Accurate and quantitative fluorescent total protein stain to perform normalization of Western blots. Blots can be imaged on both camera-based and scanner instruments with green channel or Safe-Dyes channel.
TotalStain Q only adds a short 24-minute step to your Western blot protocol, between protein transfer and blocking buffer incubation. It is compatible with subsequent Western blot detection using chemiluminescence or Near Infrared (NIR) fluorescence.
Accurate and quantitative fluorescent total protein stain to perform normalization of Western blots imaged on both camera-based and laser-based instruments with green channel or Safe-Dyes channel. Compatible with subsequent Western Blot detection using chemiluminescence or Near Infrared (NIR) fluorescence. Kit is good for 15 membranes.
The linear range between 1-50 μg of lysate makes it ideal for normalization of low-expressed proteins that require high loads of protein to reach the sensitivity for the detection of the target. TotalStain Q solves the issue of housekeeping proteins that lack linearity and normalization accuracy at high protein loads.
Total protein normalization (TPN) is used to quantify the abundance of the protein of interest, without having to rely on housekeeping genes. It is usually done by incubating the membrane
Far from being an “is-it-there-or-not” technique, modern digital detection instruments can make Western blotting reproducible and quantitative. By working within the linear dynamic range of your detection method and normalizing