Ultimate Infrared Imaging System

The Azure 500 is the best solution for sensitive near-infrared and ECL Western Blots, and gel documentation.

Two-channel NIR Fluorescence Detection

Multiplex Capabilities, and the Best Option for Quantitation

The two-channel Near-Infrared (NIR) Fluorescence detection enables sensitive multiplex Western Blotting with minimal background. Capture images of NIR fluorescence WB in multiplex, ECL WB, biomolecules tagged with fluorescent labels in gels, fluorescent and colorimetric stains in gels, Petri dishes, TLC, etc.

Two NIR fluorescence channels and laser diodes at 685nm and 784nm offer better sensitivity than LEDs or white light sources for Western Blot.

Image and quantify two different targets in the same position more efficiently using NIR, and normalize to fluorescent total protein stain or a housekeeping protein in the green channel without needing to strip and re-probe your Western blots.

Two-fold Serial Dilution
Two-fold serial dilutions of AzureSpectra-800 labeled antibody were spotted on a nitrocellulose dot-blot, and imaged in the 800nm channel for 20 seconds
Fluorescent Western Blot of STAT1 and phospho-STAT1
Fluorescent Western Blot of STAT1 and phospho-STAT1. The blot was probed with anti-phospho-STAT1 and anti-STAT1 followed by fluorescent secondary antibodies, and then imaged on Azure Imaging system. Top right is the green channel, using IR-800; top left is the image of the red channel, using IR-700. Bottom image is both channels merged.
Fluorescent protein in native gel.
Fluorescent protein in native gel imaged with an Azure imager
Zebrafish Expressing GFP - imaged with an Azure Imager
Mouse with RFP-expressing subcutaneous tumor.
Mouse with RFP-expressing Subcutaneous Tumor - imaged with an Azure Imager
GFP- and mCherry-expressing E. coli.
GFP- and mCherry-expressing E. coli. imaged with an Azure Imager

Sensitivity Signal Surpasses Film

Fast and Sensitive Chemiluminescent Detection

Quantitative Chemiluminescent Imaging

With Azure Imaging systems, the software notifies you when bands are saturated and are not suitable for quantitation. Outperforms film performance while generates a quantitative, wide dynamic range image. Indicates eventual signal saturation for accurate quantitation.

Figure 1. Saturation detection prevents errors in quantitation. The same Western Blot was imaged on both x-ray film and the Azure Imaging System. The Azure system detects when CCD saturation occurs and calculates an auto-exposure time to avoid saturated bands.
Chemiluminescent Imaging with Color Markers

Every Azure Imaging System provides high-resolution imaging that is ideal for publication purposes, as well as higher pixel well cap for higher dynamic range.

You can overlay a color marker with the chemiluminescent signal for ease of documentation and molecular size determination.

 

Upgradeable to Best Cater to Your Studies

Upgradeable to include three RGB fluorescence channels

The Azure 500 can be field upgraded to the Azure 600 for applications detecting fluorescent biomolecules in the visible range, Cy2/Cy3/Cy5, or similar fluorochromes.

*Optional Q Module (Green Channel)

Add a Q Module for Total Protein Normalization of Near-Infrared WB using TotalStain Q, and detection of one more fluorescent target.

500® | The Ultimate IR Imaging System
NIR

Chemi

Blue Light
white light
Ultraviolet
Upgradeable
The two-channel NIR Fluorescence detection enables sensitive multiplex Western Blotting with minimal background. Image and quantify two different targets in the same position more efficiently without needing to strip and re-probe your WBs.
Azure 500
Each Azure Imaging System offers:


Flexibility—Sensitivity and performance for fluorescence, chemiluminescence, trans-UV & visible, and true-color imaging with 4.8 OD dynamic range. High resolution CCD cameras with fast lens options, adjustable lens and illumination settings, trays with adjustable distance to the camera, and auto-detection and pre-calibrated focus.

Quantitative accuracy—Designed for generating publication quality images and easy quantitation. Our reagents, imaging system, and software work seamlessly together to help you follow best practices for Western Blot publication.

Intelligent workflow—Our user interface allows total customization over imaging protocols, while ensuring repeatability from sample to sample. Our systems feature Auto-Focus, Auto-Illumination and Filter Control, and Auto-Image Capture. An integrated touchscreen allows ease of use and it can easily be controlled by an external PC, if required.

Data integrity—Azure Biosystems meets the standards for publication in all major journals, and additionally offers software to enable 21 CFR Part 11 Compliance.

Literature

Document TypeDescription
BrochureAzure Imager BrochureDOWNLOAD
BrochureAzure Imaging Systems Model ConfigurationsDOWNLOAD
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