Chemiluminescent Western Blot Imaging System

Say goodbye to the darkroom with the Azure 300.

Chemiluminescence for the Best Western Blots

Figure 1.4. Chemiluminescent Western blotting-  one signal, one protein. In chemiluminescent detection, the antigen-primary antibody complex is bound by a secondary antibody conjugated to an enzyme, such as horseradish peroxidase (HRP). The enzyme catalyzes a reaction that generates light in the presence of a luminescent substrate, and the light can be detected either by exposure to x-ray film or by a CCD-based imaging system.

The Azure 300 offers digital chemiluminescent detection at a sensitivity equal to film,  without all the hassles. Simply place your Western blot into the system and acquire the image with the click of a button.

 

Quantitative Chemiluminescent Imaging

Outperforms film performance while generates a quantitative, wide dynamic range image. Indicates eventual signal saturation for accurate quantitation.

The imaging chamber replaces the darkroom and film developers to generate a digital image including true color molecular weight marker. Obtain your images immediately and be ready to quantify bands in the analysis software.

Figure 1. Saturation detection prevents errors in quantitation. The same Western Blot was imaged on both x-ray film and the Azure Imaging System. The Azure system detects when CCD saturation occurs and calculates an auto-exposure time to avoid saturated bands.
Compact Design

At 42 x 56 x 33 cm, the small size translates into more room on your bench

Intuitive capture software controlled by a wide touchscreen

Every Azure Imaging System provides high-resolution imaging that is ideal for publication purposes, as well as higher pixel well cap for higher dynamic range.

Use the preset protocols of the comprehensive list of typical detections, or generate your own custom protocols combining any excitation source, emission filter, image overlay, lens aperture, exposure time, focus, distance to the camera, and resolution.

Footprint of the Azure 600® Imager vs. similar system (dotted line)
Upgrade to Add a Q Module

Add visible and/or NIR fluorescent detection capabilities as your needs evolve

Dynamic Range of 4.82 orders of magnitude

4.8 OD Dynamic Range

300® | The Darkroom Eliminator
Chemi
Blue Light
white light
Ultraviolet
Upgradeable
Connectivity through WI-FI, Ethernet, and USB in a Microsoft Windows10 OS
Azure 300
Each Azure Imaging System offers:


Flexibility—Sensitivity and performance for fluorescence, chemiluminescence, trans-UV & visible, and true-color imaging with 4.8 OD dynamic range. High resolution CCD cameras with fast lens options, adjustable lens and illumination settings, trays with adjustable distance to the camera, and auto-detection and pre-calibrated focus.

Quantitative accuracy—Designed for generating publication quality images and easy quantitation. Our reagents, imaging system, and software work seamlessly together to help you follow best practices for Western Blot publication.

Intelligent workflow—Our user interface allows total customization over imaging protocols, while ensuring repeatability from sample to sample. Our systems feature Auto-Focus, Auto-Illumination and Filter Control, and Auto-Image Capture. An integrated touchscreen allows ease of use and it can easily be controlled by an external PC, if required.

Data integrity—Azure Biosystems meets the standards for publication in all major journals, and additionally offers software to enable 21 CFR Part 11 Compliance.

Literature

Document TypeDescription
BrochureAzure Imager BrochureDOWNLOAD
BrochureAzure Imaging Systems Model ConfigurationsDOWNLOAD
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