiplex, ECL WB, biomolecules tagged with fluorescent labels in gels, fluorescent and colorimetric stains in gels, Petri dishes, TLC, etc.
Two NIR fluorescence channels and laser diodes at 685nm and 784nm offer better sensitivity than LEDs or white light sources for Western Blot.
Image and quantify two different targets in the same position more efficiently using NIR, and normalize to fluorescent total protein stain or a housekeeping protein in the green channel without needing to strip and re-probe your Western blots.
Fast and Sensitive Chemiluminescent Detection
With Azure Imaging systems, the software notifies you when bands are saturated and are not suitable for quantitation. Outperforms film performance while generates a quantitative, wide dynamic range image. Indicates eventual signal saturation for accurate quantitation.
Every Azure Imaging System provides high-resolution imaging that is ideal for publication purposes, as well as higher pixel well cap for higher dynamic range.
You can overlay a color marker with the chemiluminescent signal for ease of documentation and molecular size determination.
The Azure 500 can be field upgraded to the Azure 600 for applications detecting fluorescent biomolecules in the visible range, Cy2/Cy3/Cy5, or similar fluorochromes.
Add a Q Module for Total Protein Normalization of Near-Infrared WB using TotalStain Q, and detection of one more fluorescent target.
• Flexibility—Sensitivity and performance for fluorescence, chemiluminescence, trans-UV & visible, and true-color imaging with 4.8 OD dynamic range. High resolution CCD cameras with fast lens options, adjustable lens and illumination settings, trays with adjustable distance to the camera, and auto-detection and pre-calibrated focus.
• Quantitative accuracy—Designed for generating publication quality images and easy quantitation. Our reagents, imaging system, and software work seamlessly together to help you follow best practices for Western Blot publication.
• Intelligent workflow—Our user interface allows total customization over imaging protocols, while ensuring repeatability from sample to sample. Our systems feature Auto-Focus, Auto-Illumination and Filter Control, and Auto-Image Capture. An integrated touchscreen allows ease of use and it can easily be controlled by an external PC, if required.
• Data integrity—Azure Biosystems meets the standards for publication in all major journals, and additionally offers software to enable 21 CFR Part 11 Compliance.