Visible Fluorescent Imager

Azure 400 is a flexible fluorescent imager, enabling three color fluorescent detection for dyes in the visible range.

Azure 400 Visible fluorescent imager

Azure 400® | The Visible Fluorescence Virtuoso

RGB
Chemi
Blue Light

White Light

Ultraviolet
Upgradeable

Three-channel Visible Fluorescence Detection

The Azure 400 offers 3 channel fluorescent detection for Westerns, chemiluminescent detection with the speed and sensitivity of film, and imaging for standard EtBr and protein gels. In addition to multiplex Westerns, the Azure 400 is a high performance chemiluminescent imager and gel documentation workstation. This fluorescent imager provides accurate and fast chemiluminescent detection, as well as the sensitivity, dynamic range, and linearity needed for quantitative blot analysis.

Get the best picture every time, and stop wasting money on film! UV, color imaging, blue-excited DNA dyes, Coomassie gel imaging, and more. The Azure 400 is a multichannel, multimodal imager with visible fluorescence, visible light, and UV excitation channels.

The Azure 400 is capable of three-channel visible fluorescence detection, which enables sensitive multiplex detection of Wester blots, fluorescent biomolecules and Cy2/Cy3/Cy5 or similar fluorochromes. This fluorescent imager allows users to simultaneously image and quantify up to three different targets.

 

Fluorescent protein in native gel.
Fluorescent protein in native gel imaged with an Azure imager
GFP- and mCherry-expressing E. coli.
GFP- and mCherry-expressing E. coli. imaged with an Azure Imager
GFP-expressing marijuana
GFP-expressing Marijuana imaged with an Azure imager

Sensitivity Signal Surpasses Film

Chemiluminescence detection that surpasses X-ray film detection in sensitivity and dynamic range

Quantitative chemiluminescent imaging

Outperforms film performance while generates a quantitative, wide dynamic range image. Indicates eventual signal saturation for accurate quantitation.

The imaging chamber replaces the darkroom and film developers to generate a digital image including true color molecular weight marker. Obtain your images immediately and be ready to quantify bands in the analysis software.

Figure 1. Saturation detection prevents errors in quantitation. The same Western Blot was imaged on both x-ray film and the Azure Imaging System. The Azure system detects when CCD saturation occurs and calculates an auto-exposure time to avoid saturated bands.
Intuitive capture software controlled by a wide touchscreen

The Azure 400 is a fluorescent imager that provides high-resolution imaging is ideal for publication purposes, as well as higher pixel well cap for higher dynamic range.

Use the preset protocols of the comprehensive list of typical detections, or generate your own custom protocols combining any excitation source, emission filter, image overlay, lens aperture, exposure time, focus, distance to the camera, and resolution.

Compact Design

At 42 x 56 x 33 cm, the small size translates into more room on your bench

Wide Dynamic Range

Capture images of visible fluorescence Western blot in multiplex, ECL Western blot, biomolecules tagged with fluorescent labels in gels, fluorescent and colorimetric stains in gels, Petri dishes, TLC, etc. True Color Imaging of translucent and opaque samples.

Artistic overlay of a scientist using an Azure 600 Western blot imaging system

Modular fluorescent imager to support your research

Upgradeable to detect NIR700/NIR800

The Azure 400 can be field upgraded to the Azure 600 for applications detecting NIR700/NIR800 or similar fluorochromes.

Connectivity through WI-FI, Ethernet, and USB with Microsoft Windows10 OS
Azure 400 Visible fluorescent imager
The Azure 400 offers:


Flexibility—Sensitivity and performance for fluorescence, chemiluminescence, trans-UV & visible, and true-color imaging with 4.8 OD dynamic range. High resolution CCD cameras with fast lens options, adjustable lens and illumination settings, trays with adjustable distance to the camera, and auto-detection and pre-calibrated focus.

Quantitative accuracy—Designed for generating publication quality images and easy quantitation. Our reagents, imaging system, and software work seamlessly together to help you follow best practices for Western Blot publication.

Intelligent workflow—Our user interface allows total customization over imaging protocols, while ensuring repeatability from sample to sample. Our systems feature Auto-Focus, Auto-Illumination and Filter Control, and Auto-Image Capture. An integrated touchscreen allows ease of use and it can easily be controlled by an external PC, if required.

Data integrity—Azure Biosystems meets the standards for publication in all major journals, and additionally offers software to enable 21 CFR Part 11 Compliance.

Literature

Document TypeDescription
BrochureAzure Imager BrochureDOWNLOAD
BrochureAzure Imaging Systems Model ConfigurationsDOWNLOAD
User ManualAzure Imaging Systems User ManualDOWNLOAD

Compare Western Blot Imaging Systems

Choose the configuration that supports today’s studies, while maintaining the option to upgrade later on

Upgrades available for chemiluminescence, NIR fluorescence and RGB fluorescence.

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