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TotalStain Q Sample

Western Blotting Reagent Samples Looking for the right Western blotting reagents? You’ve come to the right spot! Azure Biosystems has a full range of transfer buffers, substrates, and blocking buffers

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TotalStain Q (NC)

Fluorescent Total Protein Stain for Western blots on nitrocellulose membranes

Accurate and quantitative fluorescent total protein stain to perform normalization of Western blots. Blots can be imaged on both camera-based and scanner instruments with green channel or Safe-Dyes channel.

TotalStain Q only adds a short 24-minute step to your Western blot protocol, between protein transfer and blocking buffer incubation. It is compatible with subsequent Western blot detection using chemiluminescence or Near Infrared (NIR) fluorescence.

  • Kit contains enough material for 15 membranes

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TotalStain Q (PVDF)

Accurate and quantitative fluorescent total protein stain to perform normalization of Western blots imaged on both camera-based and laser-based instruments with green channel or Safe-Dyes channel. Compatible with subsequent Western Blot detection using chemiluminescence or Near Infrared (NIR) fluorescence. Kit is good for 15 membranes.

The linear range between 1-50 μg of lysate makes it ideal for normalization of low-expressed proteins that require high loads of protein to reach the sensitivity for the detection of the target. TotalStain Q solves the issue of housekeeping proteins that lack linearity and normalization accuracy at high protein loads.

  • Simple 20-minute dye-and-wash protocol
  • Wide linear dynamic range across lysates of various tissue types
  • Compatible with both chemiluminescent and NIR fluorescence

View associated documents >

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Gel Electrophoresis Steps

While the specific steps of gel electrophoresis may differ somewhat between running DNA/RNA gels and protein gels, overall, the steps are the same. In this post, we’ll go over the five steps

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Western Blotting Reagents Roundup – November 2022

The Reagent Roundup is made of brief summaries of publications in which researchers used Azure Biosystems reagents for Western blotting and Western blot quantitation in their studies. It is published every quarter. This quarter’s Reagent

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Western Blot Imagers

Azure Imaging Systems What Can You See with Our Imagers? Western Blots, Gels, Petri dishes, Tissue sections, Small animal models, Plants, and MORE! In-gel Fluorescence with GFP and TAMRA In-gel

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Azure 500

Ultimate Near-infrared Fluorescent Imager Azure 500 AZI500-01 The Azure 500 is a NIR fluorescent imager that’s the best solution for sensitive near-infrared and ECL Western blots, and gel documentation. Multichannel,

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Western Blotting Reagents Roundup – July 2022

The Reagent Roundup is made of brief summaries of publications in which researchers used Azure Biosystems reagents for Western blotting and Western blot quantitation in their studies. It is published every quarter. This quarter’s Reagent

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