Visible Fluorescence

Azure 400 is the most flexible solution to detect three fluorescent targets in the visible range in multiplex, ECL WBs, and do all your gel documentation.

Three-channel Visible Fluorescence Detection

The three-channel Visible Fluorescence detection enables sensitive multiplex detection of WBs, fluorescent biomolecules and Cy2/Cy3/Cy5 or similar fluorochromes. Image and quantify up to three different targets simultaneously.

 

Fluorescent Western Blot of STAT1 and phospho-STAT1
Fluorescent Western Blot of STAT1 and phospho-STAT1. The blot was probed with anti-phospho-STAT1 and anti-STAT1 followed by fluorescent secondary antibodies, and then imaged on Azure Imaging system. Top right is the green channel, using IR-800; top left is the image of the red channel, using IR-700. Bottom image is both channels merged.
Two-fold Serial Dilution
Two-fold serial dilutions of AzureSpectra-800 labeled antibody were spotted on a nitrocellulose dot-blot, and imaged in the 800nm channel for 20 seconds
Fluorescent protein in native gel.
Fluorescent protein in native gel imaged with an Azure imager
GFP- and mCherry-expressing E. coli.
GFP- and mCherry-expressing E. coli. imaged with an Azure Imager

Sensitivity Signal Surpasses Film

Chemiluminescence detection that surpasses X-ray film detection in sensitivity and dynamic range

Quantitative chemiluminescent imaging

Outperforms film performance while generates a quantitative, wide dynamic range image. Indicates eventual signal saturation for accurate quantitation.

The imaging chamber replaces the darkroom and film developers to generate a digital image including true color molecular weight marker. Obtain your images immediately and be ready to quantify bands in the analysis software.

Figure 1. Saturation detection prevents errors in quantitation. The same Western Blot was imaged on both x-ray film and the Azure Imaging System. The Azure system detects when CCD saturation occurs and calculates an auto-exposure time to avoid saturated bands.
Intuitive capture software controlled by a wide touchscreen

Every Azure Imaging System provides high-resolution imaging that is ideal for publication purposes, as well as higher pixel well cap for higher dynamic range.

Use the preset protocols of the comprehensive list of typical detections, or generate your own custom protocols combining any excitation source, emission filter, image overlay, lens aperture, exposure time, focus, distance to the camera, and resolution.

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Upgradeable to Best Cater to Your Studies

Upgradeable to include three RGB fluorescence channels

Upgradeable to include two Near-Infrared fluorescence channels – The Azure 400 can be field upgraded to the Azure 600 for applications detecting NIR700/NIR800 or similar fluorochromes.

Dynamic Range of 4.82 orders of magnitude

Capture images of visible fluorescence WB in multiplex, ECL WB, biomolecules tagged with fluorescent labels in gels, fluorescent and colorimetric stains in gels, Petri dishes, TLC, etc. True Color Imaging of translucent and opaque samples.

400® | The Visible Fluorescence Virtuoso
RGB
Chemi
Blue Light
white light
Ultraviolet
Upgradeable
Connectivity through WI-FI, Ethernet, and USB in a Microsoft Windows10 OS
Azure 500
Each Azure Imaging System offers:


Flexibility—Sensitivity and performance for fluorescence, chemiluminescence, trans-UV & visible, and true-color imaging with 4.8 OD dynamic range. High resolution CCD cameras with fast lens options, adjustable lens and illumination settings, trays with adjustable distance to the camera, and auto-detection and pre-calibrated focus.

Quantitative accuracy—Designed for generating publication quality images and easy quantitation. Our reagents, imaging system, and software work seamlessly together to help you follow best practices for Western Blot publication.

Intelligent workflow—Our user interface allows total customization over imaging protocols, while ensuring repeatability from sample to sample. Our systems feature Auto-Focus, Auto-Illumination and Filter Control, and Auto-Image Capture. An integrated touchscreen allows ease of use and it can easily be controlled by an external PC, if required.

Data integrity—Azure Biosystems meets the standards for publication in all major journals, and additionally offers software to enable 21 CFR Part 11 Compliance.

Literature

Document TypeDescription
BrochureAzure Imager BrochureDOWNLOAD
BrochureAzure Imaging Systems Model ConfigurationsDOWNLOAD
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